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Functional analysis of RAB-10 and its interacting partner EHBP-1 during endocytosis in the Caenorhabditis elegans intestine

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TitleInfo (displayLabel = Citation Title); (type = uniform)
Title
Functional analysis of RAB-10 and its interacting partner EHBP-1 during endocytosis in the Caenorhabditis elegans intestine
Name (ID = NAME001); (type = personal)
NamePart (type = family)
Chen
NamePart (type = given)
Chih-Hsiung
NamePart (type = date)
1967-
DisplayForm
Chih-Hsiung Chen
Role
RoleTerm (authority = RUETD)
author
Name (ID = NAME002); (type = personal)
NamePart (type = family)
Driscoll
NamePart (type = given)
Monica
Affiliation
Advisory Committee
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Monica Driscoll
Role
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chair
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NamePart (type = family)
Grant
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Barth
Affiliation
Advisory Committee
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Barth D. Grant
Role
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internal member
Name (ID = NAME004); (type = personal)
NamePart (type = family)
Rongo
NamePart (type = given)
Christopher
Affiliation
Advisory Committee
DisplayForm
Christopher Rongo
Role
RoleTerm (authority = RULIB)
internal member
Name (ID = NAME005); (type = personal)
NamePart (type = family)
Kramer
NamePart (type = given)
Sunita
Affiliation
Advisory Committee
DisplayForm
Sunita Kramer
Role
RoleTerm (authority = RULIB)
outside member
Name (ID = NAME006); (type = personal)
NamePart (type = date)
1967-
Role
Name (ID = NAME007); (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (ID = NAME008); (type = corporate)
NamePart
Graduate School - New Brunswick
Role
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school
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Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (qualifier = exact)
2007
DateOther (qualifier = exact); (type = degree)
2007
Language
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English
PhysicalDescription
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electronic
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application/pdf
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text/xml
Extent
xxii, 288 pages
Abstract
The endocytic pathway of eukaryotes is essential for the internalization and trafficking of macromolecules, fluid, membranes, and membrane proteins. One of the most enigmatic aspects of this process is endocytic recycling, the return of macromolecules (often receptors) and fluid from endosomes to the plasma membrane. It has been previously shown that the EH-domain protein RME-1 is a critical regulator of endocytic recycling in worms and mammals. In my studies, the RAB-10 protein was identified as a key regulator of endocytic recycling upstream of RME-1 in polarized epithelial cells of the Caenorhabditis elegans intestine. rab-10 null mutant intestinal cells accumulate abnormally abundant RAB-5-positive early endosomes, some of which are enlarged by more than 10-fold. Conversely most RME-1-positive recycling endosomes are lost in rab-10 mutants. The abnormal early endosomes in rab-10 mutants accumulate basolaterally recycling transmembrane cargo molecules and basolaterally recycling fluid, consistent with a block in basolateral transport. These results indicate a role for RAB-10 in basolateral recycling upstream of RME-1.
In a yeast two-hybrid screen for RAB-10-interacting proteins, eight candidates with RAB-10-interacting ability were identified. They are EHBP-1, HUM-2, GCK-2, CNT-1, F52E1.13, Y82E9BR.21, ZK1248.10, and F20D1.2. In addition, 5/8 candidates identified interacted with RAB-8 and RAB-10 but not with RAB-5, -7, -11, or -35 in the two-hybrid assay. One of the RAB-10-interacting candidates, EHBP-1, an actin-cyskeleton bundling protein, was revealed to function together with RAB-10 in vivo. Knockdown of EHBP-1 function by RNAi produced a rab-10-like phenotype in the intestine. In addition, EHBP-1 was found to be required for endosomal membrane association of RAB-10 and actin association with the enlarged endosomes of rab-10 mutants. I conclude that RAB-10 and EHBP-1 likely function together in basolateral endocytic recycling pathway of the C. elegans intestine.
Note (type = degree)
Ph.D.
Note (type = bibliography)
Includes bibliographical references (p. 272-286).
Subject (ID = SUBJ1); (authority = RUETD)
Topic
Cell and Developmental Biology
Subject (ID = SUBJ2); (authority = ETD-LCSH)
Topic
Caenorhabditis elegans
Subject (ID = SUBJ3); (authority = ETD-LCSH)
Topic
Endocytosis
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Identifier (type = hdl)
http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.15796
Identifier
ETD_328
Location
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NjNbRU
Identifier (type = doi)
doi:10.7282/T3V40VN3
Genre (authority = ExL-Esploro)
ETD doctoral
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The author owns the copyright to this work.
Copyright
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Availability
Status
Open
AssociatedEntity (AUTHORITY = rulib); (ID = 1)
Name
CHIH-HSIUNG CHEN
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
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Type
Permission or license
Detail
Non-exclusive ETD license
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Author Agreement License
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I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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