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Maximizing the health benefits of lycopene isomers
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Text
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Title
Maximizing the health benefits of lycopene isomers
Identifier
ETD_1554
Identifier
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http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000051418
Language
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eng
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theses
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Topic
Food Science
Subject
(ID = SBJ-1);
(authority = ETD-LCSH)
Topic
Lycopene
Subject
(ID = SBJ-1);
(authority = ETD-LCSH)
Topic
Tomatoes--Health aspects
Subject
(ID = SBJ-1);
(authority = ETD-LCSH)
Topic
Diet therapy
Abstract
Lycopene is an acyclic C40 non polar carotenoid found in tomatoes. In nature, the predominant form is the all-trans isomeric form which represents 80-97% of lycopene in tomato and tomato related products. However on consumption of tomato products, >50% of the lycopene is found in the cis isomeric form in the body, thereby implying that the cis isomeric form is the more bioavailable /bioactive form in the human body.
The major objective of our study was to validate the bioactivity of lycopene isomers. Three approaches were used to achieve this goal. 1). An ab initio computational model to study the structure of isomers. 2). Develop a method to isolate isomers. 3). Study antioxidant activity and cellular proliferation activity of isomers in in- vitro condition.
Computational modeling studies showed that lycopene isomers differ in their electronic distribution on the molecules. Cellular Antioxidant Activity (CAA) method showed that cis isomers have higher CAA compared to all-trans isomers.
The cellular proliferation assay known as the MTT assay indicated larger decrease in proliferation of cis isomer treated prostate cancer cells. The anti-inflammatory assay (measures the amount of nitric oxide (NO) produced by mouse macrophages (RAW 264.7)) showed marginal difference in activity between control and isomer treated cells.
Non-thermal processing techniques like High Hydrostatic Pressure (HHP) processing and Pulse Electric Field (PEF) were used to increase the yield of beneficial cis lycopene isomer in the processed product. The results showed an qualitative increase in cis isomer after non-thermal processing.
This study helps us get deeper insights on the activity of cis and all-trans lycopene isomers at a basic molecular level, which correlates to the activity at a chemical and biological level. Non-thermal processing methods like High Hydrostatic Pressure processing and Pulse Electric Field processing methods can cause lycopene isomerization. These can be used as alternative means of processing to get tomato products that can yield health benefits to the consumers.
The major objective of our study was to validate the bioactivity of lycopene isomers. Three approaches were used to achieve this goal. 1). An ab initio computational model to study the structure of isomers. 2). Develop a method to isolate isomers. 3). Study antioxidant activity and cellular proliferation activity of isomers in in- vitro condition.
Computational modeling studies showed that lycopene isomers differ in their electronic distribution on the molecules. Cellular Antioxidant Activity (CAA) method showed that cis isomers have higher CAA compared to all-trans isomers.
The cellular proliferation assay known as the MTT assay indicated larger decrease in proliferation of cis isomer treated prostate cancer cells. The anti-inflammatory assay (measures the amount of nitric oxide (NO) produced by mouse macrophages (RAW 264.7)) showed marginal difference in activity between control and isomer treated cells.
Non-thermal processing techniques like High Hydrostatic Pressure (HHP) processing and Pulse Electric Field (PEF) were used to increase the yield of beneficial cis lycopene isomer in the processed product. The results showed an qualitative increase in cis isomer after non-thermal processing.
This study helps us get deeper insights on the activity of cis and all-trans lycopene isomers at a basic molecular level, which correlates to the activity at a chemical and biological level. Non-thermal processing methods like High Hydrostatic Pressure processing and Pulse Electric Field processing methods can cause lycopene isomerization. These can be used as alternative means of processing to get tomato products that can yield health benefits to the consumers.
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electronic resource
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xv, 142 p. : ill.
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Ph.D.
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Includes bibliographical references
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by Supriya Varma
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Varma
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Supriya
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1978
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Supriya Varma
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Lee
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Dr.Tung-Ching
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Advisory Committee
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Dr.Tung-Ching Lee
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Daun
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Dr Henryk
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Dr Henryk Daun
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Huang
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Dr Mou-Tou
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Dr Mou-Tou Huang
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Karwe
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Dr. Mukund
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Dr. Mukund Karwe
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Rutgers University
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Graduate School - New Brunswick
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2009
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2009-05
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xx
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Rutgers University Electronic Theses and Dissertations
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ETD
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Graduate School - New Brunswick Electronic Theses and Dissertations
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doi:10.7282/T3QN670B
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ETD doctoral
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Open
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Non-exclusive ETD license
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Author Agreement License
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I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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