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Biomaterials and microfabrication techniques for improved peripheral nerve regeneration
Descriptive
TitleInfo
Title
Biomaterials and microfabrication techniques for improved peripheral nerve regeneration
Name
(type = personal)
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Song
NamePart
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Minjung
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Minjung Song
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(authority = RULIB)
author
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Uhrich
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Kathryn
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Advisory Committee
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Kathryn E Uhrich
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chair
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Kim
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Sobin
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Advisory Committee
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Sobin Kim
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internal member
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Shreiber
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David
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Advisory Committee
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David I Shreiber
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internal member
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Buettner
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Helen
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Advisory Committee
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Helen M Buettner
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internal member
Name
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(type = family)
Firestein
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Bonnie
Affiliation
Advisory Committee
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Bonnie L Firestein
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outside member
Name
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NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
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NamePart
Graduate School-New Brunswick
Role
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school
TypeOfResource
Text
Genre
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theses
OriginInfo
DateCreated
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2007
DateOther
(qualifier = exact);
(type = degree)
2007
Language
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(authority = ISO 639-3:2007);
(type = text)
English
PhysicalDescription
Form
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electronic
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application/pdf
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text/xml
Extent
xxi, 170 pages
Abstract
(type = abstract)
Following severe nerve injuries, surgery is required to physically reconnect the injured nerve for regeneration. Auto-grafting is typically considered to be the most effective method, but is problematic in terms of the limited supply of suitable nerves. Various artificial nerve materials are under investigation, but the lack of directional cues, biomolecular support, and the effects of inflammation at injury site limit the complete regeneration.
Protein micropatterned surfaces generated by microcontact printing and micron scale plasma-initiated patterning allow the cellular environment to be manipulated by providing chemical and physical cues to neural cells. The first objective of this study is to modify these cues to control neural cell growth. Laminin, a common chemical cue, was modified by RGD conjugation to improve Schwann cell adhesion. This chemical cue modification resulted in improved Schwann cell adhesion and guidance. To change the physical cues, various micropattern dimensions ranging from 10 to 50 µm stripes with a consistent 40 µm space were prepared; neuron growth and guidance was optimal on the 40 µm striped pattern.
The second objective was to generate a novel biomolecular gradient system using microfluidic techniques. Controlling the microfluidic channel dimensions, flow rate, and collagen gel properties created a three-dimensional, adhesive gradient within a collagen gel. The specific impact of the biomolecular gradient on neuron growth can be evaluated.
The final objective was to utilize non-steroidal anti-inflammatory drug (NSAID) based - poly(anhydride-esters) (PAE) for nerve regeneration. These polymers are biodegradable and release NSAIDs upon hydrolytic degradation. The compatibility of four polymers with neurons and Schwann cells was evaluated; the salicylic acid-based PAE (SAA) proved the most biocompatible. The SAA nerve guidance conduit was fabricated and conduit properties characterized. Sufficient mechanical strength and biocompatibility of the conduit was demonstrated.
This work demonstrates that nerve regeneration can be enhanced and improved by controlling neural cell guidance using micropatterned surfaces, fabricating a biomolecular gradient system using a microfluidic technique to investigate its impact on neuron growth, and applying drug-containing polymers as a nerve guidance conduit.
Protein micropatterned surfaces generated by microcontact printing and micron scale plasma-initiated patterning allow the cellular environment to be manipulated by providing chemical and physical cues to neural cells. The first objective of this study is to modify these cues to control neural cell growth. Laminin, a common chemical cue, was modified by RGD conjugation to improve Schwann cell adhesion. This chemical cue modification resulted in improved Schwann cell adhesion and guidance. To change the physical cues, various micropattern dimensions ranging from 10 to 50 µm stripes with a consistent 40 µm space were prepared; neuron growth and guidance was optimal on the 40 µm striped pattern.
The second objective was to generate a novel biomolecular gradient system using microfluidic techniques. Controlling the microfluidic channel dimensions, flow rate, and collagen gel properties created a three-dimensional, adhesive gradient within a collagen gel. The specific impact of the biomolecular gradient on neuron growth can be evaluated.
The final objective was to utilize non-steroidal anti-inflammatory drug (NSAID) based - poly(anhydride-esters) (PAE) for nerve regeneration. These polymers are biodegradable and release NSAIDs upon hydrolytic degradation. The compatibility of four polymers with neurons and Schwann cells was evaluated; the salicylic acid-based PAE (SAA) proved the most biocompatible. The SAA nerve guidance conduit was fabricated and conduit properties characterized. Sufficient mechanical strength and biocompatibility of the conduit was demonstrated.
This work demonstrates that nerve regeneration can be enhanced and improved by controlling neural cell guidance using micropatterned surfaces, fabricating a biomolecular gradient system using a microfluidic technique to investigate its impact on neuron growth, and applying drug-containing polymers as a nerve guidance conduit.
Note
(type = degree)
Ph.D.
Note
(type = bibliography)
Includes bibliographical references.
Subject
(authority = RUETD)
Topic
Biomedical Engineering
Subject
(authority = ETD-LCSH)
Topic
Nervous system
Subject
(authority = ETD-LCSH)
Topic
Regeneration (Biology)
Subject
(authority = ETD-LCSH)
Topic
Nerve grafting
RelatedItem
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TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier
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rucore19991600001
Identifier
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http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.13833
Identifier
ETD_229
Identifier
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doi:10.7282/T38W3DRH
Location
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(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Genre
(authority = ExL-Esploro)
ETD doctoral
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The author owns the copyright to this work.
Copyright
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Copyright protected
Availability
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Open
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Name
Minjung Song
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Affiliation
Rutgers University. Graduate School-New Brunswick
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Non-exclusive ETD license
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Author Agreement License
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I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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