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Development and application of quantitative bioaerosol analysis method using PCR

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TypeOfResource
Text
TitleInfo (ID = T-1)
Title
Development and application of quantitative bioaerosol analysis method using PCR
Identifier
ETD_1649
Identifier (type = hdl)
http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000051173
Language
LanguageTerm (authority = ISO639-2); (type = code)
eng
Genre (authority = marcgt)
theses
Subject (ID = SBJ-1); (authority = RUETD)
Topic
Environmental Sciences
Subject (ID = SBJ-1); (authority = ETD-LCSH)
Topic
Pathogenic microorganisms--Detection
Subject (ID = SBJ-1); (authority = ETD-LCSH)
Topic
Aerosols
Subject (ID = SBJ-1); (authority = ETD-LCSH)
Topic
Air--Microbiology
Abstract
The presence of harmful airborne particulate matter of biological origin has been associated with variety of negative health effects. In addition, there is a real treat of malliciouse release of hazardouse bioaerosol to public sectors. To protect the population at risk from bioaerosol exposure, an effective bioaerosol detection system is urgently needed that enables a rapid and accurate bioaerosol sampling, identification, and quantification in air samples. As an effective bioaerosol monitorin system requires both an effective air sampling device and rapid sample analysis technique with high sensitivity, the performance of RCS High Flow was investigated with culture-based quantification technique in Chapter 1. The Results showed that the test sampler would collect more than 80 % of common fungal spores and more than 50 % of airborne bacteria larger than 1.1 mu m. However the biological performance of the sampler determined using a culturable bacterial counting method was significantly affected by environmental conditions, characteristics of sampler type, and consequently caused an underestimation in quantification. Therefore, in Chapter 2 and Chapter 3, Quantitative Real-Time Polymeratse Chain Reaction (QPCR) was applied to count the total bioaerol number in air samples. The results showed that successful bioaerosol quantification using QPCR requires not only to understand the characteristics of bioaerosol to be investigated and its sampling methodology, but also to develop study-specific standard curves. To increase the reliability of the method, the study-specific standrad curves associated with factors such as bacterial species, cell suspenssion preparation methods, QPCR methods should be developed and used for quantifications. To this end, the developed QPCR assay was applied to test the performance of a novel bioaerosol sampler (EPSS). The test results indicated a successful application of QPCR method to test performance of bioaerosol samplers. By coupling with an effective bioaerosol sampling device, this QPCR assay could increase the reliability of bioaerosol sampling systems and allow timely and effective quantification of aerosol samples.
Overall, the findings in this dissertation provide the general guidelines to develop an effective bioaerosol monitoring system by setting-up the study-specific protocol of QPCR assay capable of determining total cell numbers in air samples. The improved bioaerosol sampling system enabling rapid quantification of bioaerosols with high sensitivity may be applied as a basis for developing bioaerosol detection systems capable of detecting even small bioaerosol concentrations thus providing useful information needed to understand the bioaerosol exposure dose and response relationship.
PhysicalDescription
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electronic resource
Extent
xii, 159 p. : ill.
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Note (type = degree)
Ph.D.
Note (type = bibliography)
Includes bibliographical references
Note (type = statement of responsibility)
by Hey Reoun An
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An
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Hey Reoun
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Hey Reoun An
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Mainelis
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chair
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Gediminas Mainelis
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Turpin
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Barbara
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internal member
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Barbara Turpin
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Fennell
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Donna
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Donna Fennell
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White
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Lori
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outside member
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Advisory Committee
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Lori White
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Rutgers University
Role
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degree grantor
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Graduate School - New Brunswick
Role
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school
OriginInfo
DateCreated (point = ); (qualifier = exact)
2009
DateOther (qualifier = exact); (type = degree)
2009-05
Place
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xx
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TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
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TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
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NjNbRU
Identifier (type = doi)
doi:10.7282/T3251JDT
Genre (authority = ExL-Esploro)
ETD doctoral
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The author owns the copyright to this work.
Copyright
Status
Copyright protected
Availability
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Open
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Non-exclusive ETD license
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Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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