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Development of controlled release antimicrobial films from low methoxyl pectin
Descriptive
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Title
Development of controlled release antimicrobial films from low methoxyl pectin
Name
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Marathe
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Rohini
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Rohini Marathe
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author
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Yam
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Kit
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Advisory Committee
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Kit Yam
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chair
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Chikindas
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Mikhael
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Advisory Committee
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Mikhael Chikindas
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internal member
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Huang
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Qingrong
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Advisory Committee
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Qingrong Huang
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dissertation committee member
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Schaich
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Karen
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Advisory Committee
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Karen Schaich
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internal member
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Rutgers University
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degree grantor
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Graduate School - New Brunswick
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school
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Text
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theses
OriginInfo
DateCreated
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2008
DateOther
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2008-01
Language
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English
PhysicalDescription
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electronic
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application/pdf
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text/xml
Extent
ix, 80 pages
Abstract
Biopolymer-based controlled release antimicrobial packaging is an innovative packaging that aids in controlled replenishment of antimicrobial compound at the food surface, where it is required the most. Various biopolymers have been evaluated for controlled release purposes; focus being mainly on their antimicrobial effectiveness. However, low methoxyl pectin, with the potential to control release of active compounds by forming different degrees of crosslinking, has not been exploited in antimicrobial packaging and there is a lack of understanding of different variables affecting the release properties of active compounds from pectin films.
The main objective of this thesis was to identify the key variables involved in the development of pectin-based antimicrobial films by evaluation of the effect of different variables such as composition variables (size of active compound and degree of calcium crosslinking), process variables (pH of pectin, method of crosslinking), and environmental variables on the release of antimicrobial compounds, especially nisin. A secondary objective was to demonstrate the concept that release of active compounds could be altered by varying the degree of calcium crosslinking within the pectin matrix.
Antimicrobial compounds such as sodium benzoate, potassium sorbate and nisin were chosen based on their different physical and chemical properties. Coomassie blue dye was used as a model compound for proof of concept. Films were produced from LM pectin by varying the DE of pectin and calcium concentration using the solution casting method. Release of antimicrobial compounds into water as a food simulant was measured by UV/Visible spectrophotometry or HPLC or agar diffusion assay, depending on the antimicrobial compound being evaluated.
Degree of calcium crosslinking, pH of pectin slurry and method of calcium crosslinking were identified as the key variables controlling release of nisin from pectin films. Electrostatic attraction between pectin and nisin at pH above pKa of pectin was not sufficiently strong to prevent the release of nisin. Release studies with dye-containing pectin films demonstrated that variable release rates could be obtained by altering the degree of calcium crosslinking.
The main objective of this thesis was to identify the key variables involved in the development of pectin-based antimicrobial films by evaluation of the effect of different variables such as composition variables (size of active compound and degree of calcium crosslinking), process variables (pH of pectin, method of crosslinking), and environmental variables on the release of antimicrobial compounds, especially nisin. A secondary objective was to demonstrate the concept that release of active compounds could be altered by varying the degree of calcium crosslinking within the pectin matrix.
Antimicrobial compounds such as sodium benzoate, potassium sorbate and nisin were chosen based on their different physical and chemical properties. Coomassie blue dye was used as a model compound for proof of concept. Films were produced from LM pectin by varying the DE of pectin and calcium concentration using the solution casting method. Release of antimicrobial compounds into water as a food simulant was measured by UV/Visible spectrophotometry or HPLC or agar diffusion assay, depending on the antimicrobial compound being evaluated.
Degree of calcium crosslinking, pH of pectin slurry and method of calcium crosslinking were identified as the key variables controlling release of nisin from pectin films. Electrostatic attraction between pectin and nisin at pH above pKa of pectin was not sufficiently strong to prevent the release of nisin. Release studies with dye-containing pectin films demonstrated that variable release rates could be obtained by altering the degree of calcium crosslinking.
Note
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M.S.
Note
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Includes bibliographical references (p. 73-80).
Subject
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Topic
Food Science
Subject
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Topic
Food contamination--Prevention
Subject
(ID = SUBJ3);
(authority = ETD-LCSH)
Topic
Food--Packaging
Subject
(ID = SUBJ4);
(authority = ETD-LCSH)
Topic
Anti-infective agents
Subject
(ID = SUBJ5);
(authority = ETD-LCSH)
Topic
Plastic films
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TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier
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rucore19991600001
Identifier
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http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.17160
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ETD_719
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NjNbRU
Identifier
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doi:10.7282/T3416XFZ
Genre
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ETD graduate
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The author owns the copyright to this work.
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Copyright protected
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Open
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Name
Rohini Marathe
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Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
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Non-exclusive ETD license
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Author Agreement License
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I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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