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Explore the function of KEL-8 in oxidative stress response and search for KEL-8 interacting proteins

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Text
TitleInfo (ID = T-1)
Title
Explore the function of KEL-8 in oxidative stress response and search for KEL-8 interacting proteins
SubTitle
PartName
PartNumber
NonSort
Identifier
ETD_1637
Identifier (type = hdl)
http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000051183
Language (objectPart = )
LanguageTerm (authority = ISO639-2); (type = )
eng
Genre (authority = marcgt)
theses
Subject (ID = SBJ-1); (authority = RUETD)
Topic
Cell and Developmental Biology
Subject (ID = SBJ-2); (authority = ETD-LCSH)
Topic
Oxidative stress
Abstract
Previous work in our lab identified KEL-8, a BTB-Kelch superfamily protein, as a substrate receptor for a Cul3 ubiquitin ligase that plays a role in the turnover of GLR-1 AMPA-type glutamate receptors in neurons. C. elegans kel-8 is similar in sequence to Keap1, which encodes a BTB-Kelch protein that negatively regulates the oxidative stress response pathway, leading us to hypothesize that kel-8 is also involved in oxidative stress response, repressing the expression of oxidative stress response genes. We have two major questions: what is the role of KEL-8 in oxidative stress and what are the targets of KEL-8. In order to answer the first question, we used the oxidative stress reporter transgene Pgcs-1::gfp. The gcs-1 gene encodes a phase II detoxification enzyme, γ--glutamine cysteine synthase, and is expressed in the intestine in response to oxidative stress reagents. I tested the expression level of Pgcs-1::gfp in kel-8(od38) nonsense mutants and found that the gcs-1 expression level in kel-8 is constitutively high compared to wild-type animals. Our preliminary results indicate that KEL-8 functions similarly to Keap1 to repress the oxidative stress response. However, the repression phenotype is inconsistent. In addition, we did a yeast two-hybrid screen to search for targets or other interacting proteins of KEL-8, and we found 8 candidates. They are ZC434.8, ric-4, F32D8.12, pccb-1, ssq-4, dim-1, aly-3, and smo-1. Further analyses need to be done to determine whether these are biological meaningful interactions.
PhysicalDescription
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electronic resource
Extent
v, 86 p. : ill.
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application/pdf
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text/xml
Note (type = degree)
M.S.
Note (type = bibliography)
Includes bibliographical references (p. 77-86)
Note (type = statement of responsibility)
by Daiying Chen
Name (ID = NAME-1); (type = personal)
NamePart (type = family)
Chen
NamePart (type = given)
Daiying
NamePart (type = date)
1984
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author
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Daiying Chen
Name (ID = NAME-2); (type = personal)
NamePart (type = family)
Rongo
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Christopher
Role
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chair
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Advisory Committee
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Christopher Rongo
Name (ID = NAME-3); (type = personal)
NamePart (type = family)
Driscoll
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Monica
Role
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co-chair
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Advisory Committee
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Monica Driscoll
Name (ID = NAME-4); (type = personal)
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Grant
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Barth
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internal member
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Advisory Committee
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Barth Grant
Name (ID = NAME-5); (type = personal)
NamePart (type = family)
Barr
NamePart (type = given)
Maureen
Role
RoleTerm (authority = RULIB); (type = )
internal member
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Advisory Committee
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Maureen Barr
Name (ID = NAME-1); (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB); (type = )
degree grantor
Name (ID = NAME-2); (type = corporate)
NamePart
Graduate School - New Brunswick
Role
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school
OriginInfo
DateCreated (point = ); (qualifier = exact)
2009
DateOther (qualifier = exact); (type = degree)
2009-05
Place
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xx
Location
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NjNbRU
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TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
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TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Identifier (type = doi)
doi:10.7282/T36W9B9H
Genre (authority = ExL-Esploro)
ETD graduate
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Open
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Non-exclusive ETD license
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I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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