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Investigation of novel NRF2 partners, RAC3 and IQGAP1

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TypeOfResource
Text
TitleInfo (ID = T-1)
Title
Investigation of novel NRF2 partners, RAC3 and IQGAP1
Identifier
ETD_1587
Identifier (type = hdl)
http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000052254
Language
LanguageTerm (authority = ISO639-2); (type = code)
eng
Genre (authority = marcgt)
theses
Subject (ID = SBJ-1); (authority = RUETD)
Topic
Pharmaceutical Science
Subject (ID = SBJ-2); (authority = ETD-LCSH)
Topic
Transcription factors
Subject (ID = SBJ-3); (authority = ETD-LCSH)
Topic
Cell receptors
Subject (ID = SBJ-4); (authority = ETD-LCSH)
Topic
Nuclear receptors (Biochemistry)
Abstract
Nuclear factor-erythroid-related factor 2 (Nrf2) is essential for the antioxidant responsive element (ARE)-mediated expression of a group of detoxifying and antioxidant genes, which detoxify carcinogens and protect against oxidative stress. In the current study, we investigated the novel Nrf2 partners RAC3/AIB1/SRC-3 and IQGAP1 on Nrf2 signaling. Here, we found that overexpression of RAC3, a nuclear co-regulator, induced Heme oxygenase-1 through Nrf2 transactivation in HeLa cells. Next, we conducted the interaction study between the RAC3 and Nrf2 proteins using co-immunoprecipitation assay (Co-IP) and Fluorescence Resonance Energy Transfer (FRET) analysis. The results showed that RAC3 bound to Nrf2 protein directly in the nucleus. Subsequently, we identified the domains of Nrf2 and RAC3 of their interaction using GST-pull down assay. The results showed that both N-terminal RAC-pas B and C-terminal RAC3-R3B3 were tightly bound to Neh4 and Neh5 transactivation domains. Furthermore, chromatin immunoprecipitation (ChIP) showed that RAC3 tightly bound to the ARE enhancer region of the HO-1 promoter via Nrf2 binding. The data suggested that Nrf2 activation can be modulated and directly controlled via interacts with RAC3 protein in the HeLa cells. Next, we investigated IQGAP1, a calmodulin binding protein, as a novel Nrf2 partner isolated using One-strep tag pull-down method in HeLa cells. Initially, we tested the effect of calcium on Nrf2/ARE-lucifease activity and heme oxygenase-1 (HO-1) protein induction in HeLa cells. The results showed that Nrf2/ARE-lucifease activity and expression of HO-1 were increased by treatment of 3.8 mM of CaCl2, suggesting calcium may be one of the important factors in Nrf2 signaling. Next, we investigated a function of IQGAP1 on Nrf2 signaling by co-transfection with EGFP-Nrf2 and Dsredm-IQGAP1 constructs in HeLa cells. The results showed that Dsredm-IQGAP1 transfection strongly increased the HO-1 expression and the stability of EGFP-Nrf2, which was abolished by siIQGAP1. Furthermore, IP study showed that the Nrf2-IQGAP1 complex was disrupted by CaCl2, treatment suggesting Nrf2 might be liberated from IQGAP1 protein by calcium to be an active form. These results inferred that IQGAP1 may play a pivotal role in Nrf2 signaling in conjunction with intracellular calcium level. Taken together, our results suggest that both RAC3 and IQGAP1 may play an important role in Nrf2 transactivation and its signaling pathway, respectively.
PhysicalDescription
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electronic resource
Extent
xv, 152 p. : ill.
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application/pdf
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text/xml
Note (type = degree)
Ph.D.
Note (type = bibliography)
Includes bibliographical references (p. 135-149)
Note (type = statement of responsibility)
by Jung-Hwan Kim
Name (ID = NAME-1); (type = personal)
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Kim
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Jung-Hwan
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author
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Jung-Hwan Kim
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Kong
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Ah-Ng Tony
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chair
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Advisory Committee
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Ah-Ng Tony Kong
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Chen
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Suzie
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internal member
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Advisory Committee
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Suzie Chen
Name (ID = NAME-4); (type = personal)
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Suh
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Nanjoo
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internal member
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Advisory Committee
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Nanjoo Suh
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NamePart (type = family)
Chen
NamePart (type = given)
J. Don
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outside member
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Advisory Committee
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J. Don Chen
Name (ID = NAME-1); (type = corporate)
NamePart
Rutgers University
Role
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degree grantor
Name (ID = NAME-2); (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB)
school
OriginInfo
DateCreated (qualifier = exact)
2009
DateOther (qualifier = exact); (type = degree)
2009
Place
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xx
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NjNbRU
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TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Identifier (type = doi)
doi:10.7282/T33X86S5
Genre (authority = ExL-Esploro)
ETD doctoral
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The author owns the copyright to this work.
Copyright
Status
Copyright protected
Notice
Note
Availability
Status
Open
Reason
Permission or license
Note
RightsHolder (ID = PRH-1); (type = personal)
Name
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Kim
GivenName
Jung-Hwan
Role
Copyright Holder
RightsEvent (AUTHORITY = rulib); (ID = RE-1)
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Place
DateTime
2009-04-01 14:50:57
Detail
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Name
Jung-Hwan Kim
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject (AUTHORITY = rulib); (ID = AO-1)
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Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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365 days
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Technical

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ETD
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application/pdf
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