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Physiology and molecular ecology of chemolithoautotrophic nitrate reducing bacteria at deep sea hydrothermal vents
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Title
Physiology and molecular ecology of chemolithoautotrophic nitrate reducing bacteria at deep sea hydrothermal vents
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Voordeckers
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James Walter
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James Walter Voordeckers
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author
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Vetriani
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Constantino
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Advisory Committee
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Constantino Vetriani
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chair
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Haggblom
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Max
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Advisory Committee
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Max Haggblom
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internal member
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Young
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Lily
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Advisory Committee
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Lily Young
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internal member
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Kerkhof
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Lee
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Advisory Committee
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Lee Kerkhof
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Rutgers University
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degree grantor
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Graduate School - New Brunswick
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theses
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2007
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2007
Language
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English
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electronic
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xiii, 115 pages
Abstract
At hydrothermal vent systems, the ability of microorganisms to use sulfur containing compounds for metabolic purposes has been long established while little is known regarding nitrogen metabolism. The objective of this thesis was to gain a better understanding of how microorganisms are involved in the cycling of nitrogen at deep sea hydrothermal vents through culture dependent and independent methods, isolation of novel nitrate reducing microorganisms, and phylogenetic surveys (16S rRNA gene, citrate lyase (aclA and aclB), and periplasmic nitrate reductase (napA)) of isolates and environmental samples. Sulfide, fluid, and bacterial filament samples from three separate hydrothermal vent sites (Rainbow, Logatchev, and Broken Spur) along the Mid Atlantic Ridge (MAR) and from 9° N on the East Pacific Rise (EPR) were used for isolation and phylogenetic surveys. Several novel autotrophic nitrate ammonifying bacterial strains belonging to the Epsilonproteobacteria were isolated with strain TB2 described as a new species, Caminibacter mediatlanticus. Phylogenetic surveys of the 16S rRNA gene, aclB, and napA showed the dominance of C. mediatlanticus related organisms at the Rainbow hydrothermal vent system indicating that we had successfully isolated an environmentally relevant organism. The environmental survey for napA was the first to be completed for deep sea hydrothermal vents. The phylogenetic survey of napA in reference organisms and environmental samples indicated that there is a wide diversity of Epsilonproteobacterial related sequences present at hydrothermal vents, with the greatest diversity seen within the mesophilic temperature range. It also appears that the periplasmic nitrate reductase gene (napA) is possibly being laterally transferred between members among the Epsilonproteobacteria.
Note
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Ph.D.
Note
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Includes bibliographical references (p. 102-114).
Subject
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Topic
Microbiology and Molecular Genetics
Subject
(ID = SUBJ2);
(authority = ETD-LCSH)
Topic
Deep-sea ecology
Subject
(ID = SUBJ3);
(authority = ETD-LCSH)
Topic
Hydrothermal vent ecology
Subject
(ID = SUBJ4);
(authority = ETD-LCSH)
Topic
Nitrogen--Metabolism
Subject
(ID = SUBJ5);
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Topic
Bacteria
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Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier
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rucore19991600001
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http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.16790
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ETD_329
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NjNbRU
Identifier
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doi:10.7282/T3KS6RZ7
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ETD doctoral
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Rights
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The author owns the copyright to this work.
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Copyright protected
Availability
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Open
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Name
James Voordeckers
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Affiliation
Rutgers University. Graduate School - New Brunswick
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Non-exclusive ETD license
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Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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