DescriptionSeparate studies have shown relationships between personality and well-being as well as between gratitude and well-being. More recently, a few studies have examined personality, gratitude, and well-being in concert. One question that has been overlooked, however, is if gratitude might serve as a mediator between personality and psychological well-being. The purpose of this study was to explore if the relationship between personality and psychological well-being might be mediated by gratitude. Two hundred twenty-four college students enrolled in an introductory psychology course in a large, mid-Atlantic public university participated in our study. A shortened version of the Big Five Inventory (BFI-10; Rammstedt & John, 2007) was used to measure five personality characteristics: Neuroticism, Extraversion, Openness, Agreeableness, and Conscientiousness. The Gratitude Questionnaire-6 (GQ-6; McCullough, Emmons, & Tsang, 2002) was used to measure gratitude. Lastly, Ryff’s (1989) 84-item inventory was used to measure overall Psychological Well-Being (PWB) and its six dimensions: Autonomy, Environmental Mastery, Personal Growth, Positive Relations with Others, Purpose in Life, and Self-Acceptance. Multiple regression analyses indicated that gratitude fully mediated the relationship between gender and overall PWB as well as the relationship between the personality characteristic Agreeableness and overall PWB. Multiple regression analyses also indicated that gratitude partially mediated the relationship between the personality characteristic Extraversion and overall PWB. Gratitude was also found to fully or partially mediate the relationships between Extraversion and Agreeableness and some of the dimensions of PWB. Implications for further research are discussed.