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Albumin-derived nanoparticles

Descriptive

TypeOfResource
Text
TitleInfo (ID = T-1)
Title
Albumin-derived nanoparticles
SubTitle
a flexible biointerface for enhanced adhesion, spatial guidance and growth factor stimulation of human mesenchymal stem cells
Identifier
ETD_2606
Identifier (type = hdl)
http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000053222
Language
LanguageTerm (authority = ISO639-2); (type = code)
eng
Genre (authority = marcgt)
theses
Subject (ID = SBJ-1); (authority = RUETD)
Topic
Cell and Developmental Biology
Subject (ID = SBJ-2); (authority = ETD-LCSH)
Topic
Stem cells
Subject (ID = SBJ-3); (authority = ETD-LCSH)
Topic
Wound healing
Subject (ID = SBJ-4); (authority = ETD-LCSH)
Topic
Nanoparticles
Abstract (type = abstract)
Mesenchymal stem cells (MSCs) are multipotent stem cells that can differentiate into a variety of cell types and have been widely investigated as a possible alternative treatment strategy for wound healing. A major challenge to the direct application of MSCs in severe wounds is the limited engraftment of these cells at the wound sites (<1%) and a lack of spatial control of MSC adhesion and activation, which could be potentially enhanced through engineered nanoscale multifunctional substrates. The use of human albumin-derived nanoparticles (ANPs) as a foundational nanoscale substrate to display RGD-containing fibronectin FIII9-10 fragment (Abbreviated: Fnf) has been previously reported in the Moghe laboratory. When presented in 2-D cell cultures on ANPs, this ligand has been proven to promote cell motility and extracellular matrix assembly. Here we showed Fnf-ANPs promoted human MSCs (hMSCs) adhesion as well as integrin clustering in cell protrusions and were able to spatially direct the adhesion and growth of hMSC on 2-D polymer substrates. Furthermore, hMSC morphology and its organization of focal adhesion were sensitively modulated by nanoscale size of ANPs. The adhesion of hMSC was cooperatively promoted in the presence of Fnf-ANPs and growth factor, bFGF. In order to promote similar synergies between the biofunctionalized ANPs and growth factors, we engineered albumin nanoparticles with P14 peptide, a fibronectin-derived growth factor sequestering peptide proven to bind to a variety of growth factors. Thus, we demonstrate that albumin-derived nanoparticles can be used as a flexible system for nanoscale presentation of adhesion and growth factors, the combination of which could potentially control hMSC phenotype and functions.
PhysicalDescription
Form (authority = gmd)
electronic resource
Extent
ix, 72 p. : ill.
InternetMediaType
application/pdf
InternetMediaType
text/xml
Note (type = degree)
M.S.
Note
Includes abstract
Note
Vita
Note (type = bibliography)
Includes bibliographical references
Note (type = statement of responsibility)
by Jing Xu
Name (ID = NAME-1); (type = personal)
NamePart (type = family)
Xu
NamePart (type = given)
Jing
NamePart (type = date)
1980-
Role
RoleTerm (authority = RULIB)
author
DisplayForm
Jing Xu
Name (ID = NAME-2); (type = personal)
NamePart (type = family)
Moghe
NamePart (type = given)
Prabhas
Role
RoleTerm (authority = RULIB)
chair
Affiliation
Advisory Committee
DisplayForm
Prabhas Moghe
Name (ID = NAME-3); (type = personal)
NamePart (type = family)
Cai
NamePart (type = given)
Li
Role
RoleTerm (authority = RULIB)
internal member
Affiliation
Advisory Committee
DisplayForm
Li Cai
Name (ID = NAME-4); (type = personal)
NamePart (type = family)
Foty
NamePart (type = given)
Ramsey
Role
RoleTerm (authority = RULIB)
internal member
Affiliation
Advisory Committee
DisplayForm
Ramsey Foty
Name (ID = NAME-1); (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (ID = NAME-2); (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB)
school
OriginInfo
DateCreated (qualifier = exact)
2010
DateOther (qualifier = exact); (type = degree)
2010
Place
PlaceTerm (type = code)
xx
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T34J0F6X
Genre (authority = ExL-Esploro)
ETD graduate
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Rights

RightsDeclaration (AUTHORITY = GS); (ID = rulibRdec0006)
The author owns the copyright to this work.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
RightsHolder (ID = PRH-1); (type = personal)
Name
FamilyName
Xu
GivenName
Jing
Role
Copyright Holder
RightsEvent (ID = RE-1); (AUTHORITY = rulib)
Type
Permission or license
DateTime
2010-04-14 15:58:02
AssociatedEntity (ID = AE-1); (AUTHORITY = rulib)
Role
Copyright holder
Name
Jing Xu
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject (ID = AO-1); (AUTHORITY = rulib)
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent (ID = RE-2); (AUTHORITY = rulib)
Type
Embargo
DateTime
2010-05-31
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after November 30th, 2010.
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Technical

ContentModel
ETD
MimeType (TYPE = file)
application/pdf
MimeType (TYPE = container)
application/x-tar
FileSize (UNIT = bytes)
2027520
Checksum (METHOD = SHA1)
334ca252cea0f283979b75c89d038d14d05a8b6e
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