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A novel enhancer of Dbx1 is able to drive neural tissue specific reporter gene expression

Descriptive

TypeOfResource
Text
TitleInfo (ID = T-1)
Title
A novel enhancer of Dbx1 is able to drive neural tissue specific reporter gene expression
Identifier
ETD_2795
Identifier (type = hdl)
http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000056458
Language
LanguageTerm (authority = ISO639-2); (type = code)
eng
Genre (authority = marcgt)
theses
Subject (ID = SBJ-1); (authority = RUETD)
Topic
Biomedical Engineering
Subject (ID = SBJ-2); (authority = ETD-LCSH)
Topic
Central nervous system
Subject (ID = SBJ-3); (authority = ETD-LCSH)
Topic
Genetic regulation
Subject (ID = SBJ-4); (authority = ETD-LCSH)
Topic
Gene expression
Subject (ID = SBJ-5); (authority = ETD-LCSH)
Topic
Mesencephalon
Abstract (type = abstract)
Dbx1 is a homeobox gene that plays an essential role in the patterning of the central nervous system (CNS) during development. Its expression pattern is regionally restricted within the brain and spinal cord and has been linked to defining neuronal-specific domains. Enhancers, a major type of transcriptional regulatory element, are important in determining tissue specific gene expression. Here we have identified a novel enhancer of Dbx1 in order to understand mechanisms of spatial and temporal regulation of the Dbx1 gene. Using a multi-genome sequence alignment tool, a conserved non-coding region, Dbx1CR5 was identified as a putative enhancer of Dbx1. A green fluorescent protein (GFP) reporter assay system was used to verify the ability of Dbx1CR5 to drive neural tissue specific reporter gene expression in the developing chick CNS. GFP was detected between embryonic days 3 and 18 and was restricted to areas of the mesencephalon. Morphology and molecular staining of GFP+ tissues indicate that GFP is expressed in a heterogeneous population of cells. Within Dbx1CR5, multiple regions were identified to individually activate GFP expression in varying spatial patterns. Our findings support the notion that multiple binding elements reside within the 1135bp region of Dbx1CR5. Through this research we hope to identify factors that may one day be used to develop potential and novel therapeutics.
PhysicalDescription
Form (authority = gmd)
electronic resource
Extent
v, 41 p. : ill.
InternetMediaType
application/pdf
InternetMediaType
text/xml
Note (type = degree)
M.S.
Note (type = bibliography)
Includes bibliographical references
Note (type = statement of responsibility)
by Jennifer Kim
Name (ID = NAME-1); (type = personal)
NamePart (type = family)
Kim
NamePart (type = given)
Jennifer
Role
RoleTerm (authority = RULIB)
author
DisplayForm
Jennifer Kim
Name (ID = NAME-2); (type = personal)
NamePart (type = family)
Cai
NamePart (type = given)
Li
Role
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chair
Affiliation
Advisory Committee
DisplayForm
Li Cai
Name (ID = NAME-3); (type = personal)
NamePart (type = family)
Kim
NamePart (type = given)
Sobin
Role
RoleTerm (authority = RULIB)
internal member
Affiliation
Advisory Committee
DisplayForm
Sobin Kim
Name (ID = NAME-4); (type = personal)
NamePart (type = family)
Roth
NamePart (type = given)
Charles
Role
RoleTerm (authority = RULIB)
internal member
Affiliation
Advisory Committee
DisplayForm
Charles Roth
Name (ID = NAME-1); (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (ID = NAME-2); (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB)
school
OriginInfo
DateCreated (qualifier = exact)
2010
DateOther (qualifier = exact); (type = degree)
2010-10
Place
PlaceTerm (type = code)
xx
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T3JM29CP
Genre (authority = ExL-Esploro)
ETD graduate
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Rights

RightsDeclaration (AUTHORITY = GS); (ID = rulibRdec0006)
The author owns the copyright to this work.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
RightsHolder (ID = PRH-1); (type = personal)
Name
FamilyName
Kim
GivenName
Jennifer
Role
Copyright Holder
RightsEvent (ID = RE-1); (AUTHORITY = rulib)
Type
Permission or license
DateTime
2010-07-30 15:28:28
AssociatedEntity (ID = AE-1); (AUTHORITY = rulib)
Role
Copyright holder
Name
Jennifer Kim
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject (ID = AO-1); (AUTHORITY = rulib)
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent (ID = RE-2); (AUTHORITY = rulib)
Type
Embargo
DateTime
2010-10-31
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after October 30th, 2012.
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Technical

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ETD
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application/pdf
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application/x-tar
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6287360
Checksum (METHOD = SHA1)
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