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Factors that define the developmental program and trajectory of the porcine uterus
Descriptive
TypeOfResource
Text
TitleInfo
(ID = T-1)
Title
Factors that define the developmental program and trajectory of the porcine uterus
Identifier
ETD_2920
Identifier
(type = hdl)
http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000056212
Language
LanguageTerm
(authority = ISO639-2);
(type = code)
eng
Genre
(authority = marcgt)
theses
Subject
(ID = SBJ-1);
(authority = RUETD)
Topic
Animal Sciences
Subject
(ID = SBJ-2);
(authority = ETD-LCSH)
Topic
Swine--Growth
Subject
(ID = SBJ-3);
(authority = ETD-LCSH)
Topic
Uterus--Growth
Subject
(ID = SBJ-4);
(authority = ETD-LCSH)
Topic
Gene expression
Abstract
(type = abstract)
The early days of life represent an important period for neonatal uterine development in the pig. The porcine uterus develops in utero and continues postnatally. Events during neonatal life that establish uterine histoarchitecture contribute to the developmental program of the uterus and set the trajectory for adult uterine phenotype. Uterine programming is sensitive to hormonal perturbations. Exposure to bioactive factors during neonatal life influences uterine maturation with long-term consequences for reproductive performance. The goal of this research was to study factors in the neonatal environment that alter uterine developmental gene expression. These include mediators of growth [estrogen receptor alpha (ESR1), vascular endothelial growth factor (VEGFA) and relaxin receptor (RXFP1)], patterning (WNT7A and HOXA10) and remodeling [matrix metalloproteinase (MMP)9 and 2]. Results indicated that exposure to estradiol valerate (EV) from birth (postnatal day [PND] 0) to PND 14 increased uterine ESR1 and VEGFA protein, along with HOXA10, RXFP1 and MMP9 transcripts and decreased WNT7A transcripts, in PND 14 neonates. Endometrial gene expression changes also occured in adulthood on day 12 of pregnancy as a consequence of neonatal EV exposure. This included decreases in WNT7A and MMP9 transcripts, ESR1 and VEGFA protein and reduced MMP9 activity. Next, exposing gilts perinatally to the estrogenic mycotoxin zearalenone (ZEA), decreased uterine ESR1, WNT7A and RXFP1 mRNA levels on PND 21 compared to unexposed gilts. Extending the concept that postnatal uterine development in gilts can be influenced by maternally-derived signals, studies showed that nursing from birth supported uterine ESR1, VEGFA and MMP9 protein expression at PND 2 and PND 14 in comparison to milk replacer-fed gilts. Treatment with relaxin, an uterotrophic milk-borne hormone, did not restore the uterine phenotype of replacer-fed animals to that of nursing gilts at PND 2, although it did predictably affect RXFP1 transcript levels. Results support the idea that nursing during a critical two-day period from birth supports the neonatal porcine uterine developmental program. Identification of mediators that regulate neonatal uterine programming, the time frame for this regulation and understanding how those mediators are influenced by environmental/lactocrine factors provide critical insights into the mechanisms that govern neonatal tissue development.
PhysicalDescription
Form
(authority = gmd)
electronic resource
Extent
xii, 174 p. : ill.
InternetMediaType
application/pdf
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text/xml
Note
(type = degree)
Ph.D.
Note
(type = bibliography)
Includes bibliographical references
Note
(type = vita)
Includes vita
Note
(type = statement of responsibility)
by Joseph C. Chen
Name
(ID = NAME-1);
(type = personal)
NamePart
(type = family)
Chen
NamePart
(type = given)
Joseph C.
NamePart
(type = date)
1978-
Role
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(authority = RULIB)
author
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Joseph Chen
Name
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NamePart
(type = family)
Bagnell
NamePart
(type = given)
Carol A.
Role
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(authority = RULIB)
chair
Affiliation
Advisory Committee
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Carol A. Bagnell
Name
(ID = NAME-3);
(type = personal)
NamePart
(type = family)
Katz
NamePart
(type = given)
Larry S.
Role
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(authority = RULIB)
internal member
Affiliation
Advisory Committee
DisplayForm
Larry S. Katz
Name
(ID = NAME-4);
(type = personal)
NamePart
(type = family)
Uzumcu
NamePart
(type = given)
Mehmet
Role
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(authority = RULIB)
internal member
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Advisory Committee
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Mehmet Uzumcu
Name
(ID = NAME-5);
(type = personal)
NamePart
(type = family)
Bartol
NamePart
(type = given)
Frank F.
Role
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(authority = RULIB)
outside member
Affiliation
Advisory Committee
DisplayForm
Frank F. Bartol
Name
(ID = NAME-1);
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NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
(ID = NAME-2);
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NamePart
Graduate School - New Brunswick
Role
RoleTerm
(authority = RULIB)
school
OriginInfo
DateCreated
(qualifier = exact)
2010
DateOther
(qualifier = exact);
(type = degree)
2010-10
Place
PlaceTerm
(type = code)
xx
RelatedItem
(type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
(type = RULIB)
ETD
RelatedItem
(type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier
(type = local)
rucore19991600001
Location
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(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T3FX797Z
Genre
(authority = ExL-Esploro)
ETD doctoral
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Rights
RightsDeclaration
(AUTHORITY = GS);
(ID = rulibRdec0006)
The author owns the copyright to this work.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
RightsHolder
(ID = PRH-1);
(type = personal)
Name
FamilyName
Chen
GivenName
Joseph
Role
Copyright Holder
RightsEvent
(ID = RE-1);
(AUTHORITY = rulib)
Type
Permission or license
DateTime
2010-09-27 23:11:16
AssociatedEntity
(ID = AE-1);
(AUTHORITY = rulib)
Role
Copyright holder
Name
Joseph Chen
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
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(AUTHORITY = rulib)
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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Technical
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ETD
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(TYPE = file)
application/pdf
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application/x-tar
FileSize
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1187840
Checksum
(METHOD = SHA1)
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