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Analysis of the initiation of acentrosomal spindle assembly in Drosophila melanogaster
Descriptive
TypeOfResource
Text
TitleInfo
(ID = T-1)
Title
Analysis of the initiation of acentrosomal spindle assembly in Drosophila melanogaster
Identifier
ETD_2791
Identifier
(type = hdl)
http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000056208
Language
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eng
Genre
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theses
Subject
(ID = SBJ-1);
(authority = RUETD)
Topic
Microbiology and Molecular Genetics
Subject
(ID = SBJ-2);
(authority = ETD-LCSH)
Topic
Drosophila melanogaster
Subject
(ID = SBJ-3);
(authority = ETD-LCSH)
Topic
Spindle (Cell division)
Subject
(ID = SBJ-4);
(authority = ETD-LCSH)
Topic
Microtubules
Abstract
(type = abstract)
In many species, meiotic spindles assemble microtubules in the absence of centrosomes. An acentrosomal mechanism has been proposed where the chromosomes initiate spindle assembly by nucleating and capturing microtubules. While preliminary evidence has supported this model in Drosophila, very little is actually known about how a bipolar spindle is initiated and maintained in the absence of centrosomes. RanGTP is a small GTPase which has been shown to be important for chromosome-dependent spindle assembly in Xenopus extracts. I investigated the effect of manipulating the Ran pathway on spindle assembly in Drosophila oocytes and embryos. Surprisingly, I found that RanGTP is not essential for the formation of the acentrosomal spindle near the chromosomes. However, I discovered that RanGTP is essential for completing chromosome-independent microtubule assembly, such as pro-nuclear fusion. Thus, RanGTP may be required for microtubule assembly when chromosomes are absent, but redundant with the chromosomal passenger complex when chromosomes are present. Another contributor to acentrosomal spindle assembly is Subito, a member of the kinesin 6 superfamily. Subito binds and bundles microtubules at the central spindle in both centrosomal and acentrosomal systems. We have previously shown that expression of Subito with an N-terminus deletion results in the formation of chromosome-independent spindles, or ectopic spindles, in the Drosophila oocyte. By continuing the mutational analysis within the N-terminus, I have further characterized its regulatory role. We have shown that two conserved serines negatively regulate the motor activity of Subito, while other domains within the N-terminus are essential for positive regulation. So while originally believed to be simply a negative regulator, the N-terminus is actually a complex region of negative and positive regulators. My studies have helped to clarify the requirements for the initiation of acentrosomal spindle assembly in Drosophila. RanGTP is essential for spindle assembly in a chromosome independent manner, suggesting that chromosomal passenger complex is necessary for chromosome dependent microtubule assembly. Furthermore, we suggest a model of auto-inhibition for Subito with the N-terminus interacting with the C-terminus, preventing the binding of microtubules. Together, these results have provided new insights into acentrosomal spindle assembly.
PhysicalDescription
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electronic resource
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xv, 156 p. : ill.
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text/xml
Note
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Ph.D.
Note
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Includes bibliographical references
Note
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Includes vita
Note
(type = statement of responsibility)
by Jeffry Cesario
Name
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Cesario
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Jeffry
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1981-
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author
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Jeffry Cesario
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Vershon
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Andrew K.
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chair
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Advisory Committee
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Andrew K. Vershon
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McKim
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Kim S.
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internal member
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Advisory Committee
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Kim S. McKim
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Barr
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Maureen M.
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internal member
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Advisory Committee
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Maureen M. Barr
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Konsolaki
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Maria
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internal member
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Advisory Committee
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Maria Konsolaki
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Kusch
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Thomas N.
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outside member
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Advisory Committee
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Thomas N. Kusch
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Rutgers University
Role
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degree grantor
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Graduate School - New Brunswick
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school
OriginInfo
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2010
DateOther
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2010-10
Place
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xx
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Title
Rutgers University Electronic Theses and Dissertations
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ETD
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Title
Graduate School - New Brunswick Electronic Theses and Dissertations
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rucore19991600001
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NjNbRU
Identifier
(type = doi)
doi:10.7282/T3BC3ZB6
Genre
(authority = ExL-Esploro)
ETD doctoral
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Rights
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The author owns the copyright to this work.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
RightsHolder
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(type = personal)
Name
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Cesario
GivenName
Jeffry
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Permission or license
DateTime
2010-09-24 11:12:30
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Name
Jeffry Cesario
Affiliation
Rutgers University. Graduate School - New Brunswick
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Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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ETD
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application/x-tar
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5529600
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