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Non-intrusive characterization of properties of hydrogels
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Non-intrusive characterization of properties of hydrogels
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ETD_2343
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http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000052102
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eng
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theses
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Mechanical and Aerospace Engineering
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Colloids
Abstract
Besides biological and chemical cues, cellular behavior has been found to be affected by mechanical cues such as traction forces, surface topology and in particular mechanical properties of the substrate. In previous studies involving hydrogel substrates, mechanical characterization was performed assuming Poisson’s ratio to be equal to one-half. However, this might not be true in all cases and might alter the calculation of stiffness of hydrogels.
The present study mainly focuses on characterizing the Young's modulus (E), shear modulus (G) and Poisson's ratio (v) of soft hydrogels using a non-intrusive technique. For this purpose, an apparatus referred to as the "four magnet setup", which allows the determination of local gel elastic properties, was developed. Closed form equations involving E, G and v of the hydrogel were derived and finite element analysis was employed to validate the equations. Linear elastic properties of bis-gels and DNA gels were obtained using the apparatus and verified using rheometry and bead experiments. This is the first report in literature in which the mechanical properties consisting of E, G and v were simultaneously obtained for soft hydrogels.
A DNA gel design space involving parameters such as crosslinker concentration, side-chain concentration and lengths of DNA strands was systematically developed and the mechanical properties were evaluated using bead experiments. It was found that stiffness of DNA gels can be modulated over a wide range by modifying the various design parameters.
Addition of DNA crosslinks generates force and alters the mechanical properties, which has implications for cell and tissue culture substrate design. Two techniques have been developed to characterize the force actuating potential of DNA gels. It was found that the force generated was proportional to the elastic modulus of the gel. Also, at higher temperatures the stiffness of the gels decreased and the amount of force generated also decreased. A comparison of the force generated in both methods showed that either method can be successfully employed. The force was found to be in the range of values reported in the literature for axonal growth in spinal cord neurons.
The present study mainly focuses on characterizing the Young's modulus (E), shear modulus (G) and Poisson's ratio (v) of soft hydrogels using a non-intrusive technique. For this purpose, an apparatus referred to as the "four magnet setup", which allows the determination of local gel elastic properties, was developed. Closed form equations involving E, G and v of the hydrogel were derived and finite element analysis was employed to validate the equations. Linear elastic properties of bis-gels and DNA gels were obtained using the apparatus and verified using rheometry and bead experiments. This is the first report in literature in which the mechanical properties consisting of E, G and v were simultaneously obtained for soft hydrogels.
A DNA gel design space involving parameters such as crosslinker concentration, side-chain concentration and lengths of DNA strands was systematically developed and the mechanical properties were evaluated using bead experiments. It was found that stiffness of DNA gels can be modulated over a wide range by modifying the various design parameters.
Addition of DNA crosslinks generates force and alters the mechanical properties, which has implications for cell and tissue culture substrate design. Two techniques have been developed to characterize the force actuating potential of DNA gels. It was found that the force generated was proportional to the elastic modulus of the gel. Also, at higher temperatures the stiffness of the gels decreased and the amount of force generated also decreased. A comparison of the force generated in both methods showed that either method can be successfully employed. The force was found to be in the range of values reported in the literature for axonal growth in spinal cord neurons.
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electronic resource
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xiv, 159 p. : ill.
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Ph.D.
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Includes bibliographical references
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by Uday Chippada
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Uday
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1979-
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Noshir
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Noshir A Langrana
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Weng
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George
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George Weng
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Alberto
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David
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Rene Schloss
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Rutgers University
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2010
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2010-01
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xx
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Rutgers University Electronic Theses and Dissertations
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doi:10.7282/T30K28RW
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ETD doctoral
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The author owns the copyright to this work.
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Copyright protected
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Open
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Chippada
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Uday
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2009-12-24 12:11:49
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Uday Chippada
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Rutgers University. Graduate School - New Brunswick
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I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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