RUcore: Rutgers University Community Repository
Dietary lycopene modulates prostate cancer biomarker genes in androgen-independent human prostate cancer (PC-3) cell line
Descriptive
TitleInfo
(displayLabel = Citation Title);
(type = uniform)
Title
Dietary lycopene modulates prostate cancer biomarker genes in androgen-independent human prostate cancer (PC-3) cell line
Name
(ID = NAME001);
(type = personal)
NamePart
(type = family)
Reyes
NamePart
(type = given)
Marynell D.
DisplayForm
Marynell D. Reyes
Role
RoleTerm
(authority = RUETD)
author
Name
(ID = NAME002);
(type = personal)
NamePart
(type = family)
Rafi
NamePart
(type = given)
Mohamed
Affiliation
Advisory Committee
DisplayForm
Mohamed M Rafi
Role
RoleTerm
(authority = RULIB)
chair
Name
(ID = NAME003);
(type = personal)
NamePart
(type = family)
Lachance
NamePart
(type = given)
Paul
Affiliation
Advisory Committee
DisplayForm
Paul A Lachance
Role
RoleTerm
(authority = RULIB)
internal member
Name
(ID = NAME004);
(type = personal)
NamePart
(type = family)
Daun
NamePart
(type = given)
Henryk
Affiliation
Advisory Committee
DisplayForm
Henryk Daun
Role
RoleTerm
(authority = RUETD)
dissertation committee member
Name
(ID = NAME005);
(type = corporate)
NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
(ID = NAME006);
(type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm
(authority = RULIB)
school
TypeOfResource
Text
Genre
(authority = marcgt)
theses
OriginInfo
DateCreated
(qualifier = exact)
2007
DateOther
(qualifier = exact);
(type = degree)
2007
Language
LanguageTerm
English
PhysicalDescription
Form
(authority = marcform)
electronic
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
x, 72 pages
Abstract
In the United States prostate cancer is the most commonly diagnosed cancer and is the second leading cause of death from malignancies in men. Dietary components have been recently targeted in the prevention and treatment of cancer, in combination with existing medical treatments for prostate cancer. Lycopene is a fat soluble red-orange carotenoid primarily consumed in foods containing tomatoes and tomato-derived products including tomato sauce, tomato paste and ketchup, with relatively smaller amounts in dried apricots, watermelon and pink grapefruit. Our objective is to determine whether lycopene treatment modulates prostate cancer biomarker genes in hormone-refractory human prostate cancer (PC-3) cell lines using Oligo GEArray® DNA Microarray which contains 263 genes involved in the prognosis and diagnosis of prostate cancer. Cell viability experiments determined that 25µM lycopene was the highest non-toxic treatment dose and therefore was selected for further experiments. Microarray image analysis demonstrated a decrease in the expression of transforming growth factor beta-2 (TGFβ-2), cyclin dependent kinase-9 (CDK-9), epidermal growth factor receptor (EGFR), B-cell lymphoma-2 (BCL-2), B-cell lymphoma-2 like 1 (BCL2L1), insulin-like growth factor 1 receptor (IGF1R), cyclin dependent kinase-7 (CDK-7), and breast cancer 1 (BRCA1) genes after the treatment of PC-3 cells with 25µM lycopene. Percent down-regulation calculated for TGFβ-2, CDK-9, EGFR, BCL-2, BCL2L1, IGF1R, CDK-7, and BRCA1 genes was 79%, 68%, 59%, 54%, 52%, 48%, 43%, and 38%, respectively, in lycopene-treated versus untreated samples. The modulated expressions of EGFR, IGF1R, BRCA1, CDK-9, TGFβ-2, CDK-7, and BCL-2 genes were validated using Real-Time Polymerase Chain Reaction (Real-Time PCR) and the results demonstarted a down-regulation of 72%, 61%, 56%, 56%, 44%, 34%, and 30%, respectively. Among all modulated prostate cancer biomarker genes, EGFR demonstrated the most consistent down-regulation in expression in our microarray and Real-Time PCR analyses. Protein expression analysis demonstrated that lycopene treatment also decreased EGFR protein expression in lycopene-treated PC-3 cells by 36%. These results indicate that the treatment of PC-3 cells with lycopene consistently modulates several prostate cancer biomarker genes. The present study clearly indicates that EGFR is down-regulated at the mRNA and protein levels after treatment with 25µM lycopene. Therefore, the results suggest that lycopene may be beneficial in delaying or preventing the progression of prostate disease.
Note
(type = degree)
M.S.
Note
(type = bibliography)
Includes bibliographical references (p. 55-60).
Subject
(ID = SUBJ1);
(authority = RUETD)
Topic
Food Science
Subject
(ID = SUBJ2);
(authority = ETD-LCSH)
Topic
Llycopene--Physiological effect
Subject
(ID = SUBJ3);
(authority = ETD-LCSH)
Topic
Cancer cells--Growth
Subject
(ID = SUBJ4);
(authority = ETD-LCSH)
Topic
Prostate--Cancer
RelatedItem
(type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier
(type = local)
rucore19991600001
Identifier
(type = hdl)
http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.16767
Identifier
ETD_519
Location
PhysicalLocation
(authority = marcorg);
(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T3RB7503
Genre
(authority = ExL-Esploro)
ETD graduate
Back to the top
Rights
RightsDeclaration
(AUTHORITY = GS);
(ID = rulibRdec0006)
The author owns the copyright to this work.
Copyright
Status
Copyright protected
Availability
Status
Open
AssociatedEntity
(AUTHORITY = rulib);
(ID = 1)
Name
Marynell Reyes
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
RightsEvent
(AUTHORITY = rulib);
(ID = 1)
Type
Permission or license
Detail
Non-exclusive ETD license
AssociatedObject
(AUTHORITY = rulib);
(ID = 1)
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Back to the top
Technical
Format
(TYPE = mime);
(VERSION = )
application/x-tar
FileSize
(UNIT = bytes)
1179648
Checksum
(METHOD = SHA1)
146e31807281bf7b51626ca968d9ddc561ba5d5b
ContentModel
ETD
CompressionScheme
other
OperatingSystem
(VERSION = 5.1)
windows xp
Format
(TYPE = mime);
(VERSION = NULL)
application/x-tar
Back to the top
Statistical Profile