Staff View
Regulation of TGFβ signaling by microRNAs

Descriptive

TypeOfResource
Text
TitleInfo (ID = T-1)
Title
Regulation of TGFβ signaling by microRNAs
Identifier
ETD_2866
Identifier (type = hdl)
http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000056507
Language
LanguageTerm (authority = ISO639-2); (type = code)
eng
Genre (authority = marcgt)
theses
Subject (ID = SBJ-1); (authority = RUETD)
Topic
Cell and Developmental Biology
Subject (ID = SBJ-2); (authority = ETD-LCSH)
Topic
RNA
Subject (ID = SBJ-3); (authority = ETD-LCSH)
Topic
Gene expression
Subject (ID = SBJ-4); (authority = ETD-LCSH)
Topic
Neuroglia
Abstract (type = abstract)
The TGFβ superfamily plays important roles in various processes. With the genetic tools available, Drosophila has been a useful model organism to study the regulators of the TGFβ pathways, which can shed light on potential treatments for many developmental disorders and diseases caused by aberrant TGFβ signaling. microRNAs (miRNAs) are small non-coding RNAs, acting posttranscriptionally to regulate gene expression, that are involved in various aspects of cellular and developmental processes. My thesis work examines the regulation of TGFβ-like pathways by miRNAs. Specifically, with the combination of computational algorithms and tissue culture methods, my early work successfully identified and validated the targets of Drosophila miRNAs. From that, I found that bantam, a miRNA, can down regulate Mad (Mothers against dpp), a signaling component of TGFβ. Furthermore, I used Drosophila as a model and demonstrated that bantam is a negative regulator of the Dpp (decapentaplegic) pathway. My results showed bantam down regulates Mad (Mothers against dpp) expression in vivo by targeting the Mad 3’UTR, resulting in changes in Dpp signaling. The removal of bantam binding sites in the 3’UTR of a Mad transgene results in a significant increase in the viability of haploinsufficient dpp animals compared to a Mad transgene carrying intact bantam binding sites in the 3’UTR. Interestingly, bantam is up-regulated by Dpp in the wing imaginal disc, and thereby functions in a Dpp feedback loop. Furthermore, this feedback loop is important for maintaining anterior-posterior (A/P) compartment boundary stability in the wing disc through regulation of omb (optomotor-blind). Comparative genomics reveal that bantam is evolutionarily conserved, and miRNA target predictions suggest that human bantam homologs selectively target Smad5, one of two homologs of Mad in BMP signaling, but does not target Smad2 which functions in the activin/TGFβ pathway. These data suggest that bantam is a conserved negative regulator of BMP/Dpp signaling. In addition to the work in the wing disc, I extended my studies and examined the role of bantam in Drosophila brain development. My work shows that bantam is critical for maintaining the stem cell pools of the optic lobe, and that bantam has a cell-autonomously effect on glial cell proliferation and distribution, largely through targeting omb.
PhysicalDescription
Form (authority = gmd)
electronic resource
Extent
xii, 220 p. : ill.
InternetMediaType
application/pdf
InternetMediaType
text/xml
Note (type = degree)
Ph.D.
Note (type = bibliography)
Includes bibliographical references
Note (type = vita)
Includes vita
Note (type = statement of responsibility)
by Ying Li
Name (ID = NAME-1); (type = personal)
NamePart (type = family)
Li
NamePart (type = given)
Ying
NamePart (type = date)
1975-
Role
RoleTerm (authority = RULIB)
author
DisplayForm
YING LI
Name (ID = NAME-2); (type = personal)
NamePart (type = family)
Irvine
NamePart (type = given)
Kenneth
Role
RoleTerm (authority = RULIB)
chair
Affiliation
Advisory Committee
DisplayForm
Kenneth Irvine
Name (ID = NAME-3); (type = personal)
NamePart (type = family)
Mckim
NamePart (type = given)
Kim
Role
RoleTerm (authority = RULIB)
internal member
Affiliation
Advisory Committee
DisplayForm
Kim Mckim
Name (ID = NAME-4); (type = personal)
NamePart (type = family)
Driscoll
NamePart (type = given)
Monica
Role
RoleTerm (authority = RULIB)
internal member
Affiliation
Advisory Committee
DisplayForm
Monica Driscoll
Name (ID = NAME-5); (type = personal)
NamePart (type = family)
Padgett
NamePart (type = given)
Richard
Role
RoleTerm (authority = RULIB)
internal member
Affiliation
Advisory Committee
DisplayForm
Richard Padgett
Name (ID = NAME-6); (type = personal)
NamePart (type = family)
Kramer
NamePart (type = given)
Sunita
Role
RoleTerm (authority = RULIB)
outside member
Affiliation
Advisory Committee
DisplayForm
Sunita Kramer
Name (ID = NAME-1); (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (ID = NAME-2); (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB)
school
OriginInfo
DateCreated (qualifier = exact)
2010
DateOther (qualifier = exact); (type = degree)
2010-10
Place
PlaceTerm (type = code)
xx
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T32Z159V
Genre (authority = ExL-Esploro)
ETD doctoral
Back to the top

Rights

RightsDeclaration (AUTHORITY = GS); (ID = rulibRdec0006)
The author owns the copyright to this work.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
RightsHolder (ID = PRH-1); (type = personal)
Name
FamilyName
LI
GivenName
YING
Role
Copyright Holder
RightsEvent (ID = RE-1); (AUTHORITY = rulib)
Type
Permission or license
DateTime
2010-09-15 16:27:00
AssociatedEntity (ID = AE-1); (AUTHORITY = rulib)
Role
Copyright holder
Name
YING LI
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject (ID = AO-1); (AUTHORITY = rulib)
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Back to the top

Technical

ContentModel
ETD
MimeType (TYPE = file)
application/pdf
MimeType (TYPE = container)
application/x-tar
FileSize (UNIT = bytes)
61736960
Checksum (METHOD = SHA1)
f2d76bf4498f6ac8ddb5dcdf6788f7b3126b2423
Back to the top
Version 8.5.5
Rutgers University Libraries - Copyright ©2024