RUcore: Rutgers University Community Repository
Polymeric nanospheres for skin penetration enhancement
Descriptive
TypeOfResource
Text
TitleInfo
(ID = T-1)
Title
Polymeric nanospheres for skin penetration enhancement
SubTitle
in vitro and in vivo assessment in skin models
PartName
PartNumber
NonSort
Identifier
(displayLabel = );
(invalid = )
ETD_2322
Identifier
(type = hdl)
http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000052097
Language
(objectPart = )
LanguageTerm
(authority = ISO639-2);
(type = code)
eng
Genre
(authority = marcgt)
theses
Subject
(ID = SBJ-1);
(authority = RUETD)
Topic
Pharmaceutical Science
Subject
(ID = SBJ-2);
(authority = ETD-LCSH)
Topic
Polymeric drug delivery systems
Subject
(ID = SBJ-3);
(authority = ETD-LCSH)
Topic
Transdermal medication
Abstract
Research and development in the field of topical and transdermal delivery has been particularly challenging due to the tough penetration barrier provided by the stratum corneum, the superficial skin layer. Nano-sized vesicles can potentially act as carriers to skin layers without causing the toxicity and irritation associated with chemical and physical skin penetration enhancers. In this study we have characterized the skin penetration potential of polymeric nanospheres made from amphiphilic ABA-triblock co-polymers that are biocompatible, biodegradable and bind efficiently to hydrophobic molecules. We evaluated the delivery of hydrophobic and poorly water soluble compounds via these nanospheres into different skin layers and found significant enhancement in their delivery to the epidermal-dermal junction and to the dermis. Thus, these nanospheres have tremendous potential for targeting diseases such as acne, psoriasis and eczema that have their origins in these layers. Formulation of the nanospheres in hydrophilic gels, alone and in combination with a chemical skin penetration enhancer significantly increased the penetration of complexed molecules in vitro in human skin and in vivo in porcine skin. We also evaluated the delivery profiles of these nanospheres in a bioengineered Human Skin Equivalent (HSE). The full thickness HSE was developed from a combination of human derived cells and extra-cellular components and cultured with a novel media cocktail to strengthen the permeability barrier. The HSE was characterized for the permeability profiles of agents with differing physiochemical properties and was found to be more permeable than human skin, but similar to the commercially available skin equivalent EpidermFT®. The HSE also served as an effective model for evaluation of phototoxicity of topically applied agents, and was able to correctly predict the phototoxic potential of compounds when evaluated against a validated in vitro cell-based method. When used for evaluation of the skin delivery potential of nanosphere formulations, the penetration enhancement ratios in the HSE were similar to those obtained in human skin and porcine skin, although the amount and depth of skin penetration of compounds was different. Thus, despite a weaker permeability barrier, the HSE can serve as a reproducible model for pre-screening of the skin delivery properties of formulations.
PhysicalDescription
Form
(authority = gmd)
electronic resource
Extent
xx, 261 p. : ill.
InternetMediaType
application/pdf
InternetMediaType
text/xml
Note
(type = degree)
Ph.D.
Note
(type = bibliography)
Includes bibliographical references (p. 237-247)
Note
(type = statement of responsibility)
by Priya B. Batheja
Name
(ID = NAME-1);
(type = personal)
NamePart
(type = family)
Batheja
NamePart
(type = given)
Priya B.
NamePart
(type = termsOfAddress)
NamePart
(type = date)
1975-
Role
RoleTerm
(authority = RULIB);
(type = )
author
Description
DisplayForm
Priya B. Batheja
Name
(ID = NAME-2);
(type = personal)
NamePart
(type = family)
Michniak-Kohn
NamePart
(type = given)
Bozena
Role
RoleTerm
(authority = RULIB);
(type = )
chair
Affiliation
Advisory Committee
DisplayForm
Bozena Michniak-Kohn
Name
(ID = NAME-3);
(type = personal)
NamePart
(type = family)
Minko
NamePart
(type = given)
Tamara
Role
RoleTerm
(authority = RULIB);
(type = )
internal member
Affiliation
Advisory Committee
DisplayForm
Tamara Minko
Name
(ID = NAME-4);
(type = personal)
NamePart
(type = family)
You
NamePart
(type = given)
Goufeng
Role
RoleTerm
(authority = RULIB);
(type = )
internal member
Affiliation
Advisory Committee
DisplayForm
Goufeng You
Name
(ID = NAME-5);
(type = personal)
NamePart
(type = family)
Dayan
NamePart
(type = given)
Nava
Role
RoleTerm
(authority = RULIB);
(type = )
outside member
Affiliation
Advisory Committee
DisplayForm
Nava Dayan
Name
(ID = NAME-1);
(type = corporate)
NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB);
(type = )
degree grantor
Name
(ID = NAME-2);
(type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm
(authority = RULIB);
(type = )
school
OriginInfo
DateCreated
(point = );
(qualifier = exact)
2010
DateOther
(qualifier = exact);
(type = degree)
2010-01
Place
PlaceTerm
(type = code)
xx
RelatedItem
(type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
(type = RULIB)
ETD
RelatedItem
(type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier
(type = local)
rucore19991600001
Location
PhysicalLocation
(authority = marcorg);
(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T3BV7GSK
Genre
(authority = ExL-Esploro)
ETD doctoral
Back to the top
Rights
RightsDeclaration
(AUTHORITY = GS);
(ID = rulibRdec0006)
The author owns the copyright to this work.
Copyright
Status
Copyright protected
Notice
Note
Availability
Status
Open
Reason
Permission or license
Note
RightsHolder
(ID = PRH-1);
(type = personal)
Name
FamilyName
Batheja
GivenName
Priya
Role
Copyright Holder
RightsEvent
(AUTHORITY = rulib);
(ID = RE-1)
Type
Permission or license
Label
Place
DateTime
2009-12-21 02:17:00
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
AssociatedEntity
(AUTHORITY = rulib);
(ID = AE-1)
Role
Copyright holder
Name
Priya Batheja
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
(AUTHORITY = rulib);
(ID = AO-1)
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
(AUTHORITY = rulib);
(ID = RE-2)
Type
Embargo
Detail
365 days
Back to the top
Technical
ContentModel
ETD
MimeType
(TYPE = file)
application/pdf
MimeType
(TYPE = container)
application/x-tar
FileSize
(UNIT = bytes)
23091200
Checksum
(METHOD = SHA1)
2b591e3bfd1d1ddf2418f6bc57514f71aeb791ed
Back to the top
Statistical Profile