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Selective plant growth using D-amino acids
Descriptive
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Title
Selective plant growth using D-amino acids
Name
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NamePart
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Bosacchi
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Massimo
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Massimo Bosacchi
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author
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Kerstetter
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Randall
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Advisory Committee
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Randall Kerstetter
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chair
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Maliga
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Pal
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Advisory Committee
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Pal Maliga
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internal member
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Frenkel
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Chaim
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Advisory Committee
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Chaim Frenkel
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internal member
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Rutgers University
Role
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degree grantor
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Graduate School - New Brunswick
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school
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Text
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theses
OriginInfo
DateCreated
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2008
DateOther
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2008-05
Language
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English
PhysicalDescription
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electronic
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application/pdf
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text/xml
Extent
iv, 46 pages
Abstract
Selectable marker genes are essential for the efficient selection of transgenic plants. Heterologous genes used as markers typically encode enzymes that neutralize a toxic compound, allowing for positive selection of plants containing the marker. We report on the efficacy of a novel marker gene system that exploits the varying phytotoxicity of D-amino acids and their oxidative deamination products. Our investigation of a putative DAAO gene from Schizosaccharomyces pombe reveals sufficient oxidative deaminase activity to confer D-alanine and D-serine tolerance to transgenic Arabidopsis thaliana plants carrying the heterologous spDAAO gene. We have demonstrated that the spDAAO, when used in conjunction with D-alanine, allows for the positive selection of primary transformants at levels comparable to hygromycin. Additionally, our selection scheme carries the potential for negative selection in the presence of different selection substrates, such as D-valine or D-isoleucine. This attribute, known as conditional selection would provide particular utility to applications involving site-specific recombinase mediated marker gene removal.
Note
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M.S.
Note
(type = bibliography)
Includes bibliographical references (p. 42-46).
Subject
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Topic
Plant Biology
Subject
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(authority = ETD-LCSH)
Topic
Growth (Plants)
Subject
(ID = SUBJ3);
(authority = ETD-LCSH)
Topic
Amino acids
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TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier
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rucore19991600001
Identifier
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http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.17279
Identifier
ETD_833
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(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T3P84C8Q
Genre
(authority = ExL-Esploro)
ETD graduate
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Rights
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The author owns the copyright to this work.
Copyright
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Copyright protected
Availability
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Open
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Name
Massimo Bosacchi
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
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Permission or license
Detail
Non-exclusive ETD license
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License
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Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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Technical
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964096
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