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Arabidopsis mutants revealing novel aspects of leaf morphogenesis
Descriptive
TypeOfResource
Text
TitleInfo
(ID = T-1)
Title
Arabidopsis mutants revealing novel aspects of leaf morphogenesis
Identifier
ETD_2822
Identifier
(type = hdl)
http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000056476
Language
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(authority = ISO639-2);
(type = code)
eng
Genre
(authority = marcgt)
theses
Subject
(ID = SBJ-1);
(authority = RUETD)
Topic
Plant Biology
Subject
(ID = SBJ-2);
(authority = ETD-LCSH)
Topic
Leaves--Morphogenesis
Subject
(ID = SBJ-3);
(authority = ETD-LCSH)
Topic
Arabidopsis
Abstract
(type = abstract)
The establishment of dorsoventrality along the adaxial-abaxial axis is considered an early and crucial step of leaf development and proposed as a requirement for subsequent leaf blade expansion. Because photosynthesis mainly takes place in leaves, several key determinants are required to ensure the proper formation of mature leaves. Several efforts have been dedicated to elucidate the regulatory networks controlling leaf morphogenesis. In this thesis, characterization of novel regulators for both leaf polarity establishment and leaf blade expansion is described. Several Arabidopsis leaf polarity mutants have been identified. However, none of the single, recessive mutants display polarity defects similar to phantastica (phan), the first leaf polarity mutant in Antirrhinum majus. Characterization of filliforme (flr) indicates that flr may be the missing counterpart of phan. flr exhibited the abaxial features in the radialized organs as well as disturbed shoot apical meristem organization. Gene expression analysis revealed the alteration of expression levels of several key regulatory genes. Remarkably, FLR encodes a plastid membrane protein, underlining the roles of plastids in Arabidopsis growth and development. sensitive to red light reduced (srr) 1-2 mutants displayed a narrow-leaf phenotype in addition to defects in light perception and circadian clock regulation. This suggests a connection between leaf blade expansion and the circadian clock. Due to the lack of recognizable functional domains, molecular functions of SRR1 remain unknown. However, genetic interactions between srr1-2 and translational machinery mutants indicate the involvement of SRR1 in the translational regulation of leaf morphogenesis. Previous studies have demonstrated the importance of a translational regulation during leaf morphogenesis in Arabidopsis. Mutations in several ribosomal protein genes similarly result in a pointed-leaf phenotype. Characterization of pointed-first-leaves (pfl) 2-2 mutants, in which the ribosomal protein gene RPS13 is disrupted, revealed perturbation in both adaxial and abaxial mesophyll layers. This mutation in pfl2-2 also significantly enhanced polarity defects in several leaf polarity mutants. These results suggest that RPS13 contributes along with other regulatory genes in establishing or maintaining leaf polarity. Together, genetic approaches present in this dissertation expose unexpected contribution of plastids, the circadian clock and translational machineries on leaf morphogenesis.
PhysicalDescription
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electronic resource
Extent
x, 111 p. : ill.
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application/pdf
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text/xml
Note
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Ph.D.
Note
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Includes bibliographical references
Note
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Includes vita
Note
(type = statement of responsibility)
by Chokchai Kittiwongwattana
Name
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Kittiwongwattana
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Chokchai
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1981-
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author
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Chokchai Kittiwongwattana
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Kerstetter
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Randall
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chair
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Advisory Committee
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Randall Kerstetter
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NamePart
(type = family)
Maliga
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(type = given)
Pal
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internal member
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Advisory Committee
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Pal Maliga
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(type = personal)
NamePart
(type = family)
Dooner
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(type = given)
Hugo
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internal member
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Advisory Committee
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Hugo Dooner
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(type = personal)
NamePart
(type = family)
Padgett
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Richard
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outside member
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Advisory Committee
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Richard Padgett
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NamePart
Rutgers University
Role
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degree grantor
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Graduate School - New Brunswick
Role
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(authority = RULIB)
school
OriginInfo
DateCreated
(qualifier = exact)
2010
DateOther
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(type = degree)
2010-10
Place
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(type = code)
xx
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TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
(type = RULIB)
ETD
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(type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier
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rucore19991600001
Location
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(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T3QC037S
Genre
(authority = ExL-Esploro)
ETD doctoral
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Rights
RightsDeclaration
(AUTHORITY = GS);
(ID = rulibRdec0006)
The author owns the copyright to this work.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
RightsHolder
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(type = personal)
Name
FamilyName
Kittiwongwattana
GivenName
Chokchai
Role
Copyright Holder
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Type
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DateTime
2010-08-25 12:32:50
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Name
Chokchai Kittiwongwattana
Affiliation
Rutgers University. Graduate School - New Brunswick
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License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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Technical
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ETD
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application/pdf
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application/x-tar
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49408000
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