Staff View
Molecular analysis of microbial 16S rRNA, mcrA, dsrAB and pmoA genes from deep-sea hydrothermal vent and cold seep sites

Descriptive

TitleInfo (displayLabel = Citation Title); (type = uniform)
Title
Molecular analysis of microbial 16S rRNA, mcrA, dsrAB and pmoA genes from deep-sea hydrothermal vent and cold seep sites
Name (ID = NAME001); (type = personal)
NamePart (type = family)
Reed
NamePart (type = given)
Andrew Jay
DisplayForm
Andrew Jay Reed
Role
RoleTerm (authority = RUETD)
author
Name (ID = NAME002); (type = personal)
NamePart (type = family)
Lutz
NamePart (type = given)
Richard
Affiliation
Advisory Committee
DisplayForm
Richard A Lutz
Role
RoleTerm (authority = RULIB)
chair
Name (ID = NAME003); (type = personal)
NamePart (type = family)
Vetriani
NamePart (type = given)
Costantino
Affiliation
Advisory Committee
DisplayForm
Costantino Vetriani
Role
RoleTerm (authority = RULIB)
internal member
Name (ID = NAME004); (type = personal)
NamePart (type = family)
Kennish
NamePart (type = given)
Michael
Affiliation
Advisory Committee
DisplayForm
Michael J Kennish
Role
RoleTerm (authority = RULIB)
internal member
Name (ID = NAME005); (type = personal)
NamePart (type = family)
Shank
NamePart (type = given)
Timothy
Affiliation
Advisory Committee
DisplayForm
Timothy M Shank
Role
RoleTerm (authority = RULIB)
outside member
Name (ID = NAME006); (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (ID = NAME007); (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (qualifier = exact)
2008
DateOther (qualifier = exact); (type = degree)
2008-10
Language
LanguageTerm
English
PhysicalDescription
Form (authority = marcform)
electronic
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
xii, 81 pages
Abstract
Methane and sulfide are primary sources of energy supporting the dense macrobiological communities often found in cold seep areas and both free-living and symbiotic microorganisms depend on energy provided by the sharp gradient of sulfide and the reservoir of methane in the underlying sediments. In deep-sea hydrothermal vent areas, sulfide is typically the primary source of energy for microbial primary production. To investigate microbial populations and community structure in these areas, microbial communities from both deep-sea hydrothermal vent (Rainbow and Logatchev hydrothermal vent fields, Mid-Atlantic Ridge, Atlantic Ocean; 9°N hydrothermal vent area, East Pacific Rise, Pacific Ocean) and cold seep areas (Blake Ridge, western Atlantic Ocean; Florida Escarpment, Gulf of Mexico) were evaluated by molecular phylogenetic analysis of 16S rRNA, mcrA, dsrAB and pmoA gene sequences. Twenty-one clone libraries were obtained using DNA from friable vent chimney material (hydrothermal vent areas) and from vertically subsampled (top, middle and bottom) sediment core samples (cold seep areas), screened by RFLP and sequenced.
At the Florida Escarpment, phylogenetic analysis of bacterial 16S rDNA suggests the dominance of ε-Proteobacteria in the top zone, the ε- δ- and γ-Proteobacteria in the middle zone and the δ-Proteobacteria in the bottom zone of the core. Archaeal diversity was low throughout, but increased with depth. Cold seep mcrA sequences were distributed among the ANME-2c, -2d and -2e groups. Clone library dsrAB sequences grouped primarily within the orders Desulfobacteriales, Syntrophobacteriales and the gram-positive order Clostridales. Clone library pmoA sequences grouped among the Type I methanotrophs (γ-proteobacteria) within the order Methylococcales. Most sequences recovered represented as-yet-uncultivated phylotypes distinct from any other cultivated or environmental clones. In addition, this investigation presents for the first time environmental mcrA sequences detected from chimney samples collected from a bare basalt, high-temperature deep-sea hydrothermal vent area that are related to ANME-2e sequences previously shown to be associated with the anaerobic oxidation of methane.
Note (type = degree)
Ph.D.
Note (type = bibliography)
Includes bibliographical references (p. 69-81).
Subject (ID = SUBJ1); (authority = RUETD)
Topic
Ecology and Evolution
Subject (ID = SUBJ2); (authority = ETD-LCSH)
Topic
Hydrothermal vent ecology
Subject (ID = SUBJ3); (authority = ETD-LCSH)
Topic
Microbial genetics
Subject (ID = SUBJ4); (authority = ETD-LCSH)
Topic
Microbial ecology
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Identifier (type = hdl)
http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.17554
Identifier
ETD_1302
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T3DJ5FXQ
Genre (authority = ExL-Esploro)
ETD doctoral
Back to the top

Rights

RightsDeclaration (AUTHORITY = GS); (ID = rulibRdec0006)
The author owns the copyright to this work.
Copyright
Status
Copyright protected
Availability
Status
Open
AssociatedEntity (AUTHORITY = rulib); (ID = 1)
Name
Andrew Reed
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
RightsEvent (AUTHORITY = rulib); (ID = 1)
Type
Permission or license
Detail
Non-exclusive ETD license
AssociatedObject (AUTHORITY = rulib); (ID = 1)
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Back to the top

Technical

Format (TYPE = mime); (VERSION = )
application/x-tar
FileSize (UNIT = bytes)
1377792
Checksum (METHOD = SHA1)
72a9e667b45105304fef0a3b420e67099c6d0b37
ContentModel
ETD
CompressionScheme
other
OperatingSystem (VERSION = 5.1)
windows xp
Format (TYPE = mime); (VERSION = NULL)
application/x-tar
Back to the top
Version 8.5.5
Rutgers University Libraries - Copyright ©2024