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Influence of environmental factors and the host's susceptibility on the development of listeria monocytogenes infection in the guinea pig model
Descriptive
TitleInfo
Title
Influence of environmental factors and the host's susceptibility on the development of listeria monocytogenes infection in the guinea pig model
Name
(type = personal)
NamePart
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Pang
NamePart
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Hoan-Jen Eunice
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Hoan-Jen Pang
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author
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(type = family)
Matthews
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Karl
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Advisory Committee
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Karl R Matthews
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chair
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Montville
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Thomas
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Advisory Committee
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Thomas Montville
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internal member
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(type = family)
Tchikindas
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Mikhail
Affiliation
Advisory Committee
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Mikhail Tchikindas
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internal member
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(type = family)
Fratamico
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(type = given)
Pina
Affiliation
Advisory Committee
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Pina Fratamico
Role
RoleTerm
(authority = RULIB)
outside member
Name
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NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
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NamePart
Graduate School-New Brunswick
Role
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(authority = RULIB)
school
TypeOfResource
Text
Genre
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theses
OriginInfo
DateCreated
(qualifier = exact)
2007
DateOther
(qualifier = exact);
(type = degree)
2007
Language
LanguageTerm
(authority = ISO 639-3:2007);
(type = text)
English
PhysicalDescription
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electronic
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application/pdf
InternetMediaType
text/xml
Extent
viii, 97 pages
Abstract
(type = abstract)
Listeria monocytogenes is an intracellular pathogen commonly associated with ready-to-eat foods. Using the guinea pig model, the impact of environmental factors on the infectivity of L. monocytogenes, as well as the impact of a host's immunity, on the development of listerial infection was investigated. Experiments were conducted to determine whether exposure to food environment and improper handling conditions impact the infectivity of L. monocytogenes. Results indicated that the virulence of L. monocytogenes was not influenced by exposure of the pathogen to food or improper handling conditions. In young adult guinea pigs orally challenged with 10² L. monocytogenes, a 27% infection rate occurred.
Further studies were conducted to determine the impact of immunomodulation of a host's immunity on the development of listerial infection using aged guinea pigs. Flow cytometry analysis showed that daily supplementation with vitamin E significantly increased the level of CD8+ T cells, while cyclosporine A (CsA) treatment resulted in a 25% decrease of CD8+ T cells. HPLC analysis showed that short-term supplementation with vitamin E resulted in an overall 1-fold increase in the plasma alpha-tocopherol concentration. In low-dose (10² CFU) challenged animals, 50% of the control-group animals became infected. Only 22% of animals receiving the orthomolecular dose of vitamin E became infected, whereas animals that were immunosuppressed had an infection rate of 89%. In the immunosuppressed group three animals (16%) developed listerial infection with a quantifiable bacterial level of 0.3-3 log CFU g(-1) of organ in the spleen and liver. In the high-dose study, the population of L. monocytogenes was consistently 1 log CFU lower in the spleen and liver of animals in the vitamin E group as compared to the control and CsA-treated groups. The level of L. monocytogenes was not significantly different between the control and CsA-treated groups. The results suggest that immunomodulation of the host can influence listerial infection within an aged population of guinea pigs. From a public health perspective, the research indicates that a
low oral dose of L. monocytogenes may pose a human health risk.
Further studies were conducted to determine the impact of immunomodulation of a host's immunity on the development of listerial infection using aged guinea pigs. Flow cytometry analysis showed that daily supplementation with vitamin E significantly increased the level of CD8+ T cells, while cyclosporine A (CsA) treatment resulted in a 25% decrease of CD8+ T cells. HPLC analysis showed that short-term supplementation with vitamin E resulted in an overall 1-fold increase in the plasma alpha-tocopherol concentration. In low-dose (10² CFU) challenged animals, 50% of the control-group animals became infected. Only 22% of animals receiving the orthomolecular dose of vitamin E became infected, whereas animals that were immunosuppressed had an infection rate of 89%. In the immunosuppressed group three animals (16%) developed listerial infection with a quantifiable bacterial level of 0.3-3 log CFU g(-1) of organ in the spleen and liver. In the high-dose study, the population of L. monocytogenes was consistently 1 log CFU lower in the spleen and liver of animals in the vitamin E group as compared to the control and CsA-treated groups. The level of L. monocytogenes was not significantly different between the control and CsA-treated groups. The results suggest that immunomodulation of the host can influence listerial infection within an aged population of guinea pigs. From a public health perspective, the research indicates that a
low oral dose of L. monocytogenes may pose a human health risk.
Note
(type = degree)
Ph.D.
Note
(type = bibliography)
Includes bibliographical references (p. 89-91).
Subject
(authority = RUETD)
Topic
Food Science
Subject
(authority = ETD-LCSH)
Topic
Listeria monocytogenes
Subject
(authority = ETD-LCSH)
Topic
Biological response modifiers
RelatedItem
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TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier
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rucore19991600001
Identifier
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http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.13843
Identifier
ETD_176
Identifier
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doi:10.7282/T3XK8G05
Location
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(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
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ETD doctoral
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Rights
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The author owns the copyright to this work.
Copyright
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Copyright protected
Availability
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Open
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Name
Hoan-Jen Pang
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Copyright holder
Affiliation
Rutgers University. Graduate School-New Brunswick
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Permission or license
Detail
Non-exclusive ETD license
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License
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Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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