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The effect of MGAT expression on cellular lipid metabolism

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TypeOfResource
Text
TitleInfo (ID = T-1)
Title
The effect of MGAT expression on cellular lipid metabolism
Identifier
ETD_2794
Identifier (type = hdl)
http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000056145
Language
LanguageTerm (authority = ISO639-2); (type = code)
eng
Genre (authority = marcgt)
theses
Subject (ID = SBJ-1); (authority = RUETD)
Topic
Nutritional Sciences
Subject (ID = SBJ-2); (authority = ETD-LCSH)
Topic
Lipids--Synthesis
Subject (ID = SBJ-3); (authority = ETD-LCSH)
Topic
Lipids in human nutrition
Subject (ID = SBJ-4); (authority = ETD-LCSH)
Topic
Lipids--Metabolism
Abstract
Following absorption into the enterocyte, free fatty acid (FFA) and sn-2-monoacylglycerol (sn-2-MG), the major hydrolysis products of dietary lipids, are reconstituted to triacylglycerol (TG) by the monoacylglycerol acyltransferase (MGAT) pathway, which is the primary TG synthesis pathway in the postprandial condition. Nevertheless, the precise function and contribution of the MGAT pathway to lipid synthesis are not fully understood. Therefore in this study, we examined the effect of MGAT expression on cellular lipid metabolism. Although the Caco-2 cell line, which is derived from human colorectal cancer cells, is the most relevant in vitro model to examine the influence of hMGAT2 expression on lipid metabolism in the enterocyte, we could not obtain any definitive metabolism data caused by MGAT expression due to their inconsistent transfection efficiency. Thus we transiently expressed hMGAT2 in CHO-K1 cells, which are relatively easier to transfect, and these cells were then used to study the metabolism of radiolabeled FFA or sn-2-MG. Empty vector-transfected (mock) and MGAT-transfected cells, which were incubated with FA, showed the increased net uptake as a function of time, and both groups metabolized most of absorbed FA to phospholipid (PL). Between the two groups, there were no significant differences. In contrast, MGAT expressing cells which were incubated with MG, absorbed remarkably higher amounts of lipid relative to mock-transfected cells. This suggests that MGAT expression promotes the rapid uptake of MG across the membrane by efficiently maintaining concentration gradients, or that the transmembrane lipid transport proteins are influenced by MGAT expression. The incorporation of absorbed MG into each lipid class was also significantly different between mock and MGAT expressing cells. More MG was incorporated into TG and DG in the MGAT transfected cells. In addition, the MGAT expressing cells showed higher percent incorporation into PL and lower percent incorporation into TG comparing to mock expressing cells. This difference in metabolic channeling suggests that diacylglycerol (DG) synthesized from MGAT pathway may not be equivalent with DG synthesized from G-3-P pathway, which is the ubiquitous metabolic pathway used to synthesize TG in most cells. We therefore suggest that MGAT expression may increase the net uptake of lipid into cells, and that the G-3-P and MGAT pathways may have different pools of DG which are metabolized to PL or TG.
PhysicalDescription
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electronic resource
Extent
xii, 74 p. : ill.
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application/pdf
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text/xml
Note (type = degree)
M.S.
Note (type = bibliography)
Includes bibliographical references
Note (type = statement of responsibility)
by Kyong Jin Ahn
Name (ID = NAME-1); (type = personal)
NamePart (type = family)
Ahn
NamePart (type = given)
Kyong Jin
NamePart (type = date)
1981-
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author
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Kyong Jin Ahn
Name (ID = NAME-2); (type = personal)
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Storch
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Judith
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chair
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Advisory Committee
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Judith Storch
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NamePart (type = family)
Brasaemle
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Dawn L
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internal member
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Advisory Committee
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Dawn L Brasaemle
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NamePart (type = family)
Igal
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Ariel
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internal member
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Advisory Committee
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Ariel Igal
Name (ID = NAME-5); (type = personal)
NamePart (type = family)
Igal
NamePart (type = given)
R.Ariel
Role
RoleTerm (authority = RULIB)
internal member
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Advisory Committee
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R.Ariel Igal
Name (ID = NAME-1); (type = corporate)
NamePart
Rutgers University
Role
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degree grantor
Name (ID = NAME-2); (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB)
school
OriginInfo
DateCreated (qualifier = exact)
2010
DateOther (qualifier = exact); (type = degree)
2010-10
Place
PlaceTerm (type = code)
xx
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
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NjNbRU
Identifier (type = doi)
doi:10.7282/T3862G7B
Genre (authority = ExL-Esploro)
ETD graduate
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RightsDeclaration (AUTHORITY = GS); (ID = rulibRdec0006)
The author owns the copyright to this work.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
RightsHolder (ID = PRH-1); (type = personal)
Name
FamilyName
Ahn
GivenName
Kyong Jin
Role
Copyright Holder
RightsEvent (ID = RE-1); (AUTHORITY = rulib)
Type
Permission or license
DateTime
2010-07-30 15:22:51
AssociatedEntity (ID = AE-1); (AUTHORITY = rulib)
Role
Copyright holder
Name
Kyong Jin Ahn
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject (ID = AO-1); (AUTHORITY = rulib)
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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ETD
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application/pdf
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application/x-tar
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