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Microbial degradation of the fuel oxygenate methyl tert-bytyl ether (MTBE)
Descriptive
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Text
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Title
Microbial degradation of the fuel oxygenate methyl tert-bytyl ether (MTBE)
SubTitle
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ETD_2036
Identifier
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http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000051982
Language
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eng
Genre
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theses
Subject
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Topic
Microbiology and Molecular Genetics
Subject
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(authority = ETD-LCSH)
Topic
Butyl methyl ether--Biodegradation
Subject
(ID = SBJ-3);
(authority = ETD-LCSH)
Topic
In situ bioremediation
Abstract
Groundwater contamination with the synthetic fuel oxygenate, methyl tert-butyl ether (MTBE), is an extensive problem. Microbial mediated biodegradation holds promise as a tool for remediation of contaminated water supplies. However, MTBE biotransformation processes are slow and MTBE degrading organisms are difficult to isolate, creating challenges relating to site assessment, enhancement of natural attenuation and monitoring bioremediation in situ. In this study we analyzed MTBE degrading cultures using a variety of isolation independent techniques. A majority of the experiments used previously established anaerobic enrichment cultures that had been maintained on MTBE for several years. We demonstrated that low concentrations of some aryl O-methyl ether compounds enhanced the rate of MTBE degradation. Propyl iodide caused a light-reversible inhibition of MTBE depletion, suggesting that the anaerobic MTBE O-demethylation reaction was corrinoid dependent. Terminal-restriction fragment length polymorphism (T-RFLP) and sequence analysis of 16S rRNA genes from one anaerobic MTBE degrading enrichment culture showed a phylogenetically diverse population with no exact matches to previously isolated or described species. Stable isotope probing experiments verified that microorganisms from anaerobic MTBE degrading enrichment culture used 13C from 13C-MTBE for growth and cell division and that a particular subpopulation assimilated this carbon prior to the rest of the population. We also analyzed carbon and hydrogen stable isotope fractionation occurring during MTBE degradation. In anaerobic cultures, substantial fractionation of hydrogen was found only in cultures supplied with syringic acid during MTBE degradation, providing the first experimental suggestion of multiple anaerobic MTBE O-demethylation mechanisms. During aerobic MTBE degradation by the psychrophilic bacterium, Variovorax paradoxus, carbon and hydrogen fractionation were not influenced by incubation temperature during degradation. This work represents a significant contribution to the current body of knowledge about MTBE degradation and the data presented will be useful in many aspects of studying, enhancing and monitoring MTBE degradation under a variety of conditions.
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electronic resource
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x, 132 p. : ill.
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application/pdf
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Note
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Ph.D.
Note
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Includes bibliographical references (p. 112-131)
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by Laura K.G. Youngster
Name
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Youngster
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Laura K. G.
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1981-
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author
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Laura K. G. Youngster
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Haggblom
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Max
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chair
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Advisory Committee
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Max M Haggblom
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Kerkhof
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Lee
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internal member
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Advisory Committee
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Lee J Kerkhof
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Barkay
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Tamar
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internal member
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Advisory Committee
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Tamar Barkay
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Fennell
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Donna
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outside member
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Advisory Committee
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Donna E Fennell
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Rutgers University
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degree grantor
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Graduate School - New Brunswick
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school
OriginInfo
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2009
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2009-10
Place
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xx
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Title
Rutgers University Electronic Theses and Dissertations
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ETD
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Title
Graduate School - New Brunswick Electronic Theses and Dissertations
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rucore19991600001
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NjNbRU
Identifier
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doi:10.7282/T3ZS2WQN
Genre
(authority = ExL-Esploro)
ETD doctoral
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Rights
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The author owns the copyright to this work.
Copyright
Status
Copyright protected
Notice
Note
Availability
Status
Open
Reason
Permission or license
Note
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Name
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Youngster
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Laura
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Laura Youngster
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Rutgers University. Graduate School - New Brunswick
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Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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Technical
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ETD
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application/pdf
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application/x-tar
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1720320
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200b0b847fe1529d252a6047f804500230684801
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