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In vitro and in vivo anti-inflammatory effects of rosmanol and carnosol isolated from rosemary

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Text
TitleInfo (ID = T-1)
Title
In vitro and in vivo anti-inflammatory effects of rosmanol and carnosol isolated from rosemary
SubTitle
PartName
PartNumber
NonSort
Identifier (displayLabel = ); (invalid = )
ETD_2296
Identifier (type = hdl)
http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000052126
Language (objectPart = )
LanguageTerm (authority = ISO639-2); (type = code)
eng
Genre (authority = marcgt)
theses
Subject (ID = SBJ-1); (authority = RUETD)
Topic
Food Science
Subject (ID = SBJ-2); (authority = ETD-LCSH)
Topic
Rosemary--Physiological effect
Subject (ID = SBJ-3); (authority = ETD-LCSH)
Topic
Anti-inflammatory agents
Abstract
Rosemary (Rosmarinus officinalis L.) leaves are widely used as food ingredient, and have been focused on their strong antioxidant, anti-inflammatory, and anti-cancer activities. Rosmanol is transformed from carnosol, one of strong antioxidant in rosemary extract, in the presence of oxygen and demonstrated that it has a potential for anti-inflammatiory activities.
Our study consists of two parts: Anti-inflammatory activity of rosmanol on LPS induced iNOS and COX-2 in RAW 264.7 cells and inhibition activity of rosmanol and carnosol on TPA inflammation in mouse ear.
In first study, rosmanol markedly inhibited LPS-stimulated iNOS and COX-2 protein and gene expression, as well as their regulated products, NO and PGE2. Treatment with rosmanol also reduced translocation of NF-κB through preventing degradation and phosphorylation of IκB. Western blot analysis showed that rosmanol significantly inhibited translocation and phosphorylation of NF-κB and STAT3, and the protein expression of C/EBPβ and C/EBPδ. Our results showed that rosmanol down-regulates iNOS and COX-2 gene expression by inhibiting the activation of NF-κB through interfering with the activation of PI3K/Akt and MAPK signal pathway.
Based on the positive results of in vitro activity of rosmanol and the similarity of chemical structure with carnosol, we designed and performed in vivo study with rosmanol and carnosol. In inhibitory activity of rosmanol and carnosol on TPA-induced persistent inflammation in mouse ear, we examined inhibitory activity of rosmanol and carnosol on production of pro-inflammatory cytokines such as Il-1β and Il-6. The expression of inflammatory COX-2 and its upstream regulators such as NF-κB and IKK-β, and its product, PGE2 were also examined. A large amount of COX-2 expression and its up-stream regulators were detected in LPS induced mouse ear. In rosmanol and carnosol treated group, expression of pro-inflammatory cytokines and PGE2 were decreased markedly. The expression of COX-2 was also reduced, which was resulted from inhibiting up-stream regulators such as NF-κB and IKK-β. In histology of rosmanol and carnosol treated groups their all inflammatory parameters are shown to be much milder condition. Also, there is significant reduced in degree of inflammation index.
Taken together, rosmanol and carnosol, abundant ingredient in rosemary might be a potent anti-inflammatory reagent.
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electronic resource
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xvi, 103 p. : ill.
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Ph.D.
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Includes bibliographical references (p. 96-102)
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by Jong Hun Lee
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Lee
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Jong Hun
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1973-
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Jong Hun Lee
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Ho
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Chi-Tang
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Chi-Tang Ho
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Daun
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Henryk
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Henryk Daun
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Huang
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Qingrong
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Qingrong Huang
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Huang
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Mou-Tuan
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Advisory Committee
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Mou-Tuan Huang
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Rutgers University
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degree grantor
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Graduate School - New Brunswick
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school
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2010
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2010-01
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xx
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Rutgers University Electronic Theses and Dissertations
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ETD
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Graduate School - New Brunswick Electronic Theses and Dissertations
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rucore19991600001
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Identifier (type = doi)
doi:10.7282/T33F4PSM
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The author owns the copyright to this work.
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Copyright protected
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Availability
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Open
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Permission or license
Note
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Lee
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Jonghun
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DateTime
2009-12-12 20:08:18
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Jonghun Lee
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Rutgers University. Graduate School - New Brunswick
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Author Agreement License
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I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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