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The use of denaturing gradient gel electrophoresis in examining the species-specific influence of ectomycorrhizal fungi on selective bacteria enrichment in the mycorrhizosphere of Pinius rigida grown in a natural pine barrens habitat

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TitleInfo (displayLabel = Citation Title); (type = uniform)
Title
The use of denaturing gradient gel electrophoresis in examining the species-specific influence of ectomycorrhizal fungi on selective bacteria enrichment in the mycorrhizosphere of Pinius rigida grown in a natural pine barrens habitat
Name (ID = NAME001); (type = personal)
NamePart (type = family)
Ward
NamePart (type = given)
Rachel Jane
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Rachel Jane Ward
Role
RoleTerm (authority = RUETD)
author
Name (ID = NAME002); (type = personal)
NamePart (type = family)
Dighton
NamePart (type = given)
John
Affiliation
Advisory Committee
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John Dighton
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chair
Name (ID = NAME003); (type = personal)
NamePart (type = family)
Barkay
NamePart (type = given)
Tamar
Affiliation
Advisory Committee
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Tamar Barkay
Role
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co-chair
Name (ID = NAME004); (type = personal)
NamePart (type = family)
Vetriani
NamePart (type = given)
Costantino
Affiliation
Advisory Committee
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Costantino Vetriani
Role
RoleTerm (authority = RULIB)
internal member
Name (ID = NAME005); (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (ID = NAME006); (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (qualifier = exact)
2007
DateOther (qualifier = exact); (type = degree)
2007
Language
LanguageTerm
English
PhysicalDescription
Form (authority = marcform)
electronic
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application/pdf
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text/xml
Extent
x, 66 pages
Abstract
The study presented here describes the results of denaturing gradient gel electrophoresis (DGGE) used to detect bacterial diversity and selection in the ectomycorrhizasphere of Pinius rigida growing in a natural pine barrens habitat This study was the first to explore such a relationship in an environmental setting. Two study sites were chosen from the pine barrens in southern New Jersey, one from Rutgers Pinelands Field Station and one from Double Trouble State Park. Soils were from similar habitats and were similar in total phosphorous. The two sites were selected based on preliminary information that mercury content was high in the Double Trouble. However, upon further analysis, results showed that mercury was at a non-contaminant level in both sites. DGGE analysis of ectomycorrhiza fungi present on a root tip revealed that there were multiple species occurring where one morphotype was apparent from inspection through a dissecting microscope. DGGE analysis of ectomycorrhiza associated bacteria revealed a highly diverse community with little enrichment. While there was some enrichment noted on the bacterial DGGE profiles, this did not correspond to a species-specific link between ectomycorrhiza fungi and their associated bacteria. It is the conclusion of this study that the natural ectomycorrhizasphere is a highly complex environment, and that this complexity diffuses the influence of any one ectomycorrhizal species on the enrichement on bacteria present in the ectomycorrhizasphere.
Note (type = degree)
M.S.
Note (type = bibliography)
Includes bibliographical references (p. 64-66).
Subject (ID = SUBJ1); (authority = RUETD)
Topic
Ecology and Evolution
Subject (ID = SUBJ2); (authority = ETD-LCSH)
Topic
Ectomycorrhizal fungi
Subject (ID = SUBJ3); (authority = ETD-LCSH)
Topic
Soil fungi
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Identifier (type = hdl)
http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.16793
Identifier
ETD_544
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T3028RZG
Genre (authority = ExL-Esploro)
ETD graduate
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The author owns the copyright to this work.
Copyright
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Availability
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Open
AssociatedEntity (AUTHORITY = rulib); (ID = 1)
Name
Rachel Ward
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
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Detail
Non-exclusive ETD license
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Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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