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Checkpoint-mediated regulations of meiotic double-strand-break repair and crossover formation in Drosophila melanogaster
Descriptive
TypeOfResource
Text
TitleInfo
(ID = T-1)
Title
Checkpoint-mediated regulations of meiotic double-strand-break repair and crossover formation in Drosophila melanogaster
Identifier
ETD_2532
Identifier
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http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000053280
Language
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eng
Genre
(authority = marcgt)
theses
Subject
(ID = SBJ-1);
(authority = RUETD)
Topic
Microbiology and Molecular Genetics
Subject
(ID = SBJ-2);
(authority = ETD-LCSH)
Topic
Meiosis
Subject
(ID = SBJ-3);
(authority = ETD-LCSH)
Topic
Drosophila melanogaster--Genetics
Subject
(ID = SBJ-4);
(authority = ETD-LCSH)
Topic
Genetic recombination--Research
Abstract
(type = abstract)
During meiotic prophase, programmed DNA double-strand breaks (DSBs) are required to initiate recombination between homologous chromosomes and are repaired as either crossovers or noncrossovers. Crossovers provide a link between the homologs and facilitate their orientation on the meiosis I spindle and segregation at anaphase. Although studies in a variety of experiment systems have identified and characterized numerous factors necessary for crossover formation, far less is known about how the distribution and number of crossovers are controlled. The process of repairing meiotic DSBs is monitored by at least two surveillance mechanisms: the canonical DSB repair checkpoint that responds to DNA damage and another that requires the widely conserved AAA+ ATPase Pch2, hereafter referred to as the "pachytene checkpoint." In Saccharomyces cerevisiae and Caenorhabditis elegans, pachytene checkpoint activity has been detected with mutations in genes encoding structural proteins required for crossing over; however, it remains unclear what the underlying process is that the pachytene checkpoint is monitoring. I identified the Drosophila pachytene checkpoint by conducting a phenotypic analysis of different classes of recombination-defective mutants. Specifically, the Drosophila pachytene checkpoint delays prophase progression when genes necessary for DSB repair and crossover formation are defective, but surprisingly functions independently of DSB formation. In addition, I investigated the relationship between chromosome structure and the pachytene checkpoint and found that defects in chromosome axis components also cause pch2-dependent delays. These findings suggest the pachytene checkpoint monitors two genetically distinct events: an early function of DSB repair proteins and the organization of chromosome axes. In support of this model, heterozygous chromosome aberrations result in a pachytene delay and interchromosomal increase in crossovers that are both dependent on pch2. My studies have led to a model where the sites and/or conditions required to promote crossovers are established independent of DSB formation early in meiotic prophase. The pachytene checkpoint may function to promote an optimal number of crossovers by regulating the duration of PCH2 expression, which defines the crossover determination phase. These results have provided new insights into this highly conserved surveillance mechanism as well as its relationship to pachytene progression and crossover control.
PhysicalDescription
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electronic resource
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xv, 170 p. : ill.
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application/pdf
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text/xml
Note
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Ph.D.
Note
Includes abstract
Note
Vita
Note
(type = bibliography)
Includes bibliographical references
Note
(type = statement of responsibility)
by Eric F. Joyce
Name
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Joyce
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Eric F.
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1982-
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author
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Eric Joyce
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Steward
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Ruth
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chair
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Advisory Committee
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Ruth Steward
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NamePart
(type = family)
Tischfield
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(type = given)
Jay
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internal member
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Advisory Committee
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Jay Tischfield
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Walworth
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(type = given)
Nancy
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outside member
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Advisory Committee
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Nancy Walworth
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Rutgers University
Role
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degree grantor
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Graduate School - New Brunswick
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school
OriginInfo
DateCreated
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2010
DateOther
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(type = degree)
2010
Place
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xx
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TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
(type = RULIB)
ETD
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(type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier
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rucore19991600001
Location
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NjNbRU
Identifier
(type = doi)
doi:10.7282/T35H7GBR
Genre
(authority = ExL-Esploro)
ETD doctoral
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Rights
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(ID = rulibRdec0006)
The author owns the copyright to this work.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
RightsHolder
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(type = personal)
Name
FamilyName
Joyce
GivenName
Eric
Role
Copyright Holder
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(AUTHORITY = rulib)
Type
Permission or license
DateTime
2010-04-05 14:38:47
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Copyright holder
Name
Eric Joyce
Affiliation
Rutgers University. Graduate School - New Brunswick
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License
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Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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Technical
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ETD
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application/pdf
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application/x-tar
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7567360
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