RUcore: Rutgers University Community Repository
Transposons
Descriptive
TypeOfResource
Text
TitleInfo
(ID = T-1)
Title
Transposons
SubTitle
natural chromosomal engineers : characterization of AC/DS-induced genomic rearrangements and transposon tagged duplicate genes in maize
Identifier
ETD_2958
Identifier
(type = hdl)
http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000056372
Language
LanguageTerm
(authority = ISO639-2);
(type = code)
eng
Genre
(authority = marcgt)
theses
Subject
(ID = SBJ-1);
(authority = RUETD)
Topic
Plant Biology
Subject
(ID = SBJ-2);
(authority = ETD-LCSH)
Topic
Transposons
Subject
(ID = SBJ-3);
(authority = ETD-LCSH)
Topic
Corn--Biotechnology
Abstract
(type = abstract)
Ac (Activator) is the first autonomous transposon described by McClintock. Its ability to create DSBs (Double Strand Breaks) makes it a powerful mutagen. In the presence of the Ac transposase, its non-autonomous Ds (Dissociation) counterparts are just as mutagenic. Ac and Ds are well known for creating excision footprints that lead mostly to the formation of nonfunctional alleles; less well known is their ability to generate large chromosomal rearrangements. We made use of a mutable allele bz-m39(Ac) to facilitate the selection of such rearrangements. We found Ac can give rise to Ds elements, immobilized Ac elements, adjacent sequence deletions with or without Ac excision, and fractured or one-ended Acs with either deletion or duplication of adjacent sequences. This is the first study to provide a comprehensive view of the types and frequencies of mutations, other than simple excision footprints, that can be produced at a locus by Ac-induced DSBs. Closely linked pairs of transposons, a frequent outcome of Ac/Ds transposition, are even more capable of engineering the genome than single elements. We studied the pair of transposons Ds2(DII) and Ac6067, located 6 kb apart in the bz locus in 9S, and documented the first transpositions of a macrotransposon consisting of both elements plus the intervening chromosomal segment. This transposon pair can break chromosomes at a high frequency and produce other complex rearrangements, including deletions, inversions, and reshuffling of the intertransposon segment. TE (Transposable Element) pairs are adept at restructuring chromosomes and may have been instrumental in reshaping plant genomes. The mutagenesis feature of transposons makes them a great tool to facilitate gene isolation and functional analysis. With the assistance of Ac and Mutator transposons, we characterized two maize duplicate genes, stk1 and stk2. These paralogs express only in pollen and the mature tassel, and both likely function in pollen tube growth since mutations of either one affect pollen transmission, though stk2 shows a milder effect. The double mutant combination is essentially pollen lethal. This study shows that the stk paralogs play an essential, though slightly unequal, role in pollen development.
PhysicalDescription
Form
(authority = gmd)
electronic resource
Extent
xii, 120 p. : ill.
InternetMediaType
application/pdf
InternetMediaType
text/xml
Note
(type = degree)
Ph.D.
Note
(type = bibliography)
Includes bibliographical references
Note
(type = vita)
Includes vita
Note
(type = statement of responsibility)
by Jun Huang
Name
(ID = NAME-1);
(type = personal)
NamePart
(type = family)
Huang
NamePart
(type = given)
Jun
NamePart
(type = date)
1981-
Role
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(authority = RULIB)
author
DisplayForm
Jun Huang
Name
(ID = NAME-2);
(type = personal)
NamePart
(type = family)
Dooner
NamePart
(type = given)
Hugo K
Role
RoleTerm
(authority = RULIB)
chair
Affiliation
Advisory Committee
DisplayForm
Hugo K Dooner
Name
(ID = NAME-3);
(type = personal)
NamePart
(type = family)
Messing
NamePart
(type = given)
Joachim
Role
RoleTerm
(authority = RULIB)
internal member
Affiliation
Advisory Committee
DisplayForm
Joachim Messing
Name
(ID = NAME-4);
(type = personal)
NamePart
(type = family)
Jelenkovic
NamePart
(type = given)
Gojko
Role
RoleTerm
(authority = RULIB)
internal member
Affiliation
Advisory Committee
DisplayForm
Gojko Jelenkovic
Name
(ID = NAME-5);
(type = personal)
NamePart
(type = family)
Mckim
NamePart
(type = given)
Kim
Role
RoleTerm
(authority = RULIB)
outside member
Affiliation
Advisory Committee
DisplayForm
Kim Mckim
Name
(ID = NAME-1);
(type = corporate)
NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
(ID = NAME-2);
(type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm
(authority = RULIB)
school
OriginInfo
DateCreated
(qualifier = exact)
2010
DateOther
(qualifier = exact);
(type = degree)
2010-10
Place
PlaceTerm
(type = code)
xx
RelatedItem
(type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
(type = RULIB)
ETD
RelatedItem
(type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier
(type = local)
rucore19991600001
Location
PhysicalLocation
(authority = marcorg);
(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T3Q2400T
Genre
(authority = ExL-Esploro)
ETD doctoral
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Rights
RightsDeclaration
(AUTHORITY = GS);
(ID = rulibRdec0006)
The author owns the copyright to this work.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
RightsHolder
(ID = PRH-1);
(type = personal)
Name
FamilyName
Huang
GivenName
Jun
Role
Copyright Holder
RightsEvent
(ID = RE-1);
(AUTHORITY = rulib)
Type
Permission or license
DateTime
2010-09-30 10:46:02
AssociatedEntity
(ID = AE-1);
(AUTHORITY = rulib)
Role
Copyright holder
Name
Jun Huang
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
(ID = AO-1);
(AUTHORITY = rulib)
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
(ID = RE-2);
(AUTHORITY = rulib)
Type
Embargo
DateTime
2010-10-31
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after October 31st, 2011.
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Technical
ContentModel
ETD
MimeType
(TYPE = file)
application/pdf
MimeType
(TYPE = container)
application/x-tar
FileSize
(UNIT = bytes)
23603200
Checksum
(METHOD = SHA1)
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