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Modeling the growth of Salmonella spp. on cut tomatoes
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Text
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Modeling the growth of Salmonella spp. on cut tomatoes
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ETD_2339
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http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000052140
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eng
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theses
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Food Science
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(authority = ETD-LCSH)
Topic
Salmonella
Subject
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(authority = ETD-LCSH)
Topic
Tomatoes
Abstract
Tomato-associated Salmonella outbreaks have recently become a significant food safety concern. Temperature abuse of cut tomatoes may have played a role in some of these outbreaks.
The purpose of this study was to develop a mathematical model to describe the growth of Salmonella on cut tomatoes at various temperatures.
Four Salmonella serotypes (Salmonella Typhimurium, Salmonella Newport, Salmonella Javiana, Salmonella Braenderup) obtained from previous tomato-linked cases of salmonellosis were used in this study. These four serotypes were cultured separately, combined into a cocktail and inoculated onto whole red round tomatoes and allowed to dry overnight. The tomatoes were then cut into pieces and incubated at a predetermined range of temperatures (10, 12.5, 15, 18.5, 20, 22.5, 25, 27.5, 30, and 35°C). Salmonella concentration was measured at specified time intervals to determine the growth curve for Salmonella on cut tomatoes at each temperature. The growth rates were calculated using DMFit and used to build a mathematical model to illustrate the relationship between the growth rates of Salmonella on tomatoes and incubation temperatures from 10-35°C.
The resulting model compared favorably with a Salmonella growth model for raw poultry developed by our laboratory. The Pathogen Modeling Program under-predicted growth at low temperatures and over predicted growth at high temperatures. ComBase predicted consistently slower growth rates than were observed in tomatoes, but showed parallel increases in growth rate with increasing temperature.
The purpose of this study was to develop a mathematical model to describe the growth of Salmonella on cut tomatoes at various temperatures.
Four Salmonella serotypes (Salmonella Typhimurium, Salmonella Newport, Salmonella Javiana, Salmonella Braenderup) obtained from previous tomato-linked cases of salmonellosis were used in this study. These four serotypes were cultured separately, combined into a cocktail and inoculated onto whole red round tomatoes and allowed to dry overnight. The tomatoes were then cut into pieces and incubated at a predetermined range of temperatures (10, 12.5, 15, 18.5, 20, 22.5, 25, 27.5, 30, and 35°C). Salmonella concentration was measured at specified time intervals to determine the growth curve for Salmonella on cut tomatoes at each temperature. The growth rates were calculated using DMFit and used to build a mathematical model to illustrate the relationship between the growth rates of Salmonella on tomatoes and incubation temperatures from 10-35°C.
The resulting model compared favorably with a Salmonella growth model for raw poultry developed by our laboratory. The Pathogen Modeling Program under-predicted growth at low temperatures and over predicted growth at high temperatures. ComBase predicted consistently slower growth rates than were observed in tomatoes, but showed parallel increases in growth rate with increasing temperature.
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electronic resource
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vi, 26 p. : ill.
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M.S.
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Includes bibliographical references (p. 23-26)
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by Wenjing Pan
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Pan
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Wenjing
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1982-
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Wenjing Pan
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Schaffner
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Donald
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chair
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Donald W Schaffner
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Matthews
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Karl
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Karl R Matthews
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Chikindas
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Michael
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Michael L Chikindas
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Rutgers University
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Graduate School - New Brunswick
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2010
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2010-01
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xx
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Rutgers University Electronic Theses and Dissertations
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ETD
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Graduate School - New Brunswick Electronic Theses and Dissertations
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rucore19991600001
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doi:10.7282/T3WW7HTR
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ETD graduate
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The author owns the copyright to this work.
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Copyright protected
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Open
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Pan
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Wenjing
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2009-12-23 22:39:54
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Wenjing Pan
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Rutgers University. Graduate School - New Brunswick
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I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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