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Tuneable collagen scaffolds for the directed differentiation of embryonic stem cells toward neural lineages

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TypeOfResource
Text
TitleInfo (ID = T-1)
Title
Tuneable collagen scaffolds for the directed differentiation of embryonic stem cells toward neural lineages
SubTitle
PartName
PartNumber
NonSort
Identifier (displayLabel = ); (invalid = )
ETD_2420
Identifier (type = hdl)
http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000052137
Language (objectPart = )
LanguageTerm (authority = ISO639-2); (type = code)
eng
Genre (authority = marcgt)
theses
Subject (ID = SBJ-1); (authority = RUETD)
Topic
Biomedical Engineering
Subject (ID = SBJ-2); (authority = ETD-LCSH)
Topic
Tissue engineering
Subject (ID = SBJ-3); (authority = ETD-LCSH)
Topic
Embryonic stem cells--Differentiation
Abstract
This thesis aims to develop and understand the role of peptide modified collagen scaffolds in tissue engineering and stem cell differentiation. The extracellular matrix and microenvironment surrounding cells plays a critical role in controlling phenotypic and genotypic behavior of cells. It is important to elucidate this role in stem cell differentiation to develop regenerative therapies. Here in, we developed a method to alter, positively and negatively, the adhesion migration and reorganization of scaffolds by fibroblast and smooth muscle cells on and in collagen. By grafting an adhesive peptide 'RGD' and a scramble non-adhesive peptide 'RDG' to collagen we were able to modulate in a dose dependent manner the adhesion of cells to collagen and establish the bimodal dependence of migration on adhesion in and on collagen scaffolds. We then extended this approach to understand the role of cell adhesion on differentiation of stem cells; we assayed the differentiation of sox-1 reporting murine embryonic stem cells on these scaffolds. Sox-1 expression was found to be inversely related to cell-matrix adhesion. Sox-1 reporting stem cells cultured on RDG grafted collagen scaffolds had a significantly greater population of cells that were sox-1 positive relative to controls. However, the difference between conditions was at most 10%. To further evaluate the ability of matrix cues to guide the differentiation of stem cells we grafted peptide mimics of neural stem cell niche carbohydrates, human natural killer-1 and polysialic acid to collagen and assayed the differentiation of stem cells towards cell types from all three germ layers. Cells cultured on human natural killer-1 and polysialic acid grafted collagen preferentially differentiated towards neuroectoderm lineage cells as indicated by qPCR and Immunohistochemistry. Identifying matrix cues and environments that support and direct the differentiation of stem cell is essential for regenerative medicine. Scaffolds that support and direct the differentiation of stem cells may eventually be used as therapies for degenerative diseases.
PhysicalDescription
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electronic resource
Extent
xi, 94 p. : ill.
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application/pdf
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Note (type = degree)
Ph.D.
Note (type = bibliography)
Includes bibliographical references
Note (type = statement of responsibility)
by Gary Albert Monteiro
Name (ID = NAME-1); (type = personal)
NamePart (type = family)
Monteiro
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Gary Albert
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1980-
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author
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Gary Albert Monteiro
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Shreiber
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David
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chair
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Advisory Committee
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David I Shreiber
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Yarmush
NamePart (type = given)
Martin
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internal member
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Advisory Committee
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Martin Yarmush
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Cai
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Li
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internal member
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Advisory Committee
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Li Cai
Name (ID = NAME-5); (type = personal)
NamePart (type = family)
Foty
NamePart (type = given)
Ramsey
Role
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outside member
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Advisory Committee
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Ramsey Foty
Name (ID = NAME-1); (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB); (type = )
degree grantor
Name (ID = NAME-2); (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB); (type = )
school
OriginInfo
DateCreated (point = ); (qualifier = exact)
2010
DateOther (qualifier = exact); (type = degree)
2010-01
Place
PlaceTerm (type = code)
xx
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TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
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NjNbRU
Identifier (type = doi)
doi:10.7282/T3HQ402D
Genre (authority = ExL-Esploro)
ETD doctoral
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Rights

RightsDeclaration (AUTHORITY = GS); (ID = rulibRdec0006)
The author owns the copyright to this work.
Copyright
Status
Copyright protected
Notice
Note
Availability
Status
Open
Reason
Permission or license
Note
RightsHolder (ID = PRH-1); (type = personal)
Name
FamilyName
Monteiro
GivenName
Gary
Role
Copyright Holder
RightsEvent (ID = RE-1); (AUTHORITY = rulib)
Type
Permission or license
Label
Place
DateTime
2010-01-06 16:47:29
Detail
AssociatedEntity (ID = AE-1); (AUTHORITY = rulib)
Role
Copyright holder
Name
Gary Monteiro
Affiliation
Rutgers University. Graduate School - New Brunswick
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Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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ETD
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application/pdf
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application/x-tar
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