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Regulation of Psc-Su(z)2 genes in drosophila melanogaster
Descriptive
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Text
TitleInfo
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Title
Regulation of Psc-Su(z)2 genes in drosophila melanogaster
Identifier
ETD_808
Identifier
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http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000051094
Language
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eng
Genre
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theses
Subject
(ID = SBJ-1);
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Topic
Biochemistry
Subject
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(authority = ETD-LCSH)
Topic
Drosophila melanogaster--Genetics
Abstract
The Psc and Su(z)2 genes belong to the Polycomb Group (PcG) and Psc itself is one of the core components of the PRC1 repressive complex and genetic evidence suggests that it is autoregulated by PcG mechanisms. Recently, the Psc-Su(z)2 region was also found to contain several putative Polycomb Response Elements that bind PcG proteins while the entire region is enriched for H3K27me3. Current model of PcG mechanism is all-or- none silencing paradigm derived from its role in homeotic gene regulation. However, it is likely that PcG regulation at many other target genes functions by down-regulating rather than silencing expression. This is certainly the case for PcG genes like Psc and Su(z)2. To understand how PcG mechanisms down-regulate a target gene, the nature of the PcG binding sites in the Psc-Su(z)2 region and their repressive effects were analyzed, using reporter gene construct. There are at least two functional PREs that can silence a reporter gene in a PcG-dependent manner and one of them can also show anti-silencing activity, depending on the chromosomal context. In addition, we found a down-regulation module in the vicinity of Psc promoter, whose effect is insensitive to the dosage of PcG genes. Probably, several different regulatory elements might be cooperative to down-regulate Psc-Su(z)2 genes by PcG mechanism. I have also generated small deletions that remove one such binding peak. Deletion of one of the Psc-Su(z)2 PREs increases the expression level of Psc and Su(z)2 by 2-3 fold at late embryonic stage. Perhaps the increased expression of PSC can partially compensate by binding to the other PREs in the Psc-Su(z)2 locus. On the contrary, the expressions of CG13323 and CG13324 genes behind of the PRE are decreased by 20 to 50 fold during entire embryonic stages. Also, the chromatin IP experiment showed that the loss of this fragment extends the domain of H3K27me3 around 10kb further, to a region that includes both of CG13323 and CG13324 transcripts. So, the fragment removed in both deletions may not possess only PRE but also a transcriptional enhancer for downstream genes or boundary element to block the PcG silencing.
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electronic resource
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xi, 100 p. : ill.
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Note
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Ph.D.
Note
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Includes bibliographical references (p. 92-99)
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by Sung-Yeon Park
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Park
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Sung-Yeon
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Sung-Yeon Park
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Steward
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Ruth
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chair
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Advisory Committee
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Ruth Steward
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Barth
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internal member
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Advisory Committee
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Barth Grant
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Pirrotta
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Vincenzo
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internal member
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Advisory Committee
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Vincenzo Pirrotta
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Mckim
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Kim
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outside member
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Advisory Committee
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Kim Mckim
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Rutgers University
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degree grantor
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Graduate School - New Brunswick
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school
OriginInfo
DateCreated
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2008
DateOther
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2008-05
Place
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xx
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Title
Rutgers University Electronic Theses and Dissertations
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ETD
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Title
Graduate School - New Brunswick Electronic Theses and Dissertations
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rucore19991600001
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(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T37P8ZMV
Genre
(authority = ExL-Esploro)
ETD doctoral
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Rights
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The author owns the copyright to this work.
Copyright
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Copyright protected
Availability
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Open
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Detail
Non-exclusive ETD license
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Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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4433920
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