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Bioactives of Artemisia dracunculus L enhance cellular insulin signaling in primary human skeletal muscle culture

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TitleInfo
Title
Bioactives of Artemisia dracunculus L enhance cellular insulin signaling in primary human skeletal muscle culture
Name (type = personal)
NamePart (type = family)
Ribnicky
NamePart (type = given)
David M.
Affiliation
Biotechnology Center for Agriculture & the Environment, Rutgers University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (type = personal)
NamePart (type = family)
Cefalu
NamePart (type = given)
William T.
Affiliation
Pennington Biomedical Research Center, Louisiana State University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (type = personal)
NamePart (type = family)
Raskin
NamePart (type = given)
Ilya
Affiliation
Biotechnology Center for Agriculture & the Environment, Rutgers University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (type = personal)
NamePart (type = family)
Wang
NamePart (type = given)
Zhong Q.
Affiliation
Pennington Biomedical Research Center, Louisiana State University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (type = personal)
NamePart (type = family)
Zhang
NamePart (type = given)
Xian H.
Affiliation
Pennington Biomedical Research Center, Louisiana State University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (type = personal)
NamePart (type = family)
Yu
NamePart (type = given)
Yongmei
Affiliation
Pennington Biomedical Research Center, Louisiana State University
Role
RoleTerm (authority = marcrt); (type = text)
author
TypeOfResource
Text
Genre (authority = RULIB-FS)
articles
Genre (authority = NISO JAV)
Version of Record (VoR)
Genre (authority = ExL-Esploro)
Journal Article
OriginInfo
Publisher
Elsevier
DateIssued (encoding = w3cdtf); (keyDate = yes); (qualifier = exact)
2008
Language
LanguageTerm
English
PhysicalDescription
Form (authority = RULIB)
manuscript
InternetMediaType
application/pdf
Extent
7 pages
Abstract (type = abstract)
An alcoholic extract of Artemisia dracunculus L (PMI 5011) has been shown to decrease glucose and improve insulin levels in animal models, suggesting an ability to enhance insulin sensitivity. We sought to assess the cellular mechanism by which this botanical affects carbohydrate metabolism in primary human skeletal muscle culture. We measured basal and insulin-stimulated glucose uptake, glycogen accumulation, hosphoinositide 3 (PI-3) kinase activity, and Akt phosphorylation in primary skeletal muscle culture from subjects with type 2 diabetes mellitus incubated with or without various concentrations of PMI 5011. We also analyzed the abundance of insulin receptor signaling proteins, for example, IRS-1, IRS-2, and PI-3 kinase. Glucose uptake was significantly increased in the presence of increasing concentrations of PMI 5011. In addition, glycogen accumulation, observed to be decreased with increasing free fatty acid levels, was partially restored with PMI 5011. PMI 5011 treatment did not appear to significantly affect protein abundance for IRS-1, IRS-2, PI-3 kinase, Akt, insulin receptor, or Glut-4. However, PMI 5011 significantly decreased levels of a specific protein tyrosine phosphatase, that is, PTP1B. Time course studies confirmed that protein abundance of PTP1B decreases in the presence of PMI 5011. The cellular mechanism of action to explain the effects by which an alcoholic extract of A dracunculus L improves carbohydrate metabolism on a clinical level may be secondary to enhancing insulin receptor signaling and modulating levels of a specific protein tyrosine phosphatase, that is, PTP1B.
Note
Supported by NIH Grant P50AT002776-01 from the National Center for Complementary and Alternative Medicine (NCCAM) and the Office of Dietary Supplements (ODS), which funds the Botanical Research Center of Pennington Biomedical Research Center and The Biotech Center of Rutgers University.
Note (type = grantNumber)
NIH P50 AT002776-01; by William Cefalu
Note
The published version of this article is available at: http://www.metabolismjournal.com/
Subject (authority = Other)
Topic
Artemisia dracunculus L
Subject (authority = LCSH)
Topic
Insulin resistance
Subject (authority = LCSH)
Topic
Glucose--Metabolism
Subject (authority = Other)
Topic
Human skeletal muscle culture
RelatedItem (type = host)
TitleInfo
Title
Ribnicky, David
Identifier (type = local)
rucore00000001163
Identifier (type = hdl)
http://hdl.rutgers.edu/1782.2/rucore00000001163.Article.17201
Identifier (type = doi)
doi:10.7282/T3N29VBX
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Extension
DescriptiveEvent
Type
Citation
DateTime (encoding = w3cdtf); (qualifier = approximate)
2008
AssociatedObject
Type
Journal
Relationship
Has part
Name
Metabolism Clinical and Experimental
Identifier (type = volume and issue)
57(suppl 1)
Reference (type = digital)
http://dx.doi.org/10.1016/j.metabol.2008.04.003
Detail
S58-S64
Extension
DescriptiveEvent
Type
Grant award
AssociatedEntity
Role
Funder
Name
National Institutes of Health
AssociatedEntity
Role
Originator
Name
William Cefalu
AssociatedObject
Type
Grant number
Name
NIH P50 AT002776-01
Name (authority = RutgersOrg-School); (type = corporate)
NamePart
Biotechnology Center for Agriculture & the Environment
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RightsDeclaration (AUTHORITY = FS); (ID = rulibRdec0004)
Copyright for scholarly resources published in RUcore is retained by the copyright holder. By virtue of its appearance in this open access medium, you are free to use this resource, with proper attribution, in educational and other non-commercial settings. Other uses, such as reproduction or republication, may require the permission of the copyright holder.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
RightsHolder (ID = 1)
Name (TYPE = corporate)
CorporateName
Elsevier Ltd.
RightsEvent (AUTHORITY = rulib); (ID = 1)
Type
Permission or license
AssociatedObject (AUTHORITY = rulib); (ID = 1)
Type
License
Name
David Ribnicky
Detail
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I further represent and warrant that I have obtained all necessary rights to permit Rutgers to reproduce and distribute the Work and that any third-party owned content is clearly identified and acknowledged within the Work.
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ContentModel
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