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Regulation of endocytic recycling in Caenorhabditis elegans
Descriptive
TypeOfResource
Text
TitleInfo
(ID = T-1)
Title
Regulation of endocytic recycling in Caenorhabditis elegans
Identifier
ETD_2497
Identifier
(type = hdl)
http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000053159
Language
LanguageTerm
(authority = ISO639-2);
(type = code)
eng
Genre
(authority = marcgt)
theses
Subject
(ID = SBJ-1);
(authority = RUETD)
Topic
Microbiology and Molecular Genetics
Subject
(ID = SBJ-2);
(authority = ETD-LCSH)
Topic
Endocytosis
Subject
(ID = SBJ-3);
(authority = ETD-LCSH)
Topic
Caenorhabditis elegans
Abstract
(type = abstract)
Eukaryotic endocytic pathway is important for the uptake, sorting, and the subsequential recycling or degradation processes of various cargos. It has been shown that the RME-1/EHD1 is a critical regulator of endocytic recycling. In C. elegans and MDCK cells, small GTPase RAB-10 has been specifically implicated in clathrin-independent cargo recycling. Alternatively, some cargos will be recycled from sorting endosomes to Golgi, and retromer complex was shown to be crucial for this transport in yeast and mammalian cells. In our studies, we analyzed RME-1 and RAB-10 regulated recycling pathway, exploring additional players in the process. First, we demonstrated that ALX-1 is required for endocytic recycling of specific basolateral cargo. The interaction of ALX-1 with RME-1 is required for this recycling process. In our yeast two-hybrid screen for RAB-10-interacting proteins, EHBP-1 and Arf6 GAP/CNT-1 were recovered and revealed to function together with RAB-10 regulating clathrin-independent cargo recycling. Loss of either EHBP-1 or CNT-1 produced rab-10-like cargo transport defects. Furthermore, we showed that EHBP-1 functions, as an unconventional effector, upstream of RAB-10. Nevertheless, similar to canonical Rab effectors, CNT-1 requires RAB-10 for the proper endosome localization. Collectively, our results demonstrate the functional connections of ALX-1/RME-1 and RAB-10/EHBP-1/CNT-1, provided insights into the detail mechanisms of RME-1 and RAB-10 recycling regulation. We also studied the retromer regulated retrograde transport in C. elegans. We demonstrated the physical interaction of RME-8 with retromer component ALX-1. Additionally, we showed that loss-of-function in rme-8 or snx-1, or depletion of C. elegans Hsc70 (HSP-1) by RNAi, disrupts endosome to Golgi transport of the retromer-dependent cargo protein MIG-14. Furthermore, we identified a previously unsuspected mechanism for the regulation of endosomal clathrin that is required for retrograde transport. We showed that loss of either RME-8, SNX-1 or HSP-1 leads to endosomal clathrin dynamics defect. Our work indicates that, through RME-8 and Hsc70, the retromer acts to limit clathrin accumulation, a prerequisite for the recycling of retrograde cargo.
PhysicalDescription
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electronic resource
Extent
x, 240 p. : ill.
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application/pdf
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text/xml
Note
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Ph.D.
Note
Includes abstract
Note
Vita
Note
(type = bibliography)
Includes bibliographical references
Note
(type = statement of responsibility)
by Anbing Shi
Name
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Shi
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Anbing
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1974-
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author
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Anbing Shi
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Padgett
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Richard W
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chair
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Advisory Committee
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Richard W Padgett
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(type = family)
Grant
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Barth D
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internal member
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Advisory Committee
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Barth D Grant
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Rongo
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Christopher
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Advisory Committee
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Christopher Rongo
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Lobel
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Peter
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outside member
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Advisory Committee
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Peter Lobel
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Rutgers University
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degree grantor
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Graduate School - New Brunswick
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school
OriginInfo
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2010
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2010
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xx
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NjNbRU
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Title
Rutgers University Electronic Theses and Dissertations
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ETD
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Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier
(type = local)
rucore19991600001
Identifier
(type = doi)
doi:10.7282/T33J3D2J
Genre
(authority = ExL-Esploro)
ETD doctoral
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Rights
RightsDeclaration
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The author owns the copyright to this work.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
RightsHolder
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Name
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Shi
GivenName
Anbing
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Permission or license
DateTime
2010-03-18 18:55:17
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Anbing Shi
Affiliation
Rutgers University. Graduate School - New Brunswick
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Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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DateTime
2012-05-30
Detail
730 days
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Copyright holder
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Anbing Shi
Affiliation
Rutgers University. Graduate School - New Brunswick
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Type
License
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Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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Technical
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ETD
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application/pdf
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application/x-tar
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8181760
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