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Analysis of sperm molecules needed for ferilization in C. elegans
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Text
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Title
Analysis of sperm molecules needed for ferilization in C. elegans
Identifier
ETD_1534
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http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000051349
Language
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eng
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theses
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Topic
Cell and Developmental Biology
Subject
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(authority = ETD-LCSH)
Topic
Caenorhabditis elegans--Genetics
Subject
(ID = SBJ-1);
(authority = ETD-LCSH)
Topic
Fertilization (Biology)
Abstract
Fertilization is the biological process through which life is propagated and genetic variation is generated. Its importance to sexually reproducing species is unrivaled, but we are far from obtaining a complete understanding of how this process occurs. This thesis is a study of sperm molecules involved in Caenorhabditis elegans fertilization with the ultimate goal of elucidating and understanding the function of fertilization molecules across species.
The first chapter provides an introduction to the field of fertilization, a brief overview of what is currently known, and it concludes with the use and advantages of using C. elegans as a model system to study fertilization.
The second chapter describes a method that utilizes single nucleotide polymorphisms (SNPs) to streamline the initial genetic mapping of newly isolated mutants. An example of this method for linkage mapping is provided, and I compare and contrast this method to traditional and other SNP approaches.
The third chapter describes the phenotypic characterization and initial mapping of the as28 mutation resulting in a temperature sensitive sterility in C. elegans. The mutation causes a sperm activation defect causing abnormal spermatid morphology. Mapping of this mutant strain revealed that the observed phenotype is the result of two mutations, which has complicated characterization of this mutant phenotype.
The fourth chapter describes the cloning of spe-13. Mutations in spe-13 result in worms that produce morphologically and physiologically normal spermatids that mature into spermatozoa that are unable to fertilize oocytes. The identification of spe-13 has proved to be difficult with traditional mapping techniques due to its chromosomal position. Using whole genome sequencing, we identified spe-13 candidate genes in an effort to clone this elusive gene.
In the final chapter, I summarize and discuss future directions for each project that has been described in this thesis work.
The first chapter provides an introduction to the field of fertilization, a brief overview of what is currently known, and it concludes with the use and advantages of using C. elegans as a model system to study fertilization.
The second chapter describes a method that utilizes single nucleotide polymorphisms (SNPs) to streamline the initial genetic mapping of newly isolated mutants. An example of this method for linkage mapping is provided, and I compare and contrast this method to traditional and other SNP approaches.
The third chapter describes the phenotypic characterization and initial mapping of the as28 mutation resulting in a temperature sensitive sterility in C. elegans. The mutation causes a sperm activation defect causing abnormal spermatid morphology. Mapping of this mutant strain revealed that the observed phenotype is the result of two mutations, which has complicated characterization of this mutant phenotype.
The fourth chapter describes the cloning of spe-13. Mutations in spe-13 result in worms that produce morphologically and physiologically normal spermatids that mature into spermatozoa that are unable to fertilize oocytes. The identification of spe-13 has proved to be difficult with traditional mapping techniques due to its chromosomal position. Using whole genome sequencing, we identified spe-13 candidate genes in an effort to clone this elusive gene.
In the final chapter, I summarize and discuss future directions for each project that has been described in this thesis work.
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electronic resource
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vii, 65 p. : ill.
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M.S.
Note
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Includes bibliographical references (p. 58-65)
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by Julie S. Hang
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Hang
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Julie S.
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Julie S. Hang
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Mckim
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Kim
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Kim Mckim
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Singson
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Andrew
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Andrew Singson
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Driscoll
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Monica
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Monica Driscoll
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Barth
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Rutgers University
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Graduate School - New Brunswick
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2009
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2009-05
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xx
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Rutgers University Electronic Theses and Dissertations
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ETD
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Graduate School - New Brunswick Electronic Theses and Dissertations
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rucore19991600001
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Identifier
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doi:10.7282/T3S182QV
Genre
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ETD graduate
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The author owns the copyright to this work.
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Open
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Non-exclusive ETD license
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Author Agreement License
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I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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