Brain-specific microRNAs induce neurogenesis through indirect regulation of Mef2C activity
Name (ID = NAME001); (type = personal)
NamePart (type = family)
Goff
NamePart (type = given)
Loyal Andrew
DisplayForm
Loyal Andrew Goff
Role
RoleTerm (authority = RUETD)
author
Name (ID = NAME002); (type = personal)
NamePart (type = family)
Hart
NamePart (type = given)
Ronald
Affiliation
Advisory Committee
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Ronald P Hart
Role
RoleTerm (authority = RULIB)
chair
Name (ID = NAME003); (type = personal)
NamePart (type = family)
Padgett
NamePart (type = given)
Richard
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Advisory Committee
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Richard W Padgett
Role
RoleTerm (authority = RULIB)
internal member
Name (ID = NAME004); (type = personal)
NamePart (type = family)
Grumet
NamePart (type = given)
Martin
Affiliation
Advisory Committee
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Martin Grumet
Role
RoleTerm (authority = RULIB)
internal member
Name (ID = NAME005); (type = personal)
NamePart (type = family)
Jornsten
NamePart (type = given)
Rebecka
Affiliation
Advisory Committee
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Rebecka Jornsten
Role
RoleTerm (authority = RULIB)
internal member
Name (ID = NAME006); (type = personal)
NamePart (type = family)
Getts
NamePart (type = given)
Robert
Affiliation
Advisory Committee
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Robert Getts
Role
RoleTerm (authority = RULIB)
outside member
Name (ID = NAME007); (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (ID = NAME008); (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (qualifier = exact)
2008
DateOther (qualifier = exact); (type = degree)
2008-05
Language
LanguageTerm
English
PhysicalDescription
Form (authority = marcform)
electronic
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
xix, 111 pages
Abstract
MicroRNAs represent a group of functional non-coding RNAs (ncRNA) with a role in both translational repression and/or RNAi-mediated degradation of specific target mRNAs. Reports indicate that microRNA regulation plays an important role in numerous cellular processes. We have identified microRNAs regulated during induction of neurogenesis in an in vitro model, and have correlated the expression of these specific microRNAs to regulated mRNAs with the goal of describing potential functional interactions between the two sets of molecules. We demonstrate the active role these regulated microRNAs play in inducing a neuronal phenotype in uncommitted neural precursor cells. As a result of this analysis, as well as additional studies of regulated mRNA transcripts during neurogenesis, we have identified a regulated transcription factor, Mef2C, which is chromosomally adjacent to one of the regulated microRNAs. The unexpected appearance of this transcription factor and its relatively unexplored role in neural differentiation suggest that Mef2C may play a role in this cellular process. This single genomic locus containing an induced microRNA involved in neuronal fate determination, as well as Mef2C, is transcriptionally regulated during neuronal cell specification. In addition, HDAC4, a known repressor of Mef2C activity is validated as a target for miR-9 mediated post-transcriptional repression. Described here is a putative network of interactions that arise from transcriptional activation of this locus, with the end result contributing to the induction of a neuronal phenotype.
Note (type = degree)
Ph.D.
Note (type = bibliography)
Includes bibliographical references (p. 96-110).
Subject (ID = SUBJ1); (authority = RUETD)
Topic
Cell and Developmental Biology
Subject (ID = SUBJ2); (authority = ETD-LCSH)
Topic
Non-coding RNA
Subject (ID = SUBJ3); (authority = ETD-LCSH)
Topic
Developmental neurobiology
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T37P8ZRN
Genre (authority = ExL-Esploro)
ETD doctoral
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Open
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Name
Loyal Goff
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
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Non-exclusive ETD license
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I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.