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Continuous microdialysis of blood proteins during cardiopulmonary bypass
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Continuous microdialysis of blood proteins during cardiopulmonary bypass
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ETD_2082
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http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000051821
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eng
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theses
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Biomedical Engineering
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Topic
Cardiopulmonary bypass
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Topic
Blood proteins
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Topic
Complement (Immunology)
Abstract
Cardiopulmonary bypass is a procedure that temporarily substitutes a patients’ heart and lung functions with an extracorporeal heart-lung machine. This allows surgeons to operate on motionless heart and lungs, while still providing the body with proper blood circulation. However, the heart-lung machine has been shown to activate the body’s systemic inflammatory response, resulting in short- and long- term organ dysfunction, and even death. The severity of this inflammatory response is strongly correlated to the production levels of specific cytokines and complements found in the bloodstream. Current detection methods require taking discrete blood samples during surgery and waiting at least several hours, but typically one to two weeks when including laboratory queue times in hospitals, for results. I propose a microdialysis device that continuously samples the patient’s blood for biomarkers during surgery. The primary function of this device is to prepare a purified solution, with complement concentrations that closely matches that of the patient’s bloodstream, to be used in a continuous microimmunoassay device.
The microdialysis device was fabricated using photolithography and softlithography techniques to create microfluidic channels and bonded to commercially available semi-permeable membranes with biocompatible epoxy. The device was designed based on computational simulations and fabrication constraints for optimal performance. It was tested for its analyte-recovery capabilities for complements C3a, C4a, and C5a, in human blood continually circulating through a mock heart-lung machine.
Two slightly different designs were tested, one using a membrane pore-diameter of 0.1 µm and the other using 0.4 µm. Both devices operated at a perfusion flowrate of 4.1 µL/min, which is considered to be relatively fast for microdialysis probes. For the 0.1 µm pore membrane device, the relative recoveries were 79%, 75%, and 70% for C3a, C4a, and C5a, respectively. For the 0.4 µm pore membrane device, the relative recoveries were 112%, 135%, and 101% for C3a, C4a, and C5a, respectively. These findings show promising results for the proposed microdialysis device, but further investigation is needed to improve statistical significance.
The microdialysis device was fabricated using photolithography and softlithography techniques to create microfluidic channels and bonded to commercially available semi-permeable membranes with biocompatible epoxy. The device was designed based on computational simulations and fabrication constraints for optimal performance. It was tested for its analyte-recovery capabilities for complements C3a, C4a, and C5a, in human blood continually circulating through a mock heart-lung machine.
Two slightly different designs were tested, one using a membrane pore-diameter of 0.1 µm and the other using 0.4 µm. Both devices operated at a perfusion flowrate of 4.1 µL/min, which is considered to be relatively fast for microdialysis probes. For the 0.1 µm pore membrane device, the relative recoveries were 79%, 75%, and 70% for C3a, C4a, and C5a, respectively. For the 0.4 µm pore membrane device, the relative recoveries were 112%, 135%, and 101% for C3a, C4a, and C5a, respectively. These findings show promising results for the proposed microdialysis device, but further investigation is needed to improve statistical significance.
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electronic resource
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xvii, 105 p. : ill.
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M.S.
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Includes bibliographical references (p. 89-94)
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by Alexander Fok
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Fok
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Alexander
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1983-
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Alexander Fok
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Zahn
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Jeffrey
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Jeffrey Zahn
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Cai
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Li
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Li Cai
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Shreiber
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David
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David Shreiber
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Rutgers University
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Graduate School - New Brunswick
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2009
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2009-10
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xx
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Rutgers University Electronic Theses and Dissertations
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Graduate School - New Brunswick Electronic Theses and Dissertations
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doi:10.7282/T3M32VXX
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ETD graduate
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The author owns the copyright to this work
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Alexander
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Alexander Fok
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Rutgers University. Graduate School - New Brunswick
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I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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