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Characterization of bacterial processes in the subsurface and the atmosphere

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TypeOfResource
Text
TitleInfo (ID = T-1)
Title
Characterization of bacterial processes in the subsurface and the atmosphere
SubTitle
PartName
PartNumber
NonSort
Identifier (displayLabel = ); (invalid = )
ETD_2141
Identifier (type = hdl)
http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000051910
Language (objectPart = )
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eng
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theses
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Topic
Environmental Sciences
Subject (ID = SBJ-2); (authority = ETD-LCSH)
Topic
Groundwater--Microbiology
Subject (ID = SBJ-3); (authority = ETD-LCSH)
Topic
Groundwater--Pollution
Subject (ID = SBJ-4); (authority = ETD-LCSH)
Topic
Air--Pollution
Abstract
This dissertation describes research that seeks to expand understanding of bacterially-mediated biotransformation in subsurface groundwater and the atmosphere. In the first study a highly tetrachloroethene (PCE)-enriched culture, RU11/PCE was developed from contaminated aquifer materials. Denaturing gradient gel electrophoresis (DGGE), 16S rRNA clonal library analysis, and direct sequencing of 16S rRNA, sod and gyrB genes revealed a single bacterial species, Dehalococcoides, in the RU11/PCE culture. However, because the 16S rRNA, sod and gyrB genes of the Dehalococcoides spp. are highly conserved, the possibility that more than one strain of Dehalococcoides was present could not be conclusively eliminated. The reductive dehalogenase gene profile of the RU11/PCE culture was different than that of other previously reported Dehalococcoides pure cultures and unlike other chloroethene-respiring Dehalococcoides spp., RU11/PCE grew on PCE, TCE, cis-1,2-DCE or VC.
The second study addressed the hypothesis that the air contains an active microbial ecosystem. Rotating bioaerosol bioreactors were manufactured to keep bacteria suspended in the presence of a volatile substrate while measuring their activity. A qPCR method was developed and used along with an ATP assay, microscopy, and plate counts to enumerate airborne bacteria. Although the gas-phase reactors could retain bacteria and volatile substrates for days, results using live aerosolized Xanthobacter autotrophicus and Bacillus subtilis indicated no growth. In tests with X. autotrophicus, no culturable cells were recovered under any condition. B. subtilis aerosols from dilute substrate yielded higher culturability than aerosols from distilled water with no TSB substrate. Lack of culturability occurred despite presence of airborne bacteria over time, as measured by qPCR and ATP.
Techniques were also developed to characterize microbial communities in atmospheric samples. The bacterial components of a pooled sample of atmospheric water collected in the vicinity of Oklahoma City, OK were analyzed using DGGE and clone library analysis. From DGGE analysis, six out of eight strains detected belong to the phyla of Actinobacteria, Firmicutes, Proteobacteria and Bacteriodetes. In clone library analysis, 12 bacterial strains were identified (from 78 total) with dominant occurrence of the genera, Sphingomonas, Pedobacter, and Curtobacterium spp. The bacterial populations detected from the two methods were composed of strains of diverse origins.
PhysicalDescription
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electronic resource
Extent
xvi, 181 p. : ill.
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Note (type = degree)
Ph.D.
Note (type = bibliography)
Includes bibliographical references (p. 162-179)
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by Eun Kyeu Son
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Son
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Eun Kyeu
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1973-
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Eun Kyeu Son
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Fennell
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Donna
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Donna E Fennell
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Mainelis
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Gediminas
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Gediminas Mainelis
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Haggblom
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Max
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Max Haggblom
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White
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Lori
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Lori White
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Rutgers University
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Graduate School - New Brunswick
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OriginInfo
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2009
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2009-10
Place
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xx
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Title
Rutgers University Electronic Theses and Dissertations
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ETD
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Graduate School - New Brunswick Electronic Theses and Dissertations
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rucore19991600001
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NjNbRU
Identifier (type = doi)
doi:10.7282/T34Q7V4C
Genre (authority = ExL-Esploro)
ETD doctoral
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The author owns the copyright to this work
Copyright
Status
Copyright protected
Notice
Note
Availability
Status
Open
Reason
Permission or license
Note
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Son
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Eun Kyeu
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Eun Kyeu Son
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Rutgers University. Graduate School - New Brunswick
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Author Agreement License
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I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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