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Energy storage in Chlamydomonas reinhardtii measured with photoacoustic techniques
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Text
TitleInfo
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Title
Energy storage in Chlamydomonas reinhardtii measured with photoacoustic techniques
Identifier
ETD_2094
Identifier
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http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000051927
Language
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eng
Genre
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theses
Subject
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(authority = RUETD)
Topic
Oceanography
Subject
(ID = SBJ-2);
(authority = ETD-LCSH)
Topic
Chlamydomonas reinhardtii
Subject
(ID = SBJ-3);
(authority = ETD-LCSH)
Topic
Microorganisms
Subject
(ID = SBJ-4);
(authority = ETD-LCSH)
Topic
Optoacoustic spectroscopy
Abstract
The energy storage efficiencies of the reaction centers in the intact cells of Chlamydomonas reinhardtii wild type cell, PSI-less mutants and PSII-less mutants in microsecond time window were determined using pulsed, time-resolved photoacoustic techniques. The heat emission from the photochemical reaction can result in the positive thermal expansion photoacoustic signal, opposing the negative thylokoid volume contraction signal caused by electrostriction during the charge separation. In this present research, we observed that PSI differed strongly from PSII, both in thermal expansion and volume contraction. Similar to the bacterial reaction centre, PSI is marked with the large volume contraction but small thermal expansion, in contrast to the large thermal expansion but small volume contraction in PSII. For wild type, the volume contraction signal is dominant over the thermal expansion signal upon low pulse energy illumination at room temperature. In microsecond time scale, the energy storage efficiencies were estimated to be 36%, 80±5%, and 50±14% per trap in wild type, PSI and PSII, respectively. The different energy conversion efficiencies are probably attributed to the escape of the bound counterions from the particle surface in PSI and rapid electron transfer in PSII. In the present work, we conducted the photoacoustic experiments to study the energy storage in different photosystem with the intact cells of PSI-less and PSII-less mutants, instead of using either specific wavelength excitation light or PSII inhibitors (e. g. DCMU), which were commonly applied in the previous studies. We also proved photoacoustic techniques’ usefulness and convenience in the photosynthesis research, especially in electron transfer and some related thermodynamics studies.
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electronic resource
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iii, vii, 45 p. : ill.
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Note
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M.S.
Note
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Includes bibliographical references (p. 22-27)
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by Chengyi Yan
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Yan
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Chengyi
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1979-
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author
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Chengyi Yan
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Falkowski
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Paul
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chair
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Paul G. Falkowski
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Schofield
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Oscar
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Oscar M. E. Schofield
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Gorbunov
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Maxim
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Maxim Y. Gorbunov
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Rutgers University
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degree grantor
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Graduate School - New Brunswick
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OriginInfo
DateCreated
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2009
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2009-10
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Rutgers University Electronic Theses and Dissertations
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ETD
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Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier
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rucore19991600001
Identifier
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doi:10.7282/T3K35TSJ
Genre
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ETD graduate
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The author owns the copyright to this work
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Copyright protected
Notice
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Availability
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Open
Reason
Permission or license
Note
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Yan
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Chengyi
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Chengyi Yan
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Rutgers University. Graduate School - New Brunswick
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Author Agreement License
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I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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1658880
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