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Preparation and surface characterization of plasma-treated and biomolecular-micropatterened polymer substrates
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Text
TitleInfo
Title
Preparation and surface characterization of plasma-treated and biomolecular-micropatterened polymer substrates
Identifier
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http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.13476
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ETD_173
Identifier
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doi:10.7282/T3280834
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(authority = ISO 639-3:2007);
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English
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theses
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Topic
Chemistry and Chemical Biology
Subject
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Topic
Tissue engineering
Subject
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Topic
Biomedical engineering
Abstract
(type = abstract)
A micropatterning process creates distinct microscale domains on substrate surfaces that differ from the surfaces' original chemical/physical properties. Numerous micropatterning methods exist, each having relative advantages and disadvantages in terms of cost, ease, reproducibility, and versatility. Polymeric surfaces micropatterned with biomolecules have many applications, but are specifically utilized in tissue engineering as cell scaffolds that attempt to controlled tissue generation in vivo and ex vivo. As the physical and chemical cues presented by micropatterned substrates control resulting cellular behavior, characterization of these cues via surface-sensitive analytical techniques is essential in developing cell scaffolds that mimic complex in vivo physicochemical environments.
The initial focus of this thesis is the chemical and physical characterization of plasma-treated, microcontact-printed (µCP) polymeric substrates used to direct nerve cell behavior. Unmodified and oxygen plasma-treated poly(methyl methacrylate) (PMMA) substrates were analyzed by surface sensitive techniques to monitor plasma-induced chemical and physical modifications. Additionally, protein-micropattern homogeneity and size were microscopically evaluated. Lastly, poly(dimethylsiloxane) (PDMS) stamps and contaminated PMMA substrates were characterized by spectroscopic and microscopic methods to identify a contamination source during microcontact printing.
The final focus of this thesis is the development of microscale plasma-initiated patterning (µPIP) as a versatile, reproducible micropatterning method. Using µPIP, polymeric substrates were micropatterned with several biologically relevant inks. Polymeric substrates were characterized following µPIP by surface-sensitive techniques to identify the technique's underlying physical and chemical bases. In addition, neural stem cell response to µPIP-generated laminin micropatterns was microscopically and biologically evaluated. Finally, enhanced versatility of µPIP in generating microscale poly-L-lysine gradients was demonstrated.
The initial focus of this thesis is the chemical and physical characterization of plasma-treated, microcontact-printed (µCP) polymeric substrates used to direct nerve cell behavior. Unmodified and oxygen plasma-treated poly(methyl methacrylate) (PMMA) substrates were analyzed by surface sensitive techniques to monitor plasma-induced chemical and physical modifications. Additionally, protein-micropattern homogeneity and size were microscopically evaluated. Lastly, poly(dimethylsiloxane) (PDMS) stamps and contaminated PMMA substrates were characterized by spectroscopic and microscopic methods to identify a contamination source during microcontact printing.
The final focus of this thesis is the development of microscale plasma-initiated patterning (µPIP) as a versatile, reproducible micropatterning method. Using µPIP, polymeric substrates were micropatterned with several biologically relevant inks. Polymeric substrates were characterized following µPIP by surface-sensitive techniques to identify the technique's underlying physical and chemical bases. In addition, neural stem cell response to µPIP-generated laminin micropatterns was microscopically and biologically evaluated. Finally, enhanced versatility of µPIP in generating microscale poly-L-lysine gradients was demonstrated.
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electronic resource
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xvi, 194 p. : ill.
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Ph.D.
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Includes bibliographical references.
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by Bryan Alfred Langowski
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Langowski
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Bryan Alfred
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Bryan Alfred Langowski
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Uhrich
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Kathryn
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Kathryn E. Uhrich
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Madey
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Theodore
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Theodore E. Madey
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Lee
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Jeehiun
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Jeehiun Lee
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Moghe
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Prabhas
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Prabhas Moghe
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Rutgers University
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Graduate School-New Brunswick
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2007
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2007
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xx
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Graduate School - New Brunswick Electronic Theses and Dissertations
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rucore19991600001
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ETD doctoral
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I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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