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Identification and expression of the cytochrome P450 reductase gene in Aedes sollicitans (Walker) (Diptera: culicidae)
Descriptive
TypeOfResource
Text
TitleInfo
(ID = T-1)
Title
Identification and expression of the cytochrome P450 reductase gene in Aedes sollicitans (Walker) (Diptera: culicidae)
Identifier
ETD_2629
Identifier
(type = hdl)
http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000053216
Language
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(authority = ISO639-2);
(type = code)
eng
Genre
(authority = marcgt)
theses
Subject
(ID = SBJ-1);
(authority = RUETD)
Topic
Entomology
Subject
(ID = SBJ-2);
(authority = ETD-LCSH)
Topic
Cytochrome P-450
Subject
(ID = SBJ-3);
(authority = ETD-LCSH)
Topic
Mosquitoes as carriers of disease
Abstract
(type = abstract)
Aedes sollicitans (Walker) is an important vector of mosquito-borne diseases. Cytochrome P450 system is one of the most important enzyme families involved in the metabolic responses of living organisms to foreign chemicals. To explore the specific functions of individual cytochrome P450s, it is important to identify the cytochrome P450 reductase gene. The cytochrome P450 reductase genes were identified in Ae. sollicitans from Cape May, Middlesex and Ocean County, New Jersey. The cytochrome P450 reductase gene has an open reading frame of 2040 bp encoding 679 amino acid residues. The comparison among 679 amino acid residues deduced from the cytochrome P450 reductase gene obtained from three mosquito populations showed that mutations had occurred at the nucleotide level but did not produce amino acid changes except in one case. In Ocean County mosquito population, the amino acid serine was changed to glycine. The amino acid change was not considered to cause any functional change of cytochrome P450 reductase since the mutation was not located in any functional region. Quantitative real-time expression analysis showed that the expressions of CYP6BB1 and cytochrome P450 reductase mRNAs were not significantly different in fourth instar larvae or in different body parts of adult female mosquitoes in populations from three locations. The study implied that CYP6BB1 was ubiquitously expressed in female mosquitoes and its expression occurred in both larvae and adults. The cytochrome P450 reductase gene was expressed in E. coli cells and molecular mass of the protein was estimated to be 78 kDa. Its activity was assayed in both the cytosolic and cell membrane fractions. The activity in the cytosol was much higher than that in the membrane fractions. The cytochrome P450 reductase activities in microsomes of Ae. sollicitans collected from the three locations were assayed. The results showed no statistically significant differences. This is the first study of cytochrome P450 reductase in Ae. sollicitans. The identification and expression of the cytochrome P450 reductase gene provide further insights on how cytochrome P450s metabolize substrates and make it possible to explore the specific functions of each cytochrome P450 gene. The study is not only meaningful for Ae. sollicitans but also for other mosquito species.
PhysicalDescription
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electronic resource
Extent
ix, 80 p. : ill.
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application/pdf
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text/xml
Note
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Ph.D.
Note
Includes abstract
Note
Vita
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(type = bibliography)
Includes bibliographical references
Note
(type = statement of responsibility)
by Debin Sun
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Sun
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Debin
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author
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DEBIN SUN
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Brattsten
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Lena B
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chair
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Advisory Committee
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Lena B Brattsten
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(type = family)
Hamilton
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(type = given)
George C
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internal member
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Advisory Committee
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George C Hamilton
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NamePart
(type = family)
Koppenhöfer
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Albrecht
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internal member
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Advisory Committee
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Albrecht Koppenhöfer
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NamePart
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Thomas
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(type = given)
Paul E
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outside member
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Advisory Committee
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Paul E Thomas
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NamePart
Rutgers University
Role
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degree grantor
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Graduate School - New Brunswick
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school
OriginInfo
DateCreated
(qualifier = exact)
2010
DateOther
(qualifier = exact);
(type = degree)
2010
Place
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(type = code)
xx
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TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
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ETD
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(type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
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rucore19991600001
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NjNbRU
Identifier
(type = doi)
doi:10.7282/T3C24WJN
Genre
(authority = ExL-Esploro)
ETD doctoral
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Rights
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The author owns the copyright to this work.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
RightsHolder
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(type = personal)
Name
FamilyName
SUN
GivenName
DEBIN
Role
Copyright Holder
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(AUTHORITY = rulib)
Type
Permission or license
DateTime
2010-04-15 12:27:14
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Name
DEBIN SUN
Affiliation
Rutgers University. Graduate School - New Brunswick
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Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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Technical
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ETD
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application/pdf
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application/x-tar
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3461120
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