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Nitrogen metabolism and transport in the Arbuscular mycorrhizal interaction

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TypeOfResource
Text
TitleInfo (ID = T-1)
Title
Nitrogen metabolism and transport in the Arbuscular mycorrhizal interaction
Identifier
ETD_2006
Identifier (type = hdl)
http://hdl.rutgers.edu/1782.2/rucore10005600001.ETD.000051639
Language
LanguageTerm (authority = ISO639-2); (type = code)
eng
Genre (authority = marcgt)
theses
Subject (ID = SBJ-1); (authority = RUETD)
Topic
Biology
Subject (ID = SBJ-1); (authority = ETD-LCSH)
Topic
Mycorrhizal fungi
Subject (ID = SBJ-1); (authority = ETD-LCSH)
Topic
Nitrogen--Metabolism
Abstract
The Arbuscular Mycorrhiza is arguably the world's most important interaction. The roots of over 70 % of all known land plant species form this mutualistic interaction with fungi of the phylum Glomeromycota. Arbuscular mycorrhizal (AM) fungi can act as biofertilizers, bioprotectors and bioregulators of plants and have gained increasing attention for their potential role in sustainable agriculture and in the restoration and bioremediation of contaminated and disturbed sites. In this interaction plants take up mineral nutrients from the soil through their associated AM fungi and transfer in exchange for their beneificial effect on nutrient uptake photosynthetically fixed carbon to the fungus. Nitrogen (N) is known to be transferred from the fungus to the plant in the AM interaction, yet its metabolism, storage and transport in the symbiosis are poorly understood. Here, we report new findings about the N metabolism and transport in the AM symbiosis by analyzing fungal gene expression with quantitative polymerase chain reaction (Q-PCR).
In vitro mycorrhizas of Glomus intraradices and Ri T-DNA-transformed carrot roots were grown in two-compartment Petri dishes. Different experiments were carried out to measure the effect of different carbon (C) or nitrogen (N) sources on fungal gene expression. The RNA was extracted from the ERM and processed for Q-PCR using gene specific primers. Inorganic nitrogen is taken up by the fungus with its extraradical mycelium (ERM), is incorporated into amino acids, and translocated from the ERM to the intraradical mycelium (IRM) as arginine, where it is broken down to an inorganic form via the catabolic arm of the urea cycle and transferred to the plant without C (Govindarajulu et al., 2005; Jin et al., 2005). Consistent with the proposed mechanism, the genes involved primarily in nitrogen assimilation were highly expressed in the ERM whereas the mRNA transcripts levels of genes associated with the breakdown of arginine were low in the ERM. The expression levels of these genes are regulated by the C availability for the mycorrhizal fungus and by an exogenous supply of N to the ERM.
PhysicalDescription
Form (authority = gmd)
electronic resource
Extent
vi, 38 p. : ill.
InternetMediaType
application/pdf
InternetMediaType
text/xml
Note (type = degree)
M.S.
Note (type = bibliography)
Includes bibliographical references (p. 34-38)
Note (type = statement of responsibility)
by Sulbha Choudhari
Name (ID = NAME-1); (type = personal)
NamePart (type = family)
Choudhari
NamePart (type = given)
Sulbha
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author
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Sulbha Choudhari
Name (ID = NAME-2); (type = personal)
NamePart (type = family)
Bucking
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Heike
Role
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chair
Affiliation
Advisory Committee
DisplayForm
Heike Bucking
Name (ID = NAME-3); (type = personal)
NamePart (type = family)
Dighton
NamePart (type = given)
John
Role
RoleTerm (authority = RULIB); (type = )
internal member
Affiliation
Advisory Committee
DisplayForm
John Dighton
Name (ID = NAME-4); (type = personal)
NamePart (type = family)
Gray
NamePart (type = given)
Dennis
Role
RoleTerm (authority = RULIB); (type = )
internal member
Affiliation
Advisory Committee
DisplayForm
Dennis Gray
Name (ID = NAME-1); (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB); (type = )
degree grantor
Name (ID = NAME-2); (type = corporate)
NamePart
Camden Graduate School
Role
RoleTerm (authority = RULIB); (type = )
school
OriginInfo
DateCreated (point = ); (qualifier = exact)
2009
DateOther (qualifier = exact); (type = degree)
2009-10
Place
PlaceTerm (type = code)
xx
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
RelatedItem (type = host)
TitleInfo
Title
Camden Graduate School Electronic Theses and Dissertations
Identifier (type = local)
rucore10005600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T3NK3F8Z
Genre (authority = ExL-Esploro)
ETD graduate
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The author owns the copyright to this work.
Copyright
Status
Copyright protected
Availability
Status
Open
RightsEvent (AUTHORITY = rulib); (ID = 1)
Type
Permission or license
Detail
Non-exclusive ETD license
AssociatedObject (AUTHORITY = rulib); (ID = 1)
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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Technical

ContentModel
ETD
MimeType (TYPE = file)
application/pdf
MimeType (TYPE = container)
application/x-tar
FileSize (UNIT = bytes)
2222080
Checksum (METHOD = SHA1)
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