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Embryonic stem cell-derived populations retain their tumorigenic potential

Descriptive

TypeOfResource
Text
TitleInfo (ID = T-1)
Title
Embryonic stem cell-derived populations retain their tumorigenic potential
Identifier
ETD_1604
Identifier (type = hdl)
http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000051170
Language
LanguageTerm (authority = ISO639-2); (type = code)
eng
Genre (authority = marcgt)
theses
Subject (ID = SBJ-1)
Name (authority = LC-NAF)
Subject (ID = SBJ-1); (authority = RUETD)
Topic
Cell and Developmental Biology
Subject (ID = SBJ-1); (authority = ETD-LCSH)
Topic
Embryonic stem cells--Therapeutic use
Abstract
Multiple sclerosis (MS) is a neurodegenerative disorder, which results in the death of oligodendrocytes, the myelinating cells of the central nervous system (CNS). Although drug therapies can abate the progression of MS, no treatment has reported significant repair of lost myelin. One possible solution would be to use an embryonic stem cell therapy to derive oligodendrocytes. However, ESC studies have stressed the need for have a homogenous cell population, because ESCs produce teratomas, benign embryonic tumors consisting of all three tissue layers. In this thesis, mouse ESCs were differentiated to oligodendrocytes using a six-step protocol. At each stage of the differentiation protocol, we asked whether teratogenic ESCs persisted within the differentiated cell population. If ESCs persisted, the cell population would have the ability to generate embryoid bodies (EBs). It was found that at each stage of differentiation, we were able to generate EB-like bodies. However, the frequency of EB formation decreased as differentiation progressed indicating a progressive reduction in the number of ESCs. The differentiated cell populations were characterized by immunocytochemistry and RT-PCR and compared and contrasted to starting ESC populations. We conclude that undifferentiated ESCs remain within the heterogeneous population of cells. Therefore, a possible cell therapy must have all ESCs removed from the final differentiated cell population.
PhysicalDescription
Form (authority = gmd)
electronic resource
Extent
vii, 40 p. : ill.
InternetMediaType
application/pdf
InternetMediaType
text/xml
Note (type = degree)
M.S.
Note (type = bibliography)
Includes bibliographical references (p. 37-40)
Note (type = statement of responsibility)
by Marisa Apicella
Name (ID = NAME-1); (type = personal)
NamePart (type = family)
Apicella
NamePart (type = given)
Marisa
NamePart (type = date)
1982
Role
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author
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Marisa Apicella
Name (ID = NAME-2); (type = personal)
NamePart (type = family)
Mckinnon
NamePart (type = given)
Randall
Role
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chair
Affiliation
Advisory Committee
DisplayForm
Randall Mckinnon
Name (ID = NAME-3); (type = personal)
NamePart (type = family)
Martin
NamePart (type = given)
Charles
Role
RoleTerm (authority = RULIB); (type = )
internal member
Affiliation
Advisory Committee
DisplayForm
Charles E Martin
Name (ID = NAME-4); (type = personal)
NamePart (type = family)
Habas
NamePart (type = given)
Raymond
Role
RoleTerm (authority = RULIB); (type = )
internal member
Affiliation
Advisory Committee
DisplayForm
Raymond Habas
Name (ID = NAME-1); (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB); (type = )
degree grantor
Name (ID = NAME-2); (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB); (type = )
school
OriginInfo
DateCreated (point = ); (qualifier = exact)
2009
DateOther (qualifier = exact); (type = degree)
2009-05
Place
PlaceTerm (type = code)
xx
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T3DF6RGZ
Genre (authority = ExL-Esploro)
ETD graduate
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RightsDeclaration (AUTHORITY = GS); (ID = rulibRdec0006)
The author owns the copyright to this work.
Copyright
Status
Copyright protected
Availability
Status
Open
RightsEvent (AUTHORITY = rulib); (ID = 1)
Type
Permission or license
Detail
Non-exclusive ETD license
AssociatedObject (AUTHORITY = rulib); (ID = 1)
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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Technical

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ETD
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application/pdf
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application/x-tar
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2867200
Checksum (METHOD = SHA1)
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