Staff View
Candidate gene and microRNA expression studies of schizophrenia

Descriptive

TitleInfo (displayLabel = Citation Title); (type = uniform)
Title
Candidate gene and microRNA expression studies of schizophrenia
Name (ID = NAME001); (type = personal)
NamePart (type = family)
Bruse
NamePart (type = given)
Shannon E.
DisplayForm
Shannon E. Bruse
Role
RoleTerm (authority = RUETD)
author
Name (ID = NAME002); (type = personal)
NamePart (type = family)
Rongo
NamePart (type = given)
Christopher
Affiliation
Advisory Committee
DisplayForm
Christopher Rongo
Role
RoleTerm (authority = RULIB)
chair
Name (ID = NAME003); (type = personal)
NamePart (type = family)
Brzustowicz
NamePart (type = given)
Linda
Affiliation
Advisory Committee
DisplayForm
Linda Brzustowicz
Role
RoleTerm (authority = RULIB)
internal member
Name (ID = NAME004); (type = personal)
NamePart (type = family)
Firestein
NamePart (type = given)
Bonnie
Affiliation
Advisory Committee
DisplayForm
Bonnie Firestein
Role
RoleTerm (authority = RULIB)
internal member
Name (ID = NAME005); (type = personal)
NamePart (type = family)
Buyske
NamePart (type = given)
Steve
Affiliation
Advisory Committee
DisplayForm
Steve Buyske
Role
RoleTerm (authority = RULIB)
outside member
Name (ID = NAME006); (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (ID = NAME007); (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (qualifier = exact)
2008
DateOther (qualifier = exact); (type = degree)
2008-01
Language
LanguageTerm
English
PhysicalDescription
Form (authority = marcform)
electronic
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
viii, 115 pages
Note
Supplementary File: Supplementary Information 1 - Primer and Probe Sets
Abstract
Schizophrenia is a debilitating psychiatric disorder affecting 0.5-1% of the world's population. Schizophrenia has a significant genetic component, but the early search for specific genes has been slowed by a number of factors, including a lack of inexpensive genotyping methods suitable for targeted genetic studies. This work describes the development of an inexpensive multiplexed genotyping assay ideal for candidate gene studies. Furthermore, we describe application of this technology to the study of three schizophrenia candidate genes - SNAP-25, ZDHHC8, and DGCR8. Both SNAP-25 and ZDHHC8 are involved in glutamate signaling at the NMDA receptor, and dysfunction in glutamate signaling at the NMDA receptor is one of the leading theories of the etiology of schizophrenia. DGCR8 is an RNA-binding protein necessary for the processing of microRNAs, a class of small RNAs important in the development and maintenance of the mammalian central nervous system. Though we failed to find evidence of genetic association with ZDHHC8, we did identify a genetic association between schizophrenia and variants within both the SNAP-25 and DGCR8 genes. Our results with DGCR8 provide the first genetic link between the microRNA biogenesis pathway and a major neuropsychiatric disorder. Additionally, we performed quantitative microRNA expression profiling in post-mortem brain samples from patients with schizophrenia, bipolar disorder, and psychiatrically normal controls. We began with a pilot study comparing the expression of 158 microRNAs in the dorsolateral prefrontal cortex (BA9) of post-mortem brain tissue. Based on initial results, we assessed the expression of a number of specific microRNAs in a large, well characterized sample set from patients with schizophrenia (n=35), bipolar disorder (n=35), and psychiatrically normal controls (n=35). In the larger sample set, we observed overexpression of one microRNA, hsa-mir-372. This overexpression was observed in two separate brain regions from patients with both schizophrenia and bipolar disorder, though no differential expression of any microRNA tested was deemed statistically significant.
Note (type = degree)
Ph.D.
Note (type = bibliography)
Includes bibliographical references (p. 102-110).
Subject (ID = SUBJ1); (authority = RUETD)
Topic
Microbiology and Molecular Genetics
Subject (ID = SUBJ2); (authority = ETD-LCSH)
Topic
Schizophrenia--Genetic aspects
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Identifier (type = hdl)
http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.17103
Identifier
ETD_696
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T3V69JZH
Genre (authority = ExL-Esploro)
ETD doctoral
Back to the top

Rights

RightsDeclaration (AUTHORITY = GS); (ID = rulibRdec0006)
The author owns the copyright to this work.
Copyright
Status
Copyright protected
Availability
Status
Open
AssociatedEntity (AUTHORITY = rulib); (ID = 1)
Name
shannon bruse
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
RightsEvent (AUTHORITY = rulib); (ID = 1)
Type
Permission or license
Detail
Non-exclusive ETD license
AssociatedObject (AUTHORITY = rulib); (ID = 1)
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Back to the top

Technical

An error occurred while attempting to load this metadata section
Back to the top
Version 8.5.5
Rutgers University Libraries - Copyright ©2024