Staff View
Variation in thyroid hormone concentration in brain microdialysates from freely-moving adult rats

Descriptive

TypeOfResource
Text
TitleInfo (ID = T-1)
Title
Variation in thyroid hormone concentration in brain microdialysates from freely-moving adult rats
Identifier
ETD_1858
Identifier (type = hdl)
http://hdl.rutgers.edu/1782.2/rucore10005600001.ETD.000051334
Language
LanguageTerm (authority = ISO639-2); (type = code)
eng
Genre (authority = marcgt)
theses
Subject (ID = SBJ-1); (authority = RUETD)
Topic
Biology
Subject (ID = SBJ-1); (authority = ETD-LCSH)
Topic
Thyroid hormones
Abstract
Thyroid hormone has genomic and non-genomic actions in the developing and mature human. The focus of this research was to find if there was a measurable amount of thyroid hormone in the extracellular fluid of the adult rat brain and how it varied over time. To study the levels of thyroid hormone concentration in the adult brain, stereotaxic surgery was performed to implant a microdialysis cannula and electrodes for the study of electroencephalogram (EEG), electromyogram (EMG). The stylet blocking the microdialysis cannula was removed and the probe was inserted at the beginning of the study for sample collection. EEG and EMG waves were recorded concurrently. Artificial cerebrospinal fluid (aCSF) was run through the inlet tube of the probe and sample was obtained from the outlet using a refrigerated fraction collector. Collected samples were analyzed by high pressure liquid chromatography (HPLC), liquid chromatography mass spectrometry (LC/MS) and radioimmunoassay (RIA) for presence of the thyroid hormone. Samples run through the C18 column on the HPLC gave a peak for thyroid hormone at a retention time of 4.1minutes. Similar results were generated when the microdialysate samples were analyzed using LC/MS. The LC of the microdialysate gave the peak at the retention time of 4.1 minutes. This peak was further studied for confirmation by MS ionization. MS gave a fragmentation pattern which corresponded to the breakdown products of the thyroid hormone. The RIA kit with thyroid hormone standards prepared in aCSF was used for the detection of the thyroid hormone concentration. The level of thyroid hormone in samples varies over time. The variation in thyroid hormone level over time was compared to the sleep data obtained concurrently. The variation seen in the thyroid hormone concentration over time was detected by HPLC, LC/MS and RIA. This variation suggests some possibility of a mechanism of release of thyroid hormone in adult brain. This finding would be consistent with the potential signaling role of TH suggested by earlier experiments performed in our lab.
PhysicalDescription
Form (authority = gmd)
electronic resource
Extent
vii, 56 p. : ill.
InternetMediaType
application/pdf
InternetMediaType
text/xml
Note (type = degree)
M.S.
Note (type = bibliography)
Includes bibliographical references (p. 51-56)
Note (type = statement of responsibility)
by Namrata Choudhari
Name (ID = NAME-1); (type = personal)
NamePart (type = family)
Choudhari
NamePart (type = given)
Namrata
NamePart (type = date)
1979
Role
RoleTerm (authority = RULIB); (type = )
author
DisplayForm
Namrata Choudhari
Name (ID = NAME-2); (type = personal)
NamePart (type = family)
Martin
NamePart (type = given)
Joseph
Role
RoleTerm (authority = RULIB); (type = )
chair
Affiliation
Advisory Committee
DisplayForm
Joseph V. Martin
Name (ID = NAME-3); (type = personal)
NamePart (type = family)
McIlroy
NamePart (type = given)
Patrick
Role
RoleTerm (authority = RULIB); (type = )
internal member
Affiliation
Advisory Committee
DisplayForm
Patrick J. McIlroy
Name (ID = NAME-4); (type = personal)
NamePart (type = family)
Saidel
NamePart (type = given)
William
Role
RoleTerm (authority = RULIB); (type = )
outside member
Affiliation
Advisory Committee
DisplayForm
William M. Saidel
Name (ID = NAME-1); (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB); (type = )
degree grantor
Name (ID = NAME-2); (type = corporate)
NamePart
Camden Graduate School
Role
RoleTerm (authority = RULIB); (type = )
school
OriginInfo
DateCreated (point = ); (qualifier = exact)
2009
DateOther (qualifier = exact); (type = degree)
2009-05
Place
PlaceTerm (type = code)
xx
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
RelatedItem (type = host)
TitleInfo
Title
Camden Graduate School Electronic Theses and Dissertations
Identifier (type = local)
rucore10005600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T3W37WJS
Genre (authority = ExL-Esploro)
ETD graduate
Back to the top

Rights

RightsDeclaration (AUTHORITY = GS); (ID = rulibRdec0006)
The author owns the copyright to this work.
Copyright
Status
Copyright protected
Availability
Status
Open
RightsEvent (AUTHORITY = rulib); (ID = 1)
Type
Permission or license
Detail
Non-exclusive ETD license
AssociatedObject (AUTHORITY = rulib); (ID = 1)
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Back to the top

Technical

ContentModel
ETD
MimeType (TYPE = file)
application/pdf
MimeType (TYPE = container)
application/x-tar
FileSize (UNIT = bytes)
522240
Checksum (METHOD = SHA1)
42b4dc5be9b85bf960ceb0047711f20b5b0b875f
Back to the top
Version 8.5.5
Rutgers University Libraries - Copyright ©2024