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Loss of the cbd-1 gene causes intracellular trafficking defects in C. elegans

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TypeOfResource
Text
TitleInfo (ID = T-1)
Title
Loss of the cbd-1 gene causes intracellular trafficking defects in C. elegans
SubTitle
PartName
PartNumber
NonSort
Identifier (displayLabel = ); (invalid = )
ETD_2070
Identifier (type = hdl)
http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000051842
Language (objectPart = )
LanguageTerm (authority = ISO639-2); (type = code)
eng
Genre (authority = marcgt)
theses
Subject (ID = SBJ-1); (authority = RUETD)
Topic
Cell and Developmental Biology
Subject (ID = SBJ-2); (authority = ETD-LCSH)
Topic
Caenorhabditis elegans--Genetics
Subject (ID = SBJ-3); (authority = ETD-LCSH)
Topic
Cell interaction
Abstract
Several large scale RNAi screens in C. elegans have allowed us to develop a network of genes involved in early embryogenesis that can be broken down into molecular modules. Phenotypic analysis of the cbd-1 gene, chosen from the oocyte integrity module has helped elucidate its role in intracellular trafficking events important for fertilization and egg activation. RNAi depletion of cbd-1 prevented sperm receptor molecules EGG-1 and EGG-2 from accumulating at the oocyte plasma membrane, thereby interfering with fertilization. Similarly, yolk receptor RME-2 did not localize to the membrane and RNAi animals showed reduced yolk uptake in oocytes, causing them to be poorly developed. Yolk uptake via RME-2 is important for CAV-1 localization, which plays a role in Ras/MAPK dependent meiotic progression. RNAi of cbd-1 caused a mislocalization of CAV-1 and a meiotic arrest at metaphase I. The EGG-3/CHS-1 membrane complex also failed to form, thereby affecting regulation of MBK-2 which did not properly degrade its maternal protein targets.
These RNAi experiments lead us to believe that cbd-1 functions as a general regulator, controlling trafficking of proteins to the membrane. Proper localization of cbd-1 targets to the membrane is critical to their function. The targets that we have uncovered thus far are important regulators of meiotic progression, sperm entry, eggshell formation, and egg activation events.
PhysicalDescription
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electronic resource
Extent
vii, 48 p. : ill.
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application/pdf
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Note (type = degree)
M.S.
Note (type = bibliography)
Includes bibliographical references (p. 44-48)
Note (type = statement of responsibility)
by Lindsay Kelly
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Kelly
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Lindsay
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1985-
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author
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Lindsay Kelly
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Singson
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Andrew
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chair
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Advisory Committee
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Andrew Singson
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Grant
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Barth
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internal member
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Advisory Committee
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Barth Grant
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Rongo
NamePart (type = given)
Chris
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internal member
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Advisory Committee
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Chris Rongo
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NamePart
Rutgers University
Role
RoleTerm (authority = RULIB); (type = )
degree grantor
Name (ID = NAME-2); (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB); (type = )
school
OriginInfo
DateCreated (point = ); (qualifier = exact)
2009
DateOther (qualifier = exact); (type = degree)
2009-10
Place
PlaceTerm (type = code)
xx
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TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
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TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
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NjNbRU
Identifier (type = doi)
doi:10.7282/T3TT4R47
Genre (authority = ExL-Esploro)
ETD graduate
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RightsDeclaration (AUTHORITY = GS); (ID = rulibRdec0006)
The author owns the copyright to this work
Copyright
Status
Copyright protected
Notice
Note
Availability
Status
Open
Reason
Permission or license
Note
RightsHolder (ID = PRH-1); (type = personal)
Name
FamilyName
Kelly
GivenName
Lindsay
Role
Copyright holder
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Permission or license
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Place
DateTime
Detail
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Copyright holder
Name
Lindsay Kelly
Affiliation
Rutgers University. Graduate School - New Brunswick
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Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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Technical

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ETD
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application/pdf
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application/x-tar
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696320
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