RUcore: Rutgers University Community Repository
Adaptation of the Drosophila circadian clock to seasonal changes in temperature and photoperiod
Descriptive
TitleInfo
(displayLabel = Citation Title);
(type = uniform)
Title
Adaptation of the Drosophila circadian clock to seasonal changes in temperature and photoperiod
Name
(ID = NAME001);
(type = personal)
NamePart
(type = family)
Chen
NamePart
(type = given)
Wen-Feng
DisplayForm
Wen-Feng Chen
Role
RoleTerm
(authority = RULIB)
author
Name
(ID = NAME002);
(type = personal)
NamePart
(type = family)
GUNDERSON
NamePart
(type = given)
SAMUEL
Affiliation
Advisory Committee
DisplayForm
SAMUEL I GUNDERSON
Role
RoleTerm
(authority = RULIB)
chair
Name
(ID = NAME003);
(type = personal)
NamePart
(type = family)
Williams
NamePart
(type = given)
Julie
Affiliation
Advisory Committee
DisplayForm
Julie A Williams
Role
RoleTerm
(authority = RULIB)
internal member
Name
(ID = NAME004);
(type = personal)
NamePart
(type = family)
KILEDJIAN
NamePart
(type = given)
MEGERDIT
Affiliation
Advisory Committee
DisplayForm
MEGERDIT KILEDJIAN
Role
RoleTerm
(authority = RULIB)
outside member
Name
(ID = NAME005);
(type = corporate)
NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
(ID = NAME006);
(type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm
(authority = RULIB)
school
TypeOfResource
Text
Genre
(authority = marcgt)
theses
OriginInfo
DateCreated
(qualifier = exact)
2007
DateOther
(qualifier = exact);
(type = degree)
2007
Language
LanguageTerm
English
PhysicalDescription
Form
(authority = marcform)
electronic
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
xiii, 136 pages
Abstract
In Drosophila melanogaster, splicing of an intron in the 3' untranslated region of the period (per) mRNA (herein referred to as dmpi8) is enhanced at cold temperatures, leading to more rapid daily increases in per transcript levels and earlier "evening" activity. We show that shortening the photoperiod (day-length) enhances daily fluctuations in the splicing of dmpi8 and advances its cycle, whereas the amplitude of the clock-regulated daytime decline in splicing increases as temperatures rise. Our results identify a novel non-photic role for NORPA in the temperature regulated repression of dmpi8 splicing.
In related work I found that two haplotypes (termed VT1.1 and VT1.2) in the per 3' UTR with six natural polymorphisms showed differential splicing efficiency in inbred flies, transgenic flies, and S2 cells. The VT1.1 haplotype leads to better dmpi8 splicing and earlier evening activity. Splicing assays in S2 cells also suggest that efficient dmpi8 splicing promotes per mRNA export. Our results suggest that splicing of dmpi8 plays a major role for Drosophila in adapting to local climates.
This dissertation also shows that light acutely stimulates the expression of tim at cold but not warm temperatures and that this is regulated at the transcriptional level. Cold-specific stimulation of tim RNA abundance requires CLK or CYC but not PER, TIM, CRY, or the canonical phototransduction pathway. The photoinduction of tim is temporally restricted to the daily rising phase in tim mRNA levels, likely ensuring that sunrise does not prematurely stimulate tim expression during unseasonably cold long days.
My work suggests a model whereby temperature and day-length are integrated to set the phase of the clock in a seasonably appropriate manner. Namely, during cold short days, stimulation of per dmpi8 splicing and photoinduction of tim mRNA transcription ensures that both per and tim transcripts accumulate more rapidly, leading to Drosophila exhibiting preferential activity during the warmer daytime hours despite the earlier onset of dusk. Conversely, during warm long days, the inefficient splicing of dmpi8 and lack of light stimulated tim transcription delay evening activity such that flies avoid the deleterious effects of the hot midday hours.
In related work I found that two haplotypes (termed VT1.1 and VT1.2) in the per 3' UTR with six natural polymorphisms showed differential splicing efficiency in inbred flies, transgenic flies, and S2 cells. The VT1.1 haplotype leads to better dmpi8 splicing and earlier evening activity. Splicing assays in S2 cells also suggest that efficient dmpi8 splicing promotes per mRNA export. Our results suggest that splicing of dmpi8 plays a major role for Drosophila in adapting to local climates.
This dissertation also shows that light acutely stimulates the expression of tim at cold but not warm temperatures and that this is regulated at the transcriptional level. Cold-specific stimulation of tim RNA abundance requires CLK or CYC but not PER, TIM, CRY, or the canonical phototransduction pathway. The photoinduction of tim is temporally restricted to the daily rising phase in tim mRNA levels, likely ensuring that sunrise does not prematurely stimulate tim expression during unseasonably cold long days.
My work suggests a model whereby temperature and day-length are integrated to set the phase of the clock in a seasonably appropriate manner. Namely, during cold short days, stimulation of per dmpi8 splicing and photoinduction of tim mRNA transcription ensures that both per and tim transcripts accumulate more rapidly, leading to Drosophila exhibiting preferential activity during the warmer daytime hours despite the earlier onset of dusk. Conversely, during warm long days, the inefficient splicing of dmpi8 and lack of light stimulated tim transcription delay evening activity such that flies avoid the deleterious effects of the hot midday hours.
Note
(type = degree)
Ph.D.
Note
(type = bibliography)
Includes bibliographical references (p. 121-135).
Subject
(ID = SUBJ1);
(authority = RUETD)
Topic
Biochemistry
Subject
(ID = SUBJ2);
(authority = ETD-LCSH)
Topic
Circadian rhythms
Subject
(ID = SUBJ3);
(authority = ETD-LCSH)
Topic
Drosophila
RelatedItem
(type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier
(type = local)
rucore19991600001
Identifier
(type = hdl)
http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.15797
Identifier
ETD_295
Location
PhysicalLocation
(authority = marcorg);
(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T3ZW1MB2
Genre
(authority = ExL-Esploro)
ETD doctoral
Back to the top
Rights
RightsDeclaration
(AUTHORITY = GS);
(ID = rulibRdec0006)
The author owns the copyright to this work.
Copyright
Status
Copyright protected
Availability
Status
Open
AssociatedEntity
(AUTHORITY = rulib);
(ID = 1)
Name
Wen-Feng Chen
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
RightsEvent
(AUTHORITY = rulib);
(ID = 1)
Type
Permission or license
Detail
Non-exclusive ETD license
AssociatedObject
(AUTHORITY = rulib);
(ID = 1)
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Back to the top
Technical
Format
(TYPE = mime);
(VERSION = )
application/x-tar
FileSize
(UNIT = bytes)
3244544
Checksum
(METHOD = SHA1)
61598d118fe37c6d30d99d6ad4cb8d91c2149880
ContentModel
ETD
CompressionScheme
other
OperatingSystem
(VERSION = 5.1)
windows xp
Format
(TYPE = mime);
(VERSION = NULL)
application/x-tar
Back to the top
Statistical Profile