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Rare paternal plastid inheritance in arabidopsis

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TitleInfo (displayLabel = Citation Title); (type = uniform)
Title
Rare paternal plastid inheritance in arabidopsis
Name (ID = NAME001); (type = personal)
NamePart (type = family)
Azhagiri
NamePart (type = given)
Arun
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Arun Azhagiri
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author
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NamePart (type = family)
Maliga
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Pal
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Advisory Committee
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Pal Maliga
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chair
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Dooner
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Hugo
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Advisory Committee
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Hugo Dooner
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internal member
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Kerstetter
NamePart (type = given)
Randall
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Advisory Committee
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Randall G Kerstetter
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internal member
Name (ID = NAME005); (type = personal)
NamePart (type = family)
Messing
NamePart (type = given)
Joachim
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Advisory Committee
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Joachim Messing
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internal member
Name (ID = NAME006); (type = personal)
NamePart (type = family)
Padgett
NamePart (type = given)
Robert
Affiliation
Advisory Committee
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Robert W Padgett
Role
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outside member
Name (ID = NAME007); (type = corporate)
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Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (ID = NAME008); (type = corporate)
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Graduate School - New Brunswick
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school
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Text
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theses
OriginInfo
DateCreated (qualifier = exact)
2007
DateOther (qualifier = exact); (type = degree)
2007
Language
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English
PhysicalDescription
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electronic
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application/pdf
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text/xml
Extent
ix, 121 pages
Abstract
Plastids and mitochondria, in a standard genetic cross, are transmitted to the seed progeny by the maternal parent in Arabidopsis thaliana. My objective was to test, if exceptional pollen transmission of plastid occurs in Arabidopsis, a species in which no plastid DNA could be detected in pollen or sperm cells by cytological methods. The maternal parent was the nuclear male sterile (ms1-1/ms1-1), spectinomycin sensitive Ler ecotype and the pollen parent was the male fertile RLD-Spc1 plant carrying a plastid-encoded spectinomycin resistance mutation. I selected for exceptional pollen transmission in the progeny by spectinomycin resistance encoded in the paternal plastid DNA. I found that plastids, in general, are inherited maternally in Arabidopsis and rare events of paternal plastid transmission to the seed progeny occurs at a low (3.9 x 10-5) frequency. This observation extends previous reports in Nicotiana tabacum (family: Solanaceae) to a cruciferous species suggesting that low frequency paternal leakage of plastids via pollen may be universal in plants previously thought to exhibit strict maternal plastid inheritance.
Two components needed to accomplish this study were a plastid-encoded, spectinomycin resistant mutant as the pollen parent and plastid markers to identify the origin of the plastids in the hybrid seed progeny obtained from the crosses. To identify genetic markers in the Arabidopsis thaliana plastid genome (ptDNA) I amplified and sequenced the rpl2-psbA and rbcL-accD regions in 26 ecotypes. The two regions contained eight polymorphic sites including five insertions and/or deletions (indels) involving changes in the length of A or T mononucleotide repeats and three base substitutions. The 27 alleles provided a practical set of ptDNA markers for the commonly used RLD, Ler, Col and C24 ecotypes. I used a RLD-Spc1 line carrying a plastid-encoded spectinomycin resistant mutation as the pollen parent that was compatible with the Ler maternal parent.
Note (type = degree)
Ph.D.
Note (type = bibliography)
Includes bibliographical references.
Subject (ID = SUBJ1); (authority = RUETD)
Topic
Plant Biology
Subject (ID = SUBJ2); (authority = ETD-LCSH)
Topic
Plastids
Subject (ID = SUBJ3); (authority = ETD-LCSH)
Topic
Arabidopsis thaliana
Subject (ID = SUBJ4); (authority = ETD-LCSH)
Topic
Plant genetics
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Identifier (type = hdl)
http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.15779
Identifier
ETD_269
Location
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NjNbRU
Identifier (type = doi)
doi:10.7282/T3028S1S
Genre (authority = ExL-Esploro)
ETD doctoral
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The author owns the copyright to this work.
Copyright
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Copyright protected
Availability
Status
Open
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Name
Arun Azhagiri
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
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Non-exclusive ETD license
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Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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