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Generation and characterization of random and site-directed mutants of Shiga-like toxin 1A by Escherichia Coli O157:H7 in Saccharomyces Cerevisiae
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TypeOfResource
Text
TitleInfo (ID = T-1)
Title
Generation and characterization of random and site-directed mutants of Shiga-like toxin 1A by Escherichia Coli O157:H7 in Saccharomyces Cerevisiae
Identifier
ETD_1748
Identifier (type = hdl)
http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000051406
Language
LanguageTerm (authority = ISO639-2); (type = code)
eng
Genre (authority = marcgt)
theses
Subject (ID = SBJ-1); (authority = RUETD)
Topic
Food Science
Subject (ID = SBJ-1); (authority = ETD-LCSH)
Topic
Escherichia coli
Subject (ID = SBJ-1); (authority = ETD-LCSH)
Topic
Saccharomyces cerevisiae
Subject (ID = SBJ-1); (authority = ETD-LCSH)
Topic
Mutation (Biology)
Abstract
Food-borne illnesses are mainly associated with Shiga-like toxins (Stx1 and Stx2) produced by various serotypes of enterohemorrhagic Escherichia coli (EHEC). These serotypes are collectively known as STEC (Stx-producing E. coli). One of these serotypes E. coli O157:H7 has been the major cause of food-borne illnesses recently in US, Canada and Japan. The clinical manifestations of EHEC infections range from watery diarrhea, severe bloody diarrhea, abdominal cramps and hemorrhagic colitis (HC), to the most severe outcome, life-threatening hemolytic uremic syndrome (HUS) resulting in kidney failure.
This study involved generation and characterization of random and site-directed mutants of Shiga-like toxin 1 in Saccharomyces cerevisiae. The mutants were characterized for protein expression, ribosome depurination and loss of cytotoxicity. The results from this study are crucial to understand the mechanism by which Shiga-like toxins exhibit their cytotoxicity to the cells. The results from this study can aid in development of treaments against the diseases caused by Shiga-like toxin 1.
PhysicalDescription
Form (authority = gmd)
electronic resource
Extent
v, 35 p. : ill.
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application/pdf
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text/xml
Note (type = degree)
M.S.
Note (type = bibliography)
Includes bibliographical references (p. 33-35)
Note (type = statement of responsibility)
by Varsha Shete
Name (ID = NAME-1); (type = personal)
NamePart (type = family)
Shete
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Varsha
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1982
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author
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Varsha Shete
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Tumer
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Nilgun
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chair
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Advisory Committee
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Nilgun Tumer
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Di
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Rong
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co-chair
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Advisory Committee
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Rong Di
Name (ID = NAME-4); (type = personal)
NamePart (type = family)
Matthews
NamePart (type = given)
Karl
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internal member
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Advisory Committee
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Karl Matthews
Name (ID = NAME-1); (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB); (type = )
degree grantor
Name (ID = NAME-2); (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB); (type = )
school
OriginInfo
DateCreated (point = ); (qualifier = exact)
2009
DateOther (qualifier = exact); (type = degree)
2009-05
Place
PlaceTerm (type = code)
xx
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
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TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
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NjNbRU
Identifier (type = doi)
doi:10.7282/T300029Z
Genre (authority = ExL-Esploro)
ETD graduate
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Rights

RightsDeclaration (AUTHORITY = GS); (ID = rulibRdec0006)
The author owns the copyright to this work.
Copyright
Status
Copyright protected
Availability
Status
Open
RightsEvent (AUTHORITY = rulib); (ID = 1)
Type
Permission or license
Detail
Non-exclusive ETD license
AssociatedObject (AUTHORITY = rulib); (ID = 1)
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License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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Technical

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ETD
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application/pdf
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application/x-tar
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1669120
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30a5762ef9525550159cc574e68ce1d46ab99f85
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