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Target(s) of transcriptional regulators ppGpp and DksA

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TypeOfResource
Text
TitleInfo (ID = T-1)
Title
Target(s) of transcriptional regulators ppGpp and DksA
SubTitle
PartName
PartNumber
NonSort
Identifier
ETD_1474
Identifier (type = hdl)
http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000051014
Language (objectPart = )
LanguageTerm (authority = ISO639-2); (type = code)
eng
Genre (authority = marcgt)
theses
Subject (ID = SBJ-1); (authority = RUETD)
Topic
Chemical and Biochemical Engineering
Subject (ID = SBJ-2); (authority = ETD-LCSH)
Topic
Genetic regulation
Subject (ID = SBJ-3); (authority = ETD-LCSH)
Topic
DNA-binding proteins
Abstract
Bacterial RNA polymerase is a highly regulated enzyme, altering the metabolic and functional state of bacteria in response to their environment. rRNA synthesis is a major metabolic expense and is, thus, highly regulated. One such response is the ppGpp-induced stringent response which changes the metabolic state of the cell, particularly inhibiting rRNA synthesis. ppGpp, guanosine tetraphosphate, is produced rapidly during amino acid starvation. ppGpp acts along with an accessory molecule, a protein DksA. The mechanism and binding site of ppGpp/DksA remains unclear and disputed. The objective of this study is to determine the binding site of ppGpp and DksA and understand the mechanism by which they regulate the cell's metabolic state. The strategy is to randomly mutagenize RNAP genes, specifically rpoC which codes for β' subunit, and perform genetic screens to identify mutants resistant to toxic effects of ppGpp/DksA. The mutants were isolated, sequenced, tested and characterized by in vivo and in vitro assays. The mutants isolated in this study belong to two distinct clusters: the switch region, which controls the opening and closing of active center clamp allowing dsDNA entry, and the secondary channel, which is the channel through which NTP substrates enter. The mutants in the switch region could imply a conformational determinant for ppGpp/DksA. The secondary channel mutants could be binding/conformational determinants for ppGpp/DksA. The in vitro results indicate that two mutants in the secondary channel interact with DksA which could confirm DksA?s putative binding site.
PhysicalDescription
Form (authority = gmd)
electronic resource
Extent
viii, 59 p. : ill.
InternetMediaType
application/pdf
InternetMediaType
text/xml
Note (type = degree)
M.S.
Note (type = bibliography)
Includes bibliographical references (p. 55-58)
Note (type = statement of responsibility)
by Nimish Gupta
Name (ID = NAME-1); (type = personal)
NamePart (type = family)
Gupta
NamePart (type = given)
Nimish
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author
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Nimish Gupta
Name (ID = NAME-2); (type = personal)
NamePart (type = family)
Roth
NamePart (type = given)
Charles
Role
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chair
Affiliation
Advisory Committee
DisplayForm
Charles M Roth
Name (ID = NAME-3); (type = personal)
NamePart (type = family)
Ebright
NamePart (type = given)
Richard
Role
RoleTerm (authority = RULIB); (type = )
co-chair
Affiliation
Advisory Committee
DisplayForm
Richard H Ebright
Name (ID = NAME-4); (type = personal)
NamePart (type = family)
Pedersen
NamePart (type = given)
Henrik
Role
RoleTerm (authority = RULIB); (type = )
internal member
Affiliation
Advisory Committee
DisplayForm
Henrik Pedersen
Name (ID = NAME-1); (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB); (type = )
degree grantor
Name (ID = NAME-2); (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB); (type = )
school
OriginInfo
DateCreated (point = ); (qualifier = exact)
2009
DateOther (qualifier = exact); (type = degree)
2009-01
Location
PhysicalLocation (authority = marcorg)
NjNbRU
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Identifier (type = doi)
doi:10.7282/T3VD6ZQ8
Genre (authority = ExL-Esploro)
ETD graduate
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The author owns the copyright to this work.
Copyright
Status
Copyright protected
Availability
Status
Open
RightsEvent (AUTHORITY = rulib); (ID = 1)
Type
Permission or license
Detail
Non-exclusive ETD license
AssociatedObject (AUTHORITY = rulib); (ID = 1)
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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ETD
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application/pdf
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application/x-tar
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2007040
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