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Creation of a framework genetic linkage map of colonial bentgrass and the identification of genomic regions associated with dollar spot resistance.
Descriptive
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Text
TitleInfo
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Title
Creation of a framework genetic linkage map of colonial bentgrass and the identification of genomic regions associated with dollar spot resistance.
Identifier
ETD_1369
Identifier
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http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000051065
Language
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eng
Genre
(authority = marcgt)
theses
Subject
(ID = SBJ-1);
(authority = RUETD)
Topic
Plant Biology
Subject
(ID = SBJ-1);
(authority = ETD-LCSH)
Topic
Creeping bentgrass--Diseases and pests
Subject
(ID = SBJ-1);
(authority = ETD-LCSH)
Topic
Turfgrasses--Diseases and pests
Abstract
Creeping bentgrass (Agrostis stolonifera L. 2n=4x=28, A2A2A3A3) is a cool season turfgrass known for its lateral growth habit, fine texture and ability to tolerate low mowing heights. One of the biggest maintenance problems for creeping bentgrass is the fungal disease dollar spot caused by Sclerotinia homoeocarpa. Currently the disease is controlled through heavy fungicide use but a more practical long term approach lies through the development of resistant cultivars. Colonial bentgrass (A. capillaris L. 2n=4x=28, A1A1A2A2) is a species related to creeping bentgrass which is resistant to dollar spot. We have generated fertile creeping x colonial bentgrass interspecific hybrids. One plant, designated TH15, was backcrossed with a different creeping plant to generate a mapping population which segregated for dollar spot resistance. The objective of this thesis was to determine which areas of the colonial bentgrass genome are associated with dollar spot resistance. To do this we used the hybrid backcross population to generate a framework genetic linkage map of colonial bentgrass. We created and annotated EST libraries for both creeping and colonial bentgrass and used these libraries as a resource for mapping genes. A new approach to marker development, termed dideoxy polymorphism scanning, was developed to efficiently map genes on the colonial bentgrass map. The colonial bentgrass linkage map contains 212 AFLPs and 110 gene based markers and totals 1157 cM.
By comparing phenotypic data obtained from field trails we identified regions potentially associated with dollar spot resistance in colonial bentgrass. The segregation of resistance in the backcross population suggests a three gene recessive epistasis model for dollar spot resistance in colonial bentgrass. By comparing genotypes we identified colonial bentgrass loci on groups 2A1 and 3A1 that are common in all resistant individuals. We hypothesize that genes related to dollar spot resistance may lie on these groups. Other resistant backcross individuals not included in the mapping population also shared these loci proportions significantly higher then expected by random chance (p < 0.05). This lends additional support to our model of dollar spot resistance in colonial bentgrass.
By comparing phenotypic data obtained from field trails we identified regions potentially associated with dollar spot resistance in colonial bentgrass. The segregation of resistance in the backcross population suggests a three gene recessive epistasis model for dollar spot resistance in colonial bentgrass. By comparing genotypes we identified colonial bentgrass loci on groups 2A1 and 3A1 that are common in all resistant individuals. We hypothesize that genes related to dollar spot resistance may lie on these groups. Other resistant backcross individuals not included in the mapping population also shared these loci proportions significantly higher then expected by random chance (p < 0.05). This lends additional support to our model of dollar spot resistance in colonial bentgrass.
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electronic resource
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x, 157 p. : ill.
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Note
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Ph.D.
Note
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Includes bibliographical references (p. 144-156)
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by David Rotter
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Rotter
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David
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David Rotter
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Belanger
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Faith
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chair
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Advisory Committee
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Faith C Belanger
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(type = family)
Bonos
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Stacy
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internal member
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Advisory Committee
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Stacy A Bonos
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Goffreda
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Joseph
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Advisory Committee
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Joseph C Goffreda
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Zilinskas
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Barbara
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Advisory Committee
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Barbara A Zilinskas
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Zylstra
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Gerben
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outside member
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Advisory Committee
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Gerben J Zylstra
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Rutgers University
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degree grantor
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Graduate School - New Brunswick
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school
OriginInfo
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2009
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2009-01
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NjNbRU
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Title
Rutgers University Electronic Theses and Dissertations
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ETD
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TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier
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rucore19991600001
Identifier
(type = doi)
doi:10.7282/T33T9HG6
Genre
(authority = ExL-Esploro)
ETD doctoral
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The author owns the copyright to this work.
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Open
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Non-exclusive ETD license
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Author Agreement License
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I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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