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Label-free immunosensor for toxin detection in food matrix

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TypeOfResource
Text
TitleInfo (ID = T-1)
Title
Label-free immunosensor for toxin detection in food matrix
SubTitle
PartName
PartNumber
NonSort
Identifier (displayLabel = ); (invalid = )
ETD_1235
Identifier (type = hdl)
http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000051965
Language (objectPart = )
LanguageTerm (authority = ISO639-2); (type = code)
eng
Genre (authority = marcgt)
theses
Subject (ID = SBJ-1); (authority = RUETD)
Topic
Food Science
Subject (ID = SBJ-2); (authority = ETD-LCSH)
Topic
Biosensors
Subject (ID = SBJ-3); (authority = ETD-LCSH)
Topic
Detectors
Subject (ID = SBJ-4); (authority = ETD-LCSH)
Topic
Food--Analysis
Abstract
A method for the detection of biological toxins in foods has to be sensitive, rapid, and field-applicable since toxin detection is on the front line of food safety and food protection against bioterrorism. In this thesis a sensitive, rapid, and label-free electrochemical impedance spectroscopy (EIS) immunosensor was developed and its performance in real foods was demonstrated.
To improve the sensitive of EIS immunosensor, nanoporous aluminum was applied as the substrate of EIS immunosensor. Well ordered nanoporous aluminum having ~30nm in pore diameter was obtained by anodizing food grade aluminum in 0.3M oxalic acid at 40V. EIS immunosensor for the detection of staphylococcal enterotoxin B (SEB) or ricin was developed by immobilizing anti-SEB or ricin on nanoporous aluminum using 3-aminopropyltriethoxysilane (APTES). Particularly efficient immobilization of antibody (Ab) was attained by silanization of aluminum in 2% APTES for 4hrs.
A time-resolved EIS of immunosensor was performed to investigate the effect of immunoreaction on impedimetric signal outputs. Immunoreaction between immobilized Ab and its target toxin resulted in gradual changes in the spectra of impedimetric signal outputs. The change by immunoreaction was significant especially in real part of impedance (Z') at the frequency region from 10kHz to 100kHz. The specific changes of Z' to immunoreaction also could be observed at pH 3.0 and pH 5.0. Using developed EIS immunosensor, it was possible to detect the presence of 10pg/ml of SEB in 15min without amplification of signal output such as labeling step. Moreover EIS immunosensor was applicable to real foods even to acidic foods.
PhysicalDescription
Form (authority = gmd)
electronic resource
Extent
xiii, 128 p. : ill.
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application/pdf
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Note (type = degree)
Ph.D.
Note (type = bibliography)
Includes bibliographical references
Note (type = statement of responsibility)
by Changhoon Chai
Name (ID = NAME-1); (type = personal)
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Chai
NamePart (type = given)
Chang-Hoon
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author
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Chang-Hoon Chai
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Takhistov
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Paul
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chair
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Advisory Committee
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Paul Takhistov
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NamePart (type = family)
Yam
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Kit
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internal member
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Advisory Committee
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Kit L. Yam
Name (ID = NAME-4); (type = personal)
NamePart (type = family)
Huang
NamePart (type = given)
Qingrong
Role
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internal member
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Advisory Committee
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Qingrong Huang
Name (ID = NAME-5); (type = personal)
NamePart (type = family)
Dunn
NamePart (type = given)
Stanley
Role
RoleTerm (authority = RULIB); (type = )
outside member
Affiliation
Advisory Committee
DisplayForm
Stanley Dunn
Name (ID = NAME-1); (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB); (type = )
degree grantor
Name (ID = NAME-2); (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB); (type = )
school
OriginInfo
DateCreated (point = ); (qualifier = exact)
2008
DateOther (qualifier = exact); (type = degree)
2008-10
Place
PlaceTerm (type = code)
xx
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
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NjNbRU
Identifier (type = doi)
doi:10.7282/T3CF9Q8P
Genre (authority = ExL-Esploro)
ETD doctoral
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Rights

RightsDeclaration (AUTHORITY = GS); (ID = rulibRdec0006)
The author owns the copyright to this work
Copyright
Status
Copyright protected
Notice
Note
Availability
Status
Open
Reason
Permission or license
Note
RightsHolder (ID = PRH-1); (type = personal)
Name
FamilyName
Chai
GivenName
Chang-Hoon
Role
Copyright holder
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Type
Permission or license
Label
Place
DateTime
Detail
AssociatedEntity (ID = AE-1); (AUTHORITY = rulib)
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Copyright holder
Name
Chang-Hoon Chai
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject (ID = AO-1); (AUTHORITY = rulib)
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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Technical

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ETD
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application/pdf
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application/x-tar
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2805760
Checksum (METHOD = SHA1)
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